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PrimerArray series for disease pathways

When performing gene expression analysis by real-time PCR, ready-to use primer sets for RT-PCR save time, minimize pipetting, and reduce error. Included in the PrimerArray series are sets of real-time RT-PCR primers for analyzing the expression of genes associated with specific disease states in human (Cat.# PH011–PH015) or mouse (Cat.# PN011–PN015). These PrimerArray RT-PCR primer sets allow efficient workflows for colorectal cancer gene expression analysis as well as other diseases such as pancreatic cancer, prostate cancer, melanoma, small lung cell cancer, non-small lung cell cancer, asthma, rheumatoid arthritis, and Type I or Type II diabetes.

When performing gene expression analysis by real-time PCR, ready-to use primer sets for RT-PCR save time, minimize pipetting, and reduce error. Included in the PrimerArray series are sets of real-time RT-PCR primers for analyzing the expression of genes associated with specific disease states in human (Cat.# PH011–PH015) or mouse (Cat.# PN011–PN015). These PrimerArray RT-PCR primer sets allow efficient workflows for colorectal cancer gene expression analysis as well as other diseases such as pancreatic cancer, prostate cancer, melanoma, small lung cell cancer, non-small lung cell cancer, asthma, rheumatoid arthritis, and Type I or Type II diabetes.

Each array contains 96 primer pairs representing 88 genes associated with a disease pathway and 8 housekeeping genes. When comparing an unknown sample to a control sample, gene expression differences can be measured using the relative quantification method. The expression levels of multiple genes can be screened simultaneously. The PrimerArray Analysis Tool Ver. 2.2, available for download on this web page, and/or Multiplate RQ (for use with the Thermal Cycler Dice Real Time Systems, not available in all geographic locations) are useful for analyzing data obtained using these products. Primer set information and the PrimerArray analysis tool can be downloaded by expanding the Details tab in the product table below and selecting the Resources tab for your product of interest.

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Cat. # Product Size Price License Quantity Details
PH014 PrimerArray® Asthma & Rheumatoid Arthritis (Human) 1 Set $594.00

The PrimerArray Series are sets of real-time RT-PCR primers for the analysis of the expression of genes associated with specific biological pathways. Each array contains 96 primer pairs representing 88 genes associated with a biological pathway and 8 housekeeping genes. When comparing an unknown sample to a control sample, gene expression differences can be expressed using the relative quantification method. In addition, expression levels of multiple genes can be screened simultaneously.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like
PN014 PrimerArray® Asthma & Rheumatoid Arthritis (Mouse) 1 Set $594.00

The PrimerArray Series are sets of real-time RT-PCR primers for the analysis of the expression of genes associated with specific biological pathways. Each array contains 96 primer pairs representing 88 genes associated with a biological pathway and 8 housekeeping genes. When comparing an unknown sample to a control sample, gene expression differences can be expressed using the relative quantification method. In addition, expression levels of multiple genes can be screened simultaneously.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like
PH011 PrimerArray® Colorectal Cancer & Pancreatic Cancer (Human) 1 Set $594.00

The PrimerArray Series are sets of real-time RT-PCR primers for the analysis of the expression of genes associated with specific biological pathways. Each array contains 96 primer pairs representing 88 genes associated with a biological pathway and 8 housekeeping genes. When comparing an unknown sample to a control sample, gene expression differences can be expressed using the relative quantification method. In addition, expression levels of multiple genes can be screened simultaneously.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Example of relative gene expression analysis using the PrimerArray series and PrimerArray Analysis Tool v2

Example of relative gene expression analysis using the PrimerArray series and PrimerArray Analysis Tool v2
Example of relative gene expression analysis using the PrimerArray series and PrimerArray Analysis Tool v2.2. In this 3D Profile, the expression level (Fold Difference) of the unknown sample is normalized to the expression level of the control sample (i.e., control expression set to 1.0).
PN011 PrimerArray® Colorectal Cancer & Pancreatic Cancer (Mouse) 1 Set $594.00

The PrimerArray Series are sets of real-time RT-PCR primers for the analysis of the expression of genes associated with specific biological pathways. Each array contains 96 primer pairs representing 88 genes associated with a biological pathway and 8 housekeeping genes. When comparing an unknown sample to a control sample, gene expression differences can be expressed using the relative quantification method. In addition, expression levels of multiple genes can be screened simultaneously.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like
PH015 PrimerArray® Diabetes Mellitus, Type I & Type II (Human) 1 Set $594.00

