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pEF1alpha-IRES Vector

Cat. #	Product	Size		License	Quantity	Details
631970	pEF1alpha-IRES Vector	10 ug	USD $555.00
pEF1α-IRES is a bicistronic mammalian expression vector that allows simultaneous, constitutive expression of two genes of interest from the same mRNA transcript, even after stable integration of the vector into the host cell genome. Stable, constitutive expression of the bicistronic transcript is driven by the human elongation factor 1 alpha (EF1α) promoter, allowing the investigation of a variety of cellular processes (such as differentiation in primary or stem cells) without the transgene silencing associated with CMV promoters. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Expression of AcGFP1 driven by the EF-1 alpha promoter in stem cell lines is higher than expression driven by the CMV promoter Expression of AcGFP1 driven by the EF-1 alpha promoter in stem cell lines is higher than expression driven by the CMV promoter. The mouse embryonic stem cell lines E14 (Panel A) and D3 (Panel B) were transduced by Lenti-X lentivirus, expressing AcGFP1 either under the control of the CMV promoter or the Elongation factor alpha (EF-1 alpha) promoter. The expression level of AcGFP1 in infected cells five days postinfection was monitored by FACS analysis using the FL1 channel. The expression of AcGFP1 driven by the EF-1 alpha promoter in both stem cell lines was considerably higher compared to the CMV promoter. This is mainly due to a considerably lower rate of silencing of the EF-1 alpha promoter in stem cells compared to the CMV promoter as published (Wang, et al. (2008) Stem Cells Dev 17:279–289). Back Map schematic of the plasmid choices that are available carrying the EF1-alpha promoter Map schematic of the plasmid choices that are available carrying the EF1-alpha promoter. IRES: internal ribosome entry sequence; FP1: fluorescent protein (AcGFP1, DsRed-Monomer, or mCherry); FP2: fluorescent protein (mCherry or ZsGreen1); MCS: multiple cloning site. Back 631970: pEF1alpha-IRES Vector