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AAVpro® CRISPR/Cas9 Vector System

Cat. # Product Size Price License Quantity Details
632609 AAVpro® CRISPR/Cas9 Vector System 1 System USD $502.00

The AAVpro CRISPR/Cas9 Vector System is a complete system for cloning a target single guide RNA (sgRNA) for CRISPR/Cas9-mediated genome editing in mammalian cells. The included AAVpro CRISPR/Cas9 Vector Set consists of two plasmids (pAAV-Guide-it-Up and pAAV-Guide-it-Down) that each contain a truncated, upstream or downstream portion of the Cas9 gene with a 1.6-kb region of homology. Due to the nature of the AAV genome, when target cells are transduced with both viruses (Up and Down), recombination occurs at the region of homology to create a full-length Cas9 gene with an upstream CMV promoter. pAAV-Guide-it-Down is supplied pre-linearized for simple cloning of a target-specific sgRNA sequence under the control of the human U6 promoter. This kit also contains all of the components needed for cloning, including ligation mix, necessary controls, and Stellar Competent Cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Helper virus-free preparation of AAV particles to deliver Cas9 and sgRNA

Helper virus-free preparation of AAV2 particles to deliver Cas9 and sgRNA to mammalian cells for CRISPR/Cas9 genome editing.

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AAV2 CRISPR/Cas9 system

Helper-free preparation of AAV2 particles for CRISPR/Cas9-mediated gene editing.

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