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AAVpro® CRISPR/SaCas9 Helper Free System (AAV2)

Cat. # Product Size License Quantity Details
632619 AAVpro® CRISPR/SaCas9 Helper Free System (AAV2) 1 System USD $876.00

The AAVpro CRISPR/SaCas9 Helper Free System (AAV2) is a complete system for the delivery of genes encoding the components necessary for CRISPR/Cas9-mediated genome editing (i.e., sgRNA and Cas9 nuclease) to mammalian cells. pAAV-Guide-it-1 Vector is supplied prelinearized for simple cloning of a target-specific sgRNA sequence under the control of the human U6 promoter. It also contains the Cas9 gene from Staphylococcus aureus (SaCas9) expressed from an upstream CMV promoter. SaCas9 can edit the genome with efficiencies similar to those of the more commonly used Streptococcus pyogenes (SpCas9), but it is about 1 kb shorter, which allows it to be packaged into a single AAV vector. It is important to note that SaCas9 also recognizes a different PAM sequence; please check the user manual for details. This kit also contains all of the components needed for cloning and viral packaging, including ligation mix, necessary controls, Stellar Competent Cells, and reagents necessary for helper virus-free AAV packaging.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Helper virus-free preparation of AAV particles to deliver Cas9 and sgRNA

Helper virus-free preparation of AAV2 particles to deliver Cas9 and sgRNA to mammalian cells for CRISPR/Cas9 genome editing.

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AAV2 CRISPR/Cas9 system

Helper-free preparation of AAV2 particles for CRISPR/Cas9-mediated gene editing.

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