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Technical notes View Capturem Protein G data
Technical notes Antibody purification overview
Home › Products › Protein research › Purification products › Antibody purification › Capturem Protein G

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Technical notes View Capturem Protein G data
Technical notes Antibody purification overview

Rapid Protein G-based antibody purification

Capturem Protein G

Capturem Protein G columns and plates combine the specific antibody binding properties of Protein G with Capturem technology to provide rapid, high-quality purification and screening of monoclonal and polyclonal antibodies. Where resin columns fall short, Capturem Protein G yields highly pure monoclonal and polyclonal antibodies in just 15 minutes, from loading the sample through elution.

Capturem Protein G columns and plates combine the specific antibody binding properties of Protein G with Capturem technology to provide rapid, high-quality purification and screening of monoclonal and polyclonal antibodies. Where resin columns fall short, Capturem Protein G yields highly pure monoclonal and polyclonal antibodies in just 5–15 minutes, from loading the sample through elution. Short residence times greatly reduce the possibility of antibody aggregation or loss of activity, giving downstream applications a higher probability of success.

Protein G is a cell wall protein from the bacterium Staphylococcus aureus with a high affinity for the Fc region of monoclonal and polyclonal IgG-type antibodies from several different species, e.g., mouse, rat, human, etc. This product uses a recombinant Protein G that was engineered to remove the albumin-binding domain, as well as the cell wall and cell membrane binding domains, in order to improve specificity towards the Fc region of antibodies. Capturem kits use a novel high-capacity membrane-based affinity technology that has previously been used to purify highly concentrated his-tagged proteins in just minutes at room temperature, via miniprep columns, maxiprep columns, 24-well plates, and 96-well plates.

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Cat. # Product Size Price License Quantity Details
635732 Capturem™ Protein G 24-Well Plate 1 x 24-well plate USD $334.00

License Statement

ID Number  
273 This product is covered by U.S. Patent Nos. 9,459,188 and/or 10,207,229, exclusively licensed to Takara Bio USA, Inc.

Capturem Protein G 24-Well Plates are 24-well plates that provide rapid, high-quality, small-scale purification of monoclonal and polyclonal antibodies. It combines the specific antibody-binding properties of Protein G with novel Capturem technology to provide high-capacity, membrane-based affinity purification. Typical yields for monoclonal antibodies are up to 600 µg with a 4.5-ml sample capacity.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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Capturem Protein A- or G-functionalized membranes

Capturem Protein A- or G-functionalized membranes

Antibody purification workflow for Capturem Protein A or G 96-well plates. Each well can be loaded with up to 850 µl of a diluted sample (antibody sample diluted from 1:1 to 1:20 with buffer). Following binding, the membrane is then washed and the bound antibiody eluted with the appropriate buffers. Note: Protocol time varies for each format. Check user manuals for specifics on protocol times.

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Capturem spin columns and filtration devices

Capturem spin columns and filtration devices

Available Capturem spin column and filtration device formats. Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats.

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Load volumes and approximate yields for available Capturem formats by chemistry

Load volumes and approximate yields for available Capturem formats by chemistry

Load volumes and yields of Capturem purification formats.

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635732: Capturem Protein G 24-Well Plate

635732: Capturem Protein G 24-Well Plate
635744 Capturem™ Protein G 24-Well Plate - HC 4 x 24-Well Plate USD $1593.00

License Statement

ID Number  
273 This product is covered by U.S. Patent Nos. 9,459,188 and/or 10,207,229, exclusively licensed to Takara Bio USA, Inc.

The Capturem Protein G 24-Well Plate - HC is a high-capacity, single-use disposable plate for the simple, rapid purification of antibodies. Up to 4.3 ml of sample can be loaded per well. This plate is suitable for purification of antibodies from animal serum, ascites fluid, cell culture supernatant of hybridoma cell lines, and other sources.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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Load volumes and approximate yields for available Capturem formats by chemistry

Load volumes and approximate yields for available Capturem formats by chemistry

Load volumes and yields of Capturem purification formats.

Back

Capturem spin columns and filtration devices

Capturem spin columns and filtration devices

Available Capturem spin column and filtration device formats. Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats.

