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Cellartis MSC EV Wonder

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Cellartis MSC EV Wonder is a xeno-free medium optimized for robust and consistent production of mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs). It enables a seamless transition from expansion to production with no adaptation needed. This medium delivers high EV yields, low background particles, strong CD9/CD81/CD63 expression, and defined particle size for high-performance EVs. 

Cellartis MSC EV Wonder is a xeno-free medium optimized for robust and consistent production of mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs). It enables a seamless transition from expansion to production with no adaptation needed. This medium delivers high EV yields, low background particles, strong CD9/CD81/CD63 expression, and defined particle size for high-performance EVs.

The MSC EV Wonder system consists of two components: a basal xeno-free medium and a chemically defined, EV-specific supplement. Together, they form a complete, optimized solution for high-yield EV production in a streamlined format.

This medium is built to consistently deliver high EV output with excellent purity, helping you achieve more reliable and reproducible results. Its clean formulation supports easy downstream processing and reduces background interference. MSC EV Wonder is an ideal choice for researchers looking to efficiently produce high-quality MSC-derived EVs for advanced research and translational development.

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Cat. # Product Size Price License Quantity Details
Y51000 Cellartis® MSC EV Wonder™ Each USD $680.00

Cellartis® MSC EV Wonder™ is a xeno-free medium designed for the efficient production of extracellular vesicles (EVs) from human mesenchymal stem cells (MSCs). It consists of a xeno-free basal medium and EV supplement, which are combined at a 1:20 ratio to create the complete medium. This product supports robust EV yield, high purity, and reproducibility across MSC sources, including bone marrow (BM-MSCs), adipose tissue (AD-MSCs), and umbilical cord (UC-MSCs). Its phenol red–free formulation makes it ideal for downstream analysis and EV-based research. This medium is a great choice for scientists seeking consistency, scalability, and functional EV output in advanced research applications.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Improved EV production over competitor medium as measured by NanoFCM

Improved EV production over competitor medium as measured by NanoFCM

Improved EV production over competitor medium as measured by NanoFCM. MSCs were cultured in Cellartis MSC Xeno-Free Culture Medium, followed by EV production with Cellartis MSC EV Wonder for five days. A ninefold increase in particle concentration was observed with MSC EV Wonder as compared to DMEM.

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Improved EV production detected using canonical EV surface markers CD9 and CD81

Improved EV production detected using canonical EV surface markers CD9 and CD81

Improved EV production detected using canonical EV surface markers CD9 and CD81. Percentages indicate CD9⁺/CD81⁺ double-positive particles. When MSCs were cultured in Cellartis MSC XF medium and EVs produced using MSC EV Wonder, approximately 97% of particles were CD9⁺/CD81⁺ double positive (as measured by NanoFCM), indicating a high degree of EV purity. These results confirm the ability of MSC EV Wonder to support the generation of well-defined, high-quality vesicles suitable for downstream applications.

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Consistent production of well-defined EVs

Consistent production of well-defined EVs

Consistent production of well-defined EVs. The mean particle size is approximately 65–70 nm, matching the expected size and round morphology of EVs. These results highlight the reliability of the protocol for generating uniform and high-quality MSC-derived EVs.

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Improved EV production over competitor medium detected using EV surface marker CD63

Improved EV production over competitor medium detected using EV surface marker CD63

Improved EV production over competitor medium detected using EV surface marker CD63. EVs produced using Cellartis MSC EV Wonder show a significantly higher signal for CD63, a key exosomal marker associated with vesicle identity and functional potential. MSC EV Wonder demonstrated increased CD63 expression (as measured by ELISA) compared to a leading commercial medium, confirming superior exosome quality and marker abundance.

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Y51000: Cellartis MSC EV Wonder

Y51000: Cellartis MSC EV Wonder

Overview

High-performance medium for MSC-derived EV production that is engineered for consistency, safety, and yield—Cellartis MSC EV Wonder empowers scalable, clinically relevant EV manufacturing. Whether for advanced research or translational workflows, Cellartis MSC EV Wonder delivers results you can trust. 

  • Xeno-free formulation—ensures consistent results
  • Phenol red-free—enables easy downstream EV analysis
  • Seamless expansion to production—eliminates the need for additional optimization
  • High EV yield—maximizes EV output
  • Multi-source MSC support—demonstrates high performance with MSCs derived from bone marrow, adipose tissues, and umbilical cords
  • Low particle background—delivers cleaner isolations with reduced interference

Easy-to-follow workflow for efficient MSC-derived EV production. Minimize variability and streamline your EV workflow with a simple two-phase process using Cellartis MSC Xeno-Free Culture Medium (Cat. # Y50200) for MSC expansion and Cellartis MSC EV Wonder (Cat. # Y51000) for EV production.  

More Information

Applications

MSC-derived EVs are widely studied for their regenerative, immunomodulatory, and therapeutic delivery properties. Their natural bioactivity and safety profile make them valuable in both academic research and early-stage clinical development, particularly in the following areas:

  • Regenerative medicine studies, including tissue repair and wound healing
  • Cell-free therapy development using MSC-derived EVs
  • EV-based drug delivery research, leveraging MSC-derived EVs as natural carriers
  • Immunomodulatory research for autoimmune and inflammatory diseases
  • Neurodegenerative disease models, including stroke, ALS, and Parkinson’s
  • Cardiovascular disease models exploring the protective effects of MSC-derived EVs
  • Oncology research for tumor modulation and drug resistance studies
  • Liver disease and fibrosis research using MSC-derived EV signaling
  • Acute kidney injury and other organ protection studies
  • Graft-versus-host disease (GVHD) models in transplantation research

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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