LOCUS pZsGreen1-DR 4251 bp DNA circular 9-DEC-2009 COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com COMMENT Cat. No. 632428 FEATURES Location/Qualifiers CDS 2147..2941 /codon_start=1 /transl_table=11 /product="neomycin phosphotransferase" /protein_id="AAB02575.1" /db_xref="GI:1377913" /label=Kan(R)/Neo(R) /note="Kanamycin/neomycin resistance gene Neomycin phosphotransferase coding sequences: start codon (ATG): 2629-2631; stop codon: 3421-3423; G to A mutation to remove Pst I site: 2811; C to A mutation to remove BssH II site: 3157" polyA_signal 1070..1104 /label=SV40\early\polyA\signals /note="SV40 early mRNA polyadenylation signal Polyadenylation signals: 1552-1557 & 1581-1586; mRNA 3' ends: 1590 & 1602" polyA_signal 3177..3195 /label=HSV\TK\polyA\signals /note="Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal Polyadenylation signals: 3659-3664 & 3672-3677" promoter 1794..2025 /label=SV40\early\Promoter/Enhancer /note="SV40 early promoter Enhancer (72-bp tandem repeats): 2278-2349 & 2350-2421; 21-bp repeats: 2425-2445, 2446-2466, & 2468-2488; Early promoter element: 2501-2507; Major transcription start points: 2497, 2535, 2541, & 2546" promoter 1684..1712 /label=Bacterial\Promoter\for\Kan(R) /note="Bacterial promoter for expression of Kan R gene; -35 region: 2166-2171; -10 region: 2189-2194; Transcription start point: 2201" rep_origin complement(1167..1622) /label=f1\origin /note="f1 single-strand DNA origin Packages the noncoding strand of EGFP)" rep_origin 1963..2098 /label=SV40\origin /note="SV40 origin of replication" rep_origin 3526..4169 /label=pUC\origin CDS 90..914 /label=ZsGreen1-DR misc_feature 12..83 /label=MCS BASE COUNT 983 a 1175 c 1148 g 945 t ORIGIN 1 tagttattac tagcgctacc ggactcagat ctcgagctca agcttcgaat tctgcagtcg 61 acggtaccgc gggcccggga tccgccacca tggcccagtc caagcacggc ctgaccaagg 121 agatgaccat gaagtaccgc atggagggct gcgtggacgg ccacaagttc gtgatcaccg 181 gcgagggcat cggctacccc ttcaagggca agcaggccat caacctgtgc gtggtggagg 241 gcggcccctt gcccttcgcc gaggacatct tgtccgccgc cttcatgtac ggcaaccgcg 301 tgttcaccga gtacccccag gacatcgtcg actacttcaa gaactcctgc cccgccggct 361 acacctggga ccgctccttc ctgttcgagg acggcgccgt gtgcatctgc aacgccgaca 421 tcaccgtgag cgtggaggag aactgcatgt accacgagtc caagttctac ggcgtgaact 481 tccccgccga cggccccgtg atgaagaaga tgaccgacaa ctgggagccc tcctgcgaga 541 agatcatccc cgtgcccaag cagggcatct tgaagggcga cgtgagcatg tacctgctgc 601 tgaaggacgg tggccgcttg cgctgccagt tcgacaccgt gtacaaggcc aagtccgtgc 661 cccgcaagat gcccgactgg cacttcatcc agcacaagct gacccgcgag gaccgcagcg 721 acgccaagaa ccagaagtgg cacctgaccg agcacgccat cgcctccggc tccgccttgc 781 ccaagcttcc gcggagccat ggcttcccgc cggcggtggc ggcgcaggat gatggcacgc 841 tgcccatgtc ttgtgcccag gagagcggga tggaccgtca ccctgcagcc tgtgcttctg 901 ctaggatcaa tgtgtaggcg gccgcgactc tagatcataa tcagccatac cacatttgta 961 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg 1021 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat 1081 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 1141 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc 1201 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc 1261 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag 1321 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga 1381 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc 1441 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa 1501 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt 1561 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc 1621 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 1681 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 1741 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt 1801 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 1861 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 1921 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 1981 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 2041 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 2101 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 2161 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 2221 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 2281 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc 2341 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 2401 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 2461 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 2521 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 2581 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 2641 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt 2701 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 2761 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 2821 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 2881 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 2941 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat 3001 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc 3061 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg 3121 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata 3181 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc 3241 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt 3301 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac 3361 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta 3421 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 3481 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 3541 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 3601 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 3661 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc 3721 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 3781 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 3841 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 3901 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 3961 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 4021 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 4081 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 4141 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 4201 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca t //