NDiff N2 cell-culture supplements

NDiff N2 medium is a proprietary, completely defined, serum-free cell-culture supplement for the derivation, maintenance, expansion, and/or differentiation of human and mouse stem cells and tissue-derived neural stem (NS) cells. NDiff N2 contains insulin, APO-transferrin (iron poor), progesterone, putrescine, and sodium selenite. NDiff N2 medium can be used with RHB-Basal medium; this medium is animal component-free and does not contain neuronal supplements, allowing medium customization to meet the specific requirements of your cells.

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Cat. #	Product	Size		License	Quantity	Details
Y40100	NDiff® N2	5 mL	USD $263.00
NDiff N2 is a defined, serum-free cell culture supplement. Based on Bottenstein's formulation, NDiff N2 was originally developed and optimized for the in vitro monolayer differentiation of mouse embryonic stem cells into neurons. The product has subsequently been used to derive, expand and maintain mouse and human NS cells as well as mouse ES cells under serum- and feeder-free culture conditions. NDiff N2 containd insulin, APO-transferrin (iron poor), progesterone, putrescine and sodium selenite. For an animal free alternative, see NDiff N2-AF. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Mouse embryonic stem cells differentiate into neurons when cultured in adherent monoculture with NDiff N2-supplemented basal media Mouse embryonic stem cells differentiate into neurons when cultured in adherent monoculture with NDiff N2-supplemented basal media. The differentiated cells express neuron-specific class III beta-tubulin (TuJ1, green).

Overview

Completely defined, serum-free formulation
Use with RHB-Basal medium for optimal performance

More Information

Components

5 ml (200X) bottle

Applications

These applications require additional basal medium and supplements:

Derivation, maintenance, and expansion of mouse embryonic stem (ES) cells
Neural differentiation of mouse ES cells in monolayer culture
Derivation, maintenance, and expansion of tissue-derived, adherent mouse and human NS cells
Differentiation of mouse and human NS cells into functional neurons

References

Bottenstein J.E., Cell Culture in the Neurosciences. Plenum Press (1985) New York and London.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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