Establishing clonal populations from edited hiPS cells grown and passaged as colonies is inefficient and time-consuming; often, it results in cell death or premature differentiation. The Cellartis iPSC Single-Cell Cloning DEF-CS Culture Media Kit is a complete system for efficient expansion and scale-up manufacturing of hiPS cells in a feeder-free and defined environment. The kit contains all the necessary reagents to go from seeding single hiPS cells into 96-well plates to expansion of clones into 48-well plates. Following clonal expansion, hiPS cells can continue to be cultured using the Cellartis DEF-CS 500 Culture System.
Establishing clonal populations from edited hiPS cells grown and passaged as colonies is inefficient and time-consuming; often, it results in cell death or premature differentiation. The Cellartis iPSC Single-Cell Cloning DEF-CS Culture Media Kit is a complete system for efficient expansion and scale-up manufacturing of hiPS cells in a feeder-free and defined environment. The kit contains all the necessary reagents to go from seeding single hiPS cells into 96-well plates to expansion of clones into 48-well plates. Following clonal expansion, hiPS cells can continue to be cultured using the Cellartis DEF-CS 500 Culture System.
To generate your own edited clonal hiPS cell lines from start to finish, an electroporation-based or gesicle-based Cas9-sgRNA RNP complex delivery system can be combined with the Cellartis iPSC Single-Cell Cloning DEF-CS Culture Media Kit. Please refer to the following technotes to learn more:
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
In this video protocol, we provide step-by-step instructions for generating edited human iPS cell lines using electroporation-based delivery of CRISPR/Cas9 RNP complexes and single-cell cloning. This high-efficiency workflow greatly improves single-cell survival post-seeding, thanks to the Cellartis DEF-CS culture system.
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Video protocol: editing human iPSCs with CRISPR/Cas9 and single-cell cloning
In this video protocol, we provide step-by-step instructions for generating edited human iPS cell lines using electroporation-based delivery of CRISPR/Cas9 RNP complexes and single-cell cloning. This high-efficiency workflow greatly improves single-cell survival post-seeding, thanks to the Cellartis DEF-CS culture system.
