Rapid Protein G-based antibody purification
Capturem Protein G columns and plates combine the specific antibody binding properties of Protein G with Capturem technology to provide rapid, high-quality purification and screening of monoclonal and polyclonal antibodies. Where resin columns fall short, Capturem Protein G yields highly pure monoclonal and polyclonal antibodies in just 15 minutes, from loading the sample through elution.
Capturem Protein G columns and plates combine the specific antibody binding properties of Protein G with Capturem technology to provide rapid, high-quality purification and screening of monoclonal and polyclonal antibodies. Where resin columns fall short, Capturem Protein G yields highly pure monoclonal and polyclonal antibodies in just 5–15 minutes, from loading the sample through elution. Short residence times greatly reduce the possibility of antibody aggregation or loss of activity, giving downstream applications a higher probability of success.
Protein G is a cell wall protein from the bacterium Staphylococcus aureus with a high affinity for the Fc region of monoclonal and polyclonal IgG-type antibodies from several different species, e.g., mouse, rat, human, etc. This product uses a recombinant Protein G that was engineered to remove the albumin-binding domain, as well as the cell wall and cell membrane binding domains, in order to improve specificity towards the Fc region of antibodies. Capturem kits use a novel high-capacity membrane-based affinity technology that has previously been used to purify highly concentrated his-tagged proteins in just minutes at room temperature, via miniprep columns, maxiprep columns, 24-well plates, and 96-well plates.
Overview
- Fast and convenient: room-temperature protocol, complete in under 15 minutes, without incubation
- High purity: small column bed volumes trap fewer contaminants and allow large-volume washes
- High quality: short residence times reduce the possibility of aggregation or loss of activity
- Flexible: purify a wide range of antibodies
Note: Capturem Protein G 24-Well Plate - HC is optimized to give you an increased yield compared to our previous version.
More Information
Applications
- Antibody purification
- Immunoprecipitation
Approximate yields for Capturem Protein G formats
| Format | Yield* | Purification time |
| Miniprep | 60 µg | 5 min |
| 96-well plate | 60 µg | 15 min |
| 24-well plate - HC | 600 µg** | 15 min |
| Maxiprep | 1.2 mg | 15 min |
*Yields vary depending upon the sample type, species, and antibody isotype.
**This yield was obtained when spiking 1 mg of mouse IgG1 in HEK293 cell supernatant.
Isotype affinity of Protein A and Protein G
| Species | Antibody subtype | Protein A | Protein G | Protein A/G |
| Human |
Total IgG IgG1 IgG2 IgG3 IgG4 IgM IgD IgA1 IgA2 Fab scFv |
+++ +++ +++ + +++ + − + + + + |
+++ +++ +++ +++ +++ − − − − + − |
+++ +++ +++ +++ +++ + − + + + + |
| Mouse |
Total IgG IgM IgG1 IgG2a IgG2b IgG3 |
+++ − + +++ +++ +++ |
+++ − ++ +++ +++ +++ |
+++ − ++ +++ +++ +++ |
| Rat |
Total IgG IgG1 IgG2a IgG2b IgG2c |
+ + − − +++ |
++ ++ +++ + +++ |
++ ++ +++ + +++ |
| Rabbit | Total IgG | +++ | +++ | +++ |
Key: +++ strong binding; ++ medium binding; + weak binding; − no binding
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Protein research learning resources
His-tagged protein purification
Choose the right platform for purifying your his-tagged proteins.
Antibody purification
Resources to learn about antibody purification tools including Capturem Protein A columns.
Purification with other tags
FLAG, GST, and Myc tags for protein purification.
Phosphoprotein and glycoprotein purification
Learn more about phosphoprotein and glycoprotein purification.
Expression systems
Choose your protein expression platform.
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