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Lenti-X™ Bicistronic Expression System (Puro)

Cat. # Product Size Price License Quantity Details
632183 Lenti-X™ Bicistronic Expression System (Puro) Each USD $1096.00

The Lenti-X Bicistronic Expression Systems enable you to produce high-titer recombinant lentivirus for the purpose of transferring genes into dividing or non-dividing mammalian cells. The pLVX-IRES-Puro Vector contains an internal ribosome entry site (IRES) followed by a drug resistance marker, so that your gene of interest and puromycin resistance are simultaneously coexpressed from a single mRNA transcript. The Lenti-X packaging system is a proprietary combination of plasmids that collectively expresses all the viral proteins necessary for assembling the vector into infectious virions. Lenti-X Packaging Single Shots, which contain lyophilized Lenti-X packaging plasmids and transfection reagent, are used for efficient introduction of the packaging plasmids and the pLVX-IRES-Puro Vector into Lenti-X 293T Cells (Cat. No. 632180; not included) to produce lentivirus. These optimized reagents allow the transfected Lenti-X 293T cultures to produce high titers of VSV-G pseudotyped lentivirus that is both safe and replication-incompetent, and able to transduce virtually any cell type. The Lenti-X GoStix can be used to determine whether virus production is within a usable range or for selecting the best time to harvest your virus. The system provides enough reagents for 16 packaging reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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