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Lenti-X™ Maxi Purification Kit

Cat. #	Product	Size		License	Quantity	Details
631233	Lenti-X™ Maxi Purification Kit	2 Preps	USD $299.00
The Lenti-X Maxi Purification Kit purifies lentivirus from crude supernatants, and produces outstanding yields of highly purified virus. The gravity column-based protocol is fast, simple, and effective. The Lenti-X column gently retains charged virus particles from the supernatant, while unbound material exits the column during the wash steps. The purified virus that is eluted from the column is recovered fully intact and fully functional. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Resources Back Lentivirus purification Lentivirus purification.The Lenti-X gravity column-based method (bind, wash, elute) is extremely simple, effective, and preserves virus much better than syringe filter-based methods. Back Clontech's lentiviral purifcation kit generates high yield and high purity Clontech's lentiviral purification kit generates high yield and high purity. Lentivirus was generated using our high-titer lentiviral packaging system and purified. Panel A. To determine yield the lentiviral titer was measured before and after purification using either titration by qRT-PCR (RNA copies) or flow cytometry/fluorescence (IFU). The mean values from seven experiments are shown. Panel B. Equivalent amounts of purified lentivirus (1 x 10⁵ IFU) prepared using either Lenti-X or a filter-based system (Vendor M & Vendor S) were subjected to SDS-PAGE and silver-stained. The Lenti-X sample clearly contained far fewer contaminating proteins at equivalent IFU than either sample prepared from the filter-based systems. Back Collection Rack L Collection Rack L. Once assembled, the rack holds three columns (e.g., lentiviral purification columns) and three collection tubes (50 ml conical tubes) safely and securely. Back 631233: Lenti-X Maxi Purification Kit