The PrimerArray Series are sets of real-time RT-PCR primers for the analysis of the expression of genes associated with specific biological pathways. Each array contains 96 primer pairs representing 88 genes associated with a biological pathway and 8 housekeeping genes. When comparing an unknown sample to a control sample, gene expression differences can be expressed using the relative quantification method. In addition, expression levels of multiple genes can be screened simultaneously.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like
PN015 PrimerArray® Diabetes Mellitus, Type I & Type II (Mouse) 1 Set $594.00

The PrimerArray Series are sets of real-time RT-PCR primers for the analysis of the expression of genes associated with specific biological pathways. Each array contains 96 primer pairs representing 88 genes associated with a biological pathway and 8 housekeeping genes. When comparing an unknown sample to a control sample, gene expression differences can be expressed using the relative quantification method. In addition, expression levels of multiple genes can be screened simultaneously.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like
PH012 PrimerArray® Prostate Cancer & Melanoma (Human) 1 Set $594.00

The PrimerArray Series are sets of real-time RT-PCR primers for the analysis of the expression of genes associated with specific biological pathways. Each array contains 96 primer pairs representing 88 genes associated with a biological pathway and 8 housekeeping genes. When comparing an unknown sample to a control sample, gene expression differences can be expressed using the relative quantification method. In addition, expression levels of multiple genes can be screened simultaneously.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like
PN012 PrimerArray® Prostate Cancer & Melanoma (Mouse) 1 Set $594.00

The PrimerArray Series are sets of real-time RT-PCR primers for the analysis of the expression of genes associated with specific biological pathways. Each array contains 96 primer pairs representing 88 genes associated with a biological pathway and 8 housekeeping genes. When comparing an unknown sample to a control sample, gene expression differences can be expressed using the relative quantification method. In addition, expression levels of multiple genes can be screened simultaneously.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like
PH013 PrimerArray® Small Cell Lung Cancer & Non-small Lung Cancer (Human) 1 Set $594.00

The PrimerArray Series are sets of real-time RT-PCR primers for the analysis of the expression of genes associated with specific biological pathways. Each array contains 96 primer pairs representing 88 genes associated with a biological pathway and 8 housekeeping genes. When comparing an unknown sample to a control sample, gene expression differences can be expressed using the relative quantification method. In addition, expression levels of multiple genes can be screened simultaneously.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like
PN013 PrimerArray® Small Cell Lung Cancer & Non-small Lung Cancer (Mouse) 1 Set $594.00

The PrimerArray Series are sets of real-time RT-PCR primers for the analysis of the expression of genes associated with specific biological pathways. Each array contains 96 primer pairs representing 88 genes associated with a biological pathway and 8 housekeeping genes. When comparing an unknown sample to a control sample, gene expression differences can be expressed using the relative quantification method. In addition, expression levels of multiple genes can be screened simultaneously.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like

Overview

  • Affordable: Each PrimerArray Set supplies sufficient qPCR primers for up to 12 real-time PCR runs (25 µl qPCR reaction volume)

  • Minimizes pipetting and reduces error

  • Enables high-throughput analysis of disease pathway gene expression

  • Optimized for superior performance with TB Green Ex Taq qPCR kits

  • PrimerArray Analysis Tool makes data analysis easy

  • Compatible with all standard real-time PCR instrument systems

More Information

Applications

  • Disease pathway gene expression analysis for major diseases such as colorectal cancer, diabetes, melanoma, prostate cancer, lung cancer, rheumatoid arthritis, and asthma

  • Screening for expression of disease pathways in response to drug targets or conditions

Procedural overview for gene expression analysis by real-time RT-PCR

The overall workflow for the PrimerArray sets is straightforward:

  1. RNA extraction (30 min): We recommend NucleoSpin RNA kits (Cat. #740955.50) or RNAiso (Cat.# 9108).
    (Products not available in all geographic locations.)

  2. Reverse transcription (20 min): We recommend PrimeScript RT Master Mix (Perfect Real Time) (Cat.# RR036A).

  3. Dispense real-time PCR reaction solution (10 min): Prepare a master mix of your cDNA sample and TB Green Premix Ex Taq II (Tli RNase Plus) (Cat.# RR820A) and dispense into a 96-well plate.

  4. Add PrimerArray primers (5 min): Use 4 µl per reaction.

  5. Real-time PCR (1–2 hours): Compatible with all commonly used real-time PCR systems

  6. Data analysis (30 min): PrimerArray Analysis Tool makes data analysis easy.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Capturem Trypsin for a rapid, efficient mass spectometry workflow at room temperature.

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Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion.

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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