LOCUS pDsRed-Monomer-N1 4691 bp DNA circular 12-NOV-2009 COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com COMMENT Cat. No. 632465 FEATURES Location/Qualifiers CDS 2587..3381 /codon_start=1 /transl_table=11 /product="neomycin phosphotransferase" /protein_id="AAB02575.1" /db_xref="GI:1377913" /label=Kan(R)/Neo(R) /note="Kanamycin/neomycin resistance gene Neomycin phosphotransferase coding sequences: start codon (ATG): 2629-2631; stop codon: 3421-3423; G to A mutation to remove Pst I site: 2811; C to A mutation to remove BssH II site: 3157" promoter 1..589 /label=CMV\IE\Promoter /note="Human cytomegalovirus (CMV) immediate early promoter: Enhancer region: 59-465; TATA box: 554-560;Transcription start point: 583; C->G mutation to remove Sac I site: 569" rep_origin complement(1607..2062) /label=f1\origin /note="f1 single-strand DNA origin Packages the noncoding strand of EGFP)" rep_origin 2403..2538 /label=SV40\origin /note="SV40 origin of replication" rep_origin 3966..4609 /label=pUC\origin /note="pUC plasmid replication origin" CDS 679..1353 /label=DsRed-Monomer polyA_signal 1510..1515 /label=SV40\early\polyA\signal polyA_signal 1539..1544 /label=SV40\early\polyA\signal polyA_signal 3617..3622 /label=HSV\TK\polyA\signal polyA_signal 3630..3635 /label=HSV\TK\polyA\signal misc_feature 672..682 /label=Kozak\sequence misc_feature 2236..2465 /label=SV40\Promoter/Enhancer misc_feature 2124..2152 /label=Kan(R)\Promoter BASE COUNT 1118 a 1273 c 1245 g 1055 t ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 661 gatccaccgg tcgccaccat ggacaacacc gaggacgtca tcaaggagtt catgcagttc 721 aaggtgcgca tggagggctc cgtgaacggc cactacttcg agatcgaggg cgagggcgag 781 ggcaagccct acgagggcac ccagaccgcc aagctgcagg tgaccaaggg cggccccctg 841 cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggc ctacgtgaag 901 caccccgccg acatccccga ctacatgaag ctgtccttcc ccgagggctt cacctgggag 961 cgctccatga acttcgagga cggcggcgtg gtggaggtgc agcaggactc ctccctgcag 1021 gacggcacct tcatctacaa ggtgaagttc aagggcgtga acttccccgc cgacggcccc 1081 gtaatgcaga agaagactgc cggctgggag ccctccaccg agaagctgta cccccaggac 1141 ggcgtgctga agggcgagat ctcccacgcc ctgaagctga aggacggcgg ccactacacc 1201 tgcgacttca agaccgtgta caaggccaag aagcccgtgc agctgcccgg caaccactac 1261 gtggactcca agctggacat caccaaccac aacgaggact acaccgtggt ggagcagtac 1321 gagcacgccg aggcccgcca ctccggctcc cagtagagcg gccgcgactc tagatcataa 1381 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc 1441 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata 1501 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 1561 attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt 1621 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 1681 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 1741 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 1801 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 1861 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 1921 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 1981 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 2041 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 2101 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 2161 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga 2221 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 2281 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 2341 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 2401 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 2461 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 2521 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg 2581 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg 2641 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg 2701 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat 2761 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca 2821 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg 2881 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat 2941 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa 3001 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg 3061 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg 3121 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg 3181 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat 3241 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac 3301 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc 3361 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc 3421 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg 3481 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt 3541 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa 3601 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc 3661 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt 3721 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag 3781 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat 3841 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 3901 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 3961 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 4021 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 4081 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 4141 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 4201 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 4261 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 4321 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 4381 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 4441 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 4501 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 4561 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 4621 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 4681 ccgccatgca t //