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Technical notes Single-cell cloning of hiPS cells
Technical notes Editing hiPS cells using gesicle technology
Technical notes Editing hiPS cells using electroporation
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Technical notes Single-cell cloning of hiPS cells
Technical notes Editing hiPS cells using gesicle technology
Technical notes Editing hiPS cells using electroporation

Efficient generation of edited hiPS clonal populations

Single-cell cloning of edited stem cells

Establishing clonal populations from edited hiPS cells grown and passaged as colonies is inefficient and time-consuming; often, it results in cell death or premature differentiation. The Cellartis iPSC Single-Cell Cloning DEF-CS Culture Media Kit is a complete system for efficient expansion and scale-up manufacturing of hiPS cells in a feeder-free and defined environment. The kit contains all the necessary reagents to go from seeding single hiPS cells into 96-well plates to expansion of clones into 48-well plates. Following clonal expansion, hiPS cells can continue to be cultured using the Cellartis DEF-CS 500 Culture System.

Establishing clonal populations from edited hiPS cells grown and passaged as colonies is inefficient and time-consuming; often, it results in cell death or premature differentiation. The Cellartis iPSC Single-Cell Cloning DEF-CS Culture Media Kit is a complete system for efficient expansion and scale-up manufacturing of hiPS cells in a feeder-free and defined environment. The kit contains all the necessary reagents to go from seeding single hiPS cells into 96-well plates to expansion of clones into 48-well plates. Following clonal expansion, hiPS cells can continue to be cultured using the Cellartis DEF-CS 500 Culture System.

To generate your own edited clonal hiPS cell lines from start to finish, an electroporation-based or gesicle-based Cas9-sgRNA RNP complex delivery system can be combined with the Cellartis iPSC Single-Cell Cloning DEF-CS Culture Media Kit. Please refer to the following technotes to learn more:

  • Editing iPS cells using electroporation »
  • Editing iPS cells using gesicle technology »
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Cat. # Product Size License Quantity Details
Y30021 Cellartis® iPSC Single-Cell Cloning DEF-CS™ Culture Media Kit 1 Kit USD $744.00

License Statement

ID Number  
C001 This product is manufactured and sold by Takara Bio Europe AB based on a commercial license to certain intellectual property rights held by Wisconsin Alumni Research Foundation (“WARF”). This product is covered by one or more claims of U.S. Patent No. 7,514,260 and its foreign counterparts. The purchase of this product conveys to the buyer the non-transferable right to use the product for its intended use, strictly limited to purchaser’s own internal research. No other express or implied license is granted to the purchaser. Purchaser cannot have any right to use this product or its components in humans for any purposes including but not limited to diagnostics and/or therapeutics, or otherwise clinical trials. Purchase does not include any right to resell or transfer this product to a third party regardless of whether or not compensation is received. Purchasers wishing to use this product for purposes other than internal research use should contact us.

The Cellartis iPSC Single-Cell Cloning DEF-CS Culture Media Kit is a defined culture system for the efficient generation
of human pluripotent stem cell clones from seeded single cells. The kit includes basal medium, coating substrate, and
additives.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Y30021: Cellartis iPSC Single-Cell Cloning DEF-CS Culture Media Kit

Y30021: Cellartis iPSC Single-Cell Cloning DEF-CS Culture Media Kit

Overview

  • Superior single-cell survival—edited hiPS cells exhibit high (typically ~50%) survival when seeded as single cells in a 96-well plate
  • Maintenance of pluripotency during single-cell cloning—hiPS cells maintain high levels (>90%) of pluripotency markers Oct-4, TRA-1-60, and SSEA-4
  • Stable karyotype from start to finish—hiPS cells maintain a normal and stable karyotype throughout single-cell cloning and expansion
  • CRISPR/Cas9 gene-editing solutions available—use our electroporation-based or gesicle-based CRISPR/Cas9 techniques prior to single-cell cloning for a start-to-finish solution for generating clonal hiPS cell lines

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Video protocol: editing human iPSCs with CRISPR/Cas9 and single-cell cloning

In this video protocol, we provide step-by-step instructions for generating edited human iPS cell lines using electroporation-based delivery of CRISPR/Cas9 RNP complexes and single-cell cloning. This high-efficiency workflow greatly improves single-cell survival post-seeding, thanks to the Cellartis DEF-CS culture system.

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Video protocol: editing human iPSCs with CRISPR/Cas9 and single-cell cloning

In this video protocol, we provide step-by-step instructions for generating edited human iPS cell lines using electroporation-based delivery of CRISPR/Cas9 RNP complexes and single-cell cloning. This high-efficiency workflow greatly improves single-cell survival post-seeding, thanks to the Cellartis DEF-CS culture system.

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