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Lenti-X™ Provirus Quantitation Kit

Cat. #	Product	Size		License	Quantity	Details
631239	Lenti-X™ Provirus Quantitation Kit	200 Rxns	USD $552.00
The Lenti-X Provirus Quantitation Kit allows you to rapidly determine the provirus content of cells that have been transduced with lentivirus. The kit provides components to perform real-time quantitative PCR of integrated proviruses in genomic DNA, and can be used to determine the copy number of proviruses present in a mixed or clonal population of transduced cells. The kit includes a genomic DNA purification kit, and all the reagents required to conduct 200 TB Green-based qPCR reactions using either a calibrated control template or purified cellular genomic DNA. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Measuring lentiviral copy number in target cells Measuring lentiviral copy number in target cells. Provirus content is determined using a rapid qPCR method. Back Lentiviral provirus copy number affects transgene expression levels in transduced cells Lentiviral provirus copy number affects transgene expression levels in transduced cells. HT1080 cells were infected at either high or low MOI using an AcGFP1 lentiviral expression vector. 72 hr after infection, the cells were subjected to puromycin drug selection. One week later, stably transduced clones were selected for expansion and analysis. AcGFP1 expression levels were determined using flow cytometry, and provirus content in genomic DNA was determined using the Lenti-X Provirus Quantitation Kit. The figure represents data pooled from two groups having either low copy number (>3; n = 8) or high copy number (>9; n = 7). Higher provirus contents were associated with higher expression levels. Back Equivalent virus amounts in different cell types result in different provirus (integrated lentivirus) copy numbers Equivalent virus amounts in different cell types results in different provirus (integrated lentivirus) copy numbers. Equivalent amounts of a single lentiviral stock (100 µl) were used to infect either HT1080, HeLa, and HEK 293 cells. 72 hr after infection, the cells were subjected to drug selection with puromycin. One week later, the provirus copy numbers of the stably transduced cell populations were determined using the Lenti-X Provirus Quantitation Kit. Back 631239: Lenti-X Provirus Quantitation Kit