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Saline sodium citrate buffer (SSC) powder
Saline Sodium Citrate (SSC) is used as a hybridization buffer or a wash buffer in Southern blotting, in situ hybridization, DNA microarrays, or Northern blotting. These products are powders for preparing SSC buffers; each individual powder-containing pouch yields 1 L of SSC buffer, pH 7.0 (2X or 20X).
- Hybridization or wash buffer in Southern blotting, in situ hybridization, DNA microarrays, or Northern blotting
- Saline Sodium Citrate Buffer (SSC) 2X Powder, pH 7.0 (Cat. #T9171), 5 pouches (for 5 L)
Final solution from one pouch (1 L of 2X SSC, pH 7.0) contains:
- 0.03 M Sodium citrate
- 0.3 M NaCl
- Saline Sodium Citrate Buffer (SSC) 20X Powder, pH 7.0 (Cat. #T9172), 5 pouches (for 5 L)
Final solution from one pouch (1 L of 20X SSC, pH 7.0) contains:
- 0.3 M Sodium citrate
- 3.0 M NaCl
Store powder at room temperature.
Empty the contents of one pouch in a flask or beaker. Add 300 ml of distilled water or deionized water and stir the solution briefly. Adjust the volume to 1,000 ml, and stir until completely dissolved.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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