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Transduction with Lenti-X Accelerator versus Polybrene

Transduction with Lenti-X Accelerator versus Polybrene. Placing virus-bound magnetic beads in a magnetic field greatly increases the localized virus concentration at the surface of the cell monolayer. This reduces the transduction time to just 5 min after a 20 min preincubation to bind the beads to the virus—compared to transduction overnight with Polybrene. Accelerated transduction also limits exposure of your sensitive target cells to viral supernatant.

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Lenti-X Accelerator (25 min) is faster than Polybrene (16 h) for lentiviral transduction

Lenti-X Accelerator (25 min) is faster than Polybrene (16 h) for lentiviral transduction. A ZsGreen1-expressing Lenti-X vector was used to transduce HT1080 cells. Lenti-X Accelerator beads (8 µl) were preincubated for 20 min at room temperature with 200 µl (approx. 1x10⁶ total IFU) of viral supernatant and applied to HT1080 cells on a Magnetic Separator for 5 min. HT1080 cells were also transduced with the same amount of virus in the presence of 6 µg/ml Polybrene, for 5 min or overnight. After the cultures were grown for an additional 72 hr at 37°C, the number of transduced cells was determined by flow cytometry

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Magnetic separator for 96-well, 6-well, 24-well, and 12-well cell culture dishes

Magnetic separator for 96-well, 6-well, 24-well, and 12-well cell culture dishes.

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631254: Lenti-X Accelerator Starter Kit

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Transduction with Lenti-X Accelerator versus Polybrene

Transduction with Lenti-X Accelerator versus Polybrene. Placing virus-bound magnetic beads in a magnetic field greatly increases the localized virus concentration at the surface of the cell monolayer. This reduces the transduction time to just 5 min after a 20 min preincubation to bind the beads to the virus—compared to transduction overnight with Polybrene. Accelerated transduction also limits exposure of your sensitive target cells to viral supernatant.

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Lenti-X Accelerator (25 min) is faster than Polybrene (16 h) for lentiviral transduction

Lenti-X Accelerator (25 min) is faster than Polybrene (16 h) for lentiviral transduction. A ZsGreen1-expressing Lenti-X vector was used to transduce HT1080 cells. Lenti-X Accelerator beads (8 µl) were preincubated for 20 min at room temperature with 200 µl (approx. 1x10⁶ total IFU) of viral supernatant and applied to HT1080 cells on a Magnetic Separator for 5 min. HT1080 cells were also transduced with the same amount of virus in the presence of 6 µg/ml Polybrene, for 5 min or overnight. After the cultures were grown for an additional 72 hr at 37°C, the number of transduced cells was determined by flow cytometry

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Magnetic separator for 96-well, 6-well, 24-well, and 12-well cell culture dishes

Magnetic separator for 96-well, 6-well, 24-well, and 12-well cell culture dishes.

Back

631256: Lenti-X Accelerator

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Transduction with Lenti-X Accelerator versus Polybrene

Transduction with Lenti-X Accelerator versus Polybrene. Placing virus-bound magnetic beads in a magnetic field greatly increases the localized virus concentration at the surface of the cell monolayer. This reduces the transduction time to just 5 min after a 20 min preincubation to bind the beads to the virus—compared to transduction overnight with Polybrene. Accelerated transduction also limits exposure of your sensitive target cells to viral supernatant.

Back

Lenti-X Accelerator (25 min) is faster than Polybrene (16 h) for lentiviral transduction

Lenti-X Accelerator (25 min) is faster than Polybrene (16 h) for lentiviral transduction. A ZsGreen1-expressing Lenti-X vector was used to transduce HT1080 cells. Lenti-X Accelerator beads (8 µl) were preincubated for 20 min at room temperature with 200 µl (approx. 1x10⁶ total IFU) of viral supernatant and applied to HT1080 cells on a Magnetic Separator for 5 min. HT1080 cells were also transduced with the same amount of virus in the presence of 6 µg/ml Polybrene, for 5 min or overnight. After the cultures were grown for an additional 72 hr at 37°C, the number of transduced cells was determined by flow cytometry

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Magnetic separator for 96-well, 6-well, 24-well, and 12-well cell culture dishes

Magnetic separator for 96-well, 6-well, 24-well, and 12-well cell culture dishes.

Back

631257: Lenti-X Accelerator