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Retro-X™ Universal Packaging System

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Product

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Details

631530

Retro-X™ Universal Packaging System

Each

USD $897.00

License Statement

ID Number
8	For Research Use Only, Not for Use in Humans.

This kit provides a complete retroviral vector packaging system for use with any retroviral vector. It is designed to produce virus that can infect dividing cells from a broad range of mammalian and nonmammalian cell types. The GP2-293 Packaging Cell Line contains only the MoMuLV gag and pol genes. The viral envelope portion of the packaging function (env gene) is supplied by transiently co-transfecting one of the Retro-X Universal Packaging Vectors (p10A1, pAmpho, pEco, or pVSV-G) with the retroviral vector. The pQCLIN Retroviral Vector (included in the vector set) can be used as a positive control to constitutively express β-galactosidase in transduced mammalian cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

pRetroQ-mCherry-C1 Vector

Cat. #

Product

Size

License

Quantity

Details

632567

pRetroQ-mCherry-C1 Vector

10 ug

USD $568.00

License Statement

ID Number
8	For Research Use Only, Not for Use in Humans.
44	The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.
69	The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pRetroQ-mCherry-C1 Vector product License Agreement can be found by clicking here.

The pRetroQ-mCherry-C1 Vector is a high-titer, self-inactivating retroviral expression vector designed to eliminate promoter interference from the upstream LTR in the integrated provirus. The vector encodes mCherry; a bright red fluorescent protein tag that was derived from a monomeric mutant of DsRed (mRFP1) by site-directed mutagenesis. Inserting a cDNA in the MCS downstream of the mCherry coding sequence joins your protein of interest to the C-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent retrovirus, we recommend using the Retro-X Universal Packaging System (Cat. No. 631530).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

pRetroQ-mCherry-N1 Vector

Cat. #

Product

Size

License

Quantity

Details

632568

pRetroQ-mCherry-N1 Vector

10 ug

USD $568.00

License Statement

ID Number
8	For Research Use Only, Not for Use in Humans.
44	The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,671,185, 7,9110,714 and 8,664,471.
69	The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,250,298; 7,671,185; 7,910,714; 8,664,471 and 8,679,749.
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pRetroQ-mCherry-N1 Vector product License Agreement can be found by clicking here.

The pRetroQ-mCherry-N1 Vector is a high-titer, self-inactivating retroviral expression vector designed to eliminate promoter interference from the upstream LTR in the integrated provirus. The vector encodes mCherry; a bright red fluorescent protein tag that was derived from a monomeric mutant of DsRed (mRFP1) by site-directed mutagenesis. Inserting a cDNA in the MCS upstream of the mCherry coding sequence joins your protein of interest to the N-terminus of the tag, and allows the fusion protein to be tracked and studied in transduced cells. To package the vector into high-titer, replication-incompetent retrovirus, we recommend using the Retro-X Universal Packaging System (Cat. No. 631530).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Retroviral Tet-On 3G inducible expression systems

Retroviral delivery format for Tet-On 3G inducible expression systems.

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Retro-X™ Universal Packaging System

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pRetroQ-mCherry-C1 Vector

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Retroviral Tet-On 3G inducible expression systems

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Schematic for retroviral packaging using Retro-X Universal Packaging System

Retrovirus packaging options from Clontech

Characteristics of retroviral packaging cell lines: AmphoPack-293, EcoPack 2-293, and RetroPack PT67

High titer retroviral packaging with a choice of four different retroviral packaging envelopes

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Retro-X retroviral vector maps

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Retro-X retroviral vector maps

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