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Capturem Protein A- or G-functionalized membranes

Capturem Protein A- or G-functionalized membranes

Antibody purification workflow for Capturem Protein A or G 96-well plates. Each well can be loaded with up to 850 µl of a diluted sample (antibody sample diluted from 1:1 to 1:20 with buffer). Following binding, the membrane is then washed and the bound antibiody eluted with the appropriate buffers. Note: Protocol time varies for each format. Check user manuals for specifics on protocol times.

635745 Capturem™ Protein G 24-Well Plate - HC 1 x 24-Well Plate USD $445.00

License Statement

ID Number  
273 This product is covered by U.S. Patent Nos. 9,459,188 and/or 10,207,229, exclusively licensed to Takara Bio USA, Inc.

The Capturem Protein G 24-Well Plate - HC is a high-capacity, single-use disposable plate for the simple, rapid purification of antibodies. Up to 4.3 ml of sample can be loaded per well. This plate is suitable for purification of antibodies from animal serum, ascites fluid, cell culture supernatant of hybridoma cell lines, and other sources.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Load volumes and approximate yields for available Capturem formats by chemistry

Load volumes and approximate yields for available Capturem formats by chemistry

Load volumes and yields of Capturem purification formats.

Back

Capturem spin columns and filtration devices

Capturem spin columns and filtration devices

Available Capturem spin column and filtration device formats. Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats.

Back

Capturem Protein A- or G-functionalized membranes

Capturem Protein A- or G-functionalized membranes

Antibody purification workflow for Capturem Protein A or G 96-well plates. Each well can be loaded with up to 850 µl of a diluted sample (antibody sample diluted from 1:1 to 1:20 with buffer). Following binding, the membrane is then washed and the bound antibiody eluted with the appropriate buffers. Note: Protocol time varies for each format. Check user manuals for specifics on protocol times.

635726 Capturem™ Protein G 96-Well Plate 1 x 96-well plate USD $425.00

License Statement

ID Number  
273 This product is covered by U.S. Patent Nos. 9,459,188 and/or 10,207,229, exclusively licensed to Takara Bio USA, Inc.

Capturem Protein G 96 is a single-use disposable 96-well plate for simple, rapid purification of antibodies that yields up to 60 μg of purified antibody per well, depending upon species and antibody isotype. Capturem Protein G 96 is suitable for purification of antibodies from animal sera, ascites fluid, cell culture media and other sources.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Capturem Protein A- or G-functionalized membranes

Capturem Protein A- or G-functionalized membranes

Antibody purification workflow for Capturem Protein A or G 96-well plates. Each well can be loaded with up to 850 µl of a diluted sample (antibody sample diluted from 1:1 to 1:20 with buffer). Following binding, the membrane is then washed and the bound antibiody eluted with the appropriate buffers. Note: Protocol time varies for each format. Check user manuals for specifics on protocol times.

Back

Capturem spin columns and filtration devices

Capturem spin columns and filtration devices

Available Capturem spin column and filtration device formats. Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats.

Back

Load volumes and approximate yields for available Capturem formats by chemistry

Load volumes and approximate yields for available Capturem formats by chemistry

Load volumes and yields of Capturem purification formats.

Back

635726: Capturem Protein G 96-Well Plate

635726: Capturem Protein G 96-Well Plate
635727 Capturem™ Protein G Maxiprep Columns 6 Columns USD $295.00

License Statement

ID Number  
273 This product is covered by U.S. Patent Nos. 9,459,188 and/or 10,207,229, exclusively licensed to Takara Bio USA, Inc.

Capturem Protein G Maxiprep columns are single-use disposable columns for simple, rapid purification of antibodies that yields up to 1–2 mg of purified antibody per column, depending upon antibody isotype and species. This maxiprep column is suitable for purification of antibodies from animal sera, ascites fluid, cell culture media and other sources.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Capturem Protein A- or G-functionalized membranes

Capturem Protein A- or G-functionalized membranes

Antibody purification workflow for Capturem Protein A or G 96-well plates. Each well can be loaded with up to 850 µl of a diluted sample (antibody sample diluted from 1:1 to 1:20 with buffer). Following binding, the membrane is then washed and the bound antibiody eluted with the appropriate buffers. Note: Protocol time varies for each format. Check user manuals for specifics on protocol times.

