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Ecotropic Receptor Booster

Cat. # Product Size License Quantity Details
631471 Ecotropic Receptor Booster 20 Rxns USD $323.00

The Ecotropic Receptor Booster increases the transduction efficiency of cells that are either resistant to infection, or inefficiently infected by ecotropic pseudotyped lentivirus or retrovirus. The Ecotropic Receptor Booster consists of exosome-like particles coated with the mCAT-1 receptor. When applied to target cells, fusion of the exosome-like particles with the host cell plasma membrane temporarily increases surface mCAT-1 receptor, thus allowing for efficient viral transduction.The Ecotropic Receptor Booster renders cells that cannot usually be transduced with ecotropic psuedotyped virus (e.g. human celllines) susceptible to viral infection.

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Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Required Products

Cat. # Product Size Price License Quantity Details
631507 EcoPack™ 2-293 Cell Line 1 mL Inquire for Quotation *

A cell line derived from human embryonic kidney (HEK 293). The cells are designed for rapid production of high-titer Moloney-based retroviral stocks by transient transfection, and are intended for use with retroviral vectors and related technology for functional expression analysis. Virus produced by these cells can infect mouse and rat cells.

631530 Retro-X™ Universal Packaging System Each USD $897.00

This kit provides a complete retroviral vector packaging system for use with any retroviral vector. It is designed to produce virus that can infect dividing cells from a broad range of mammalian and nonmammalian cell types. The GP2-293 Packaging Cell Line contains only the MoMuLV gag and pol genes. The viral envelope portion of the packaging function (env gene) is supplied by transiently co-transfecting one of the Retro-X Universal Packaging Vectors (p10A1, pAmpho, pEco, or pVSV-G) with the retroviral vector. The pQCLIN Retroviral Vector (included in the vector set) can be used as a positive control to constitutively express β-galactosidase in transduced mammalian cells.

631251 Lenti-X™ HTX Ecotropic Packaging System 20 Rxns USD $1076.00

The Lenti-X HTX Ecotropic Packaging System allows you to produce very high virus titers from lentiviral vectors for the purposes of transferring exogenous genes into cells and tissues. The Lenti-X HTX Ecotropic Packaging Mix 2 is a proprietary combination of plasmids that expresses the viral proteins necessary for high-efficiency packaging of lentiviral vector transcripts into infectious, ecotropically-pseudotyped, lentiviral particles. The MLV ecotropic envelope glycoprotein enables mouse and rat cells to be transduced with high efficiency. The biodegradable nanoparticle-based Xfect transfection reagent transfers the packaging mix DNA and your lentiviral vector into Lenti-X 293T cells (Cat. No. 632180) with very high efficiency. It is the specific combination of these rigorously optimized components that allows your 293T cell cultures to produce extremely high titers of safe, replication-incompetent lentivirus from any of our Lenti-X System vectors, or from any other lentiviral vector. The Lenti-X GoStix can be used to determine whether virus production is within a usable range or for selecting the best time to harvest your virus.

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Ecotropic Receptor Booster

Increase retrovirus transduction efficiency by elevating the density of ecotropic receptor proteins on target cells.

Showing 1-2 of 2 results