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Simple, rapid protein transfection with Xfect Protein Transfection Reagent

Simple, rapid protein transfection with Xfect Protein Transfection Reagent. Xfect’s reagent cell-penetrating activity enables proteins to be transported across membranes of mammalian cells.

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How fast is direct protein transfection?

How fast is direct protein transfection?. We transfected human recombinant caspase-3 using Xfect Protein Transfection Reagent and detected completion of apoptosis after just 2 hr, demonstrating that protein transfection using Xfect reagent is both fast and delivers active protein (Panel B). Other methods for inducing apoptosis require 12 hr for completion of Annexin V staining (Martin, S. J. et al. (1995) J. Exp. Med. 182(5):1545–1556.).

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Xfect Protein Transfection Reagent delivers up to 8X more active beta-galactosidase into HeLa cells than the leading competitor, Product C.

Xfect Protein Transfection Reagent delivers up to 8X more active beta-galactosidase into HeLa cells than the leading competitor, Product C. Panel A. Xfect Protein Transfection Reagent or Product C was used to transfect Jurkat cells with various amount of beta-galactosidase (beta-gal) according to the manufacturers’ recommended protocol. One hour post-transfection, the cells were assayed for beta-gal activity using ourLuminescent beta-galactosidase Detection Kit II (Cat. No. 631712). Xfect Protein Transfection Reagent displayed a far higher signal for beta-gal than did Product C. Panel B. HeLa cells transfected with 4 µg of beta-gal demonstrated higher efficiency and a higher amount of beta-gal protein per cell when transfection was performed with Xfect Protein Transfection Reagent (middle panel) than with Product C (right-hand panel).

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Xfect Protein Transfection Reagent delivers more recombinant fluorescent proteins than Product C into a range of cell types

Xfect Protein Transfection Reagent delivers more recombinant fluorescent proteins than Product C into a range of cell types. Xfect Protein Transfection Reagent and Product C were used to transfect a number of cell lines with purified recombinant fluorescent protein (either rAcGFP1 or rDsRed-Express). In all of the cell lines tested, a far greater MFI (mean fluorescence intensity) was detected via flow cytometry after transfection with Xfect Protein Transfection Reagent than after transfection with Product C.

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Xfect Protein Transfection Reagent provides much higher cell viability than Product P when transfecting HeLa cells

Xfect Protein Transfection Reagent provides much higher cell viability than Product P when transfecting HeLa cells. HeLa cells were seeded in a 96-well plate (3 x 10⁴ cells per well) one day prior to transfection. The cells were transfected with 0.25 µg of beta-galactosidase using Product P or Xfect Protein Transfection Reagent for 4 hours. A WST-1 proliferation assay was performed to assess cytotoxicity in the transfected samples relative to untreated HeLa cells.

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Protein transfection efficiencies across different cell lines: Xfect Protein Transfection Reagent vs. the leading competitor, Product C

Protein transfection efficiencies across different cell lines: Xfect Protein Transfection Reagent vs. the leading competitor, Product C. Panel A. Xfect Protein Transfection Reagent yields higher transfection efficiencies than Product C across a broad range of mammalian cells, including a number of rodent and human cell lines, hard-to-transfect human suspension cells, and mouse embryonic stem cells. The cells were transfected with 4 µg of recombinant fluorescent protein (either rAcGFP1 or rDsRed-Express) using Xfect Protein Transfection Reagent or Product C according to the manufacturers’ recommended protocol. Transfection efficiency was assessed by flow cytometry one hour post-transfection. Panel B. HeLa transfected with 5 µg of rAcGFP1 using Product C or Xfect Protein Transfection Reagent.

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631324: Xfect Protein Transfection Reagent