Back

Capturem spin columns and filtration devices

Capturem spin columns and filtration devices

Available Capturem spin column and filtration device formats. Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats.

Back

Load volumes and approximate yields for available Capturem formats by chemistry

Load volumes and approximate yields for available Capturem formats by chemistry

Load volumes and yields of Capturem purification formats.

Back

635727: Capturem Protein G Maxiprep Columns

635727: Capturem Protein G Maxiprep Columns
635725 Capturem™ Protein G Miniprep Columns 10 Columns USD $198.00

License Statement

ID Number  
273 This product is covered by U.S. Patent Nos. 9,459,188 and/or 10,207,229, exclusively licensed to Takara Bio USA, Inc.

Capturem Protein G Miniprep consists of single-use disposable columns for simple, rapid purification of antibodies that yield 50–100 μg of purified antibody per column, depending upon the sample type, species, and antibody isotype. These miniprep columns are suitable for purification of antibodies from animal sera, ascites fluid, cell culture media, and other sources.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Capturem Protein A- or G-functionalized membranes

Capturem Protein A- or G-functionalized membranes

Antibody purification workflow for Capturem Protein A or G 96-well plates. Each well can be loaded with up to 850 µl of a diluted sample (antibody sample diluted from 1:1 to 1:20 with buffer). Following binding, the membrane is then washed and the bound antibiody eluted with the appropriate buffers. Note: Protocol time varies for each format. Check user manuals for specifics on protocol times.

Back

Capturem spin columns and filtration devices

Capturem spin columns and filtration devices

Available Capturem spin column and filtration device formats. Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats.

Back

Load volumes and approximate yields for available Capturem formats by chemistry

Load volumes and approximate yields for available Capturem formats by chemistry

Load volumes and yields of Capturem purification formats.

Back

635725: Capturem Protein G Miniprep Columns

635725: Capturem Protein G Miniprep Columns

Overview

  • Fast and convenient: room-temperature protocol, complete in under 15 minutes, without incubation
  • High purity: small column bed volumes trap fewer contaminants and allow large-volume washes
  • High quality: short residence times reduce the possibility of aggregation or loss of activity
  • Flexible: purify a wide range of antibodies

Note: Capturem Protein G 24-Well Plate - HC is optimized to give you an increased yield compared to our previous version.

More Information

Applications

  • Antibody purification
  • Immunoprecipitation

Approximate yields for Capturem Protein G formats

Format Yield* Purification time
Miniprep 60 µg 5 min
96-well plate 60 µg 15 min
24-well plate - HC 600 µg** 15 min
Maxiprep 1.2 mg 15 min

*Yields vary depending upon the sample type, species, and antibody isotype.

**This yield was obtained when spiking 1 mg of mouse IgG1 in HEK293 cell supernatant.

Isotype affinity of Protein A and Protein G

Species Antibody subtype Protein A Protein G Protein A/G
Human

Total IgG

​IgG1

IgG2

​IgG3

​IgG4

​IgM

​IgD

​IgA1

IgA2

Fab

scFv

+++

+++

+++

+

+++

+

−

+

+

+

+

+++

+++

+++

+++

+++

−

−

−

−

+

−

+++

+++

+++

+++

+++

+

−

+

+

+

+

Mouse

Total IgG

IgM

IgG1

IgG2a

​IgG2b

IgG3

+++

−

+

+++

+++

+++

+++

−

++

+++

+++

+++

+++

−

++

+++

+++

+++

Rat

Total IgG

IgG1

IgG2a

IgG2b

IgG2c

+

+

−

−

+++

++

++

+++

+

+++

++

++

+++

+

+++

Rabbit Total IgG +++ +++ +++

Key: +++ strong binding; ++ medium binding; + weak binding; − no binding

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Protein research learning resources

His-tagged protein purification

Choose the right platform for purifying your his-tagged proteins.

Antibody purification

Resources to learn about antibody purification tools including Capturem Protein A columns.

Purification with other tags

FLAG, GST, and Myc tags for protein purification.

Phosphoprotein and glycoprotein purification

Learn more about phosphoprotein and glycoprotein purification.

Expression systems

Choose your protein expression platform.

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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