Protein digestion: arginylendopeptidase

Arginylendopeptidase cleaves peptide bonds at the carboxyl side of arginine residues located on proteins and peptides, enabling protein fragmentation for structural analysis. Arginylendopeptidase is also known as mouse submaxillary protease D or mouse EGF-binding protein C. This enzyme has been treated with TLCK and TPCK to remove trace trypsin-like and chymotrypsin-like protease activities. The product is supplied with a 5X Reaction Buffer [250 mM sodium phosphate buffer (pH 8.0)].

NOTE: This enzyme has weak activity towards -Lys-X- sites, especially when those sites are preceded by a basic amino acid residue.

Cat. #	Product	Size		License	Quantity	Details
7308	Arginylendopeptidase	0.5 mg	USD $189.00
Arginylendopeptidase specifically cleaves the peptide bonds at the carboxyl side of arginine residues of peptides or proteins. This enzyme is also known as mouse submaxillary protease D and as mouse EGF binding protein type C. This product has been treated with TLCK and TPCK to remove trace contamination of trypsin-like and chymotrypsin-like proteases activities after purification. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 7308: Arginylendopeptidase

More Information

Applications

Protein digestion
Fragmentation of proteins and peptides prior to structural analysis

Source

Mouse submaxillary gland

Storage

−20°C

Properties

Molecular weight	21.3 kDa (gel filtration)
Optimum pH	8.0–9.0
Isoelectric point	5.65
Inhibitors	PMSF, DFP
Tolerance to denaturants	Less than or equal to 2 M Urea Less than or equal to 0.1 M Guanidine-HCl Less than or equal to 0.05% SDS

Form

In a solution of 5 mM sodium phosphate buffer (pH 7.2) containing 50% glycerol.

Definition of activity

One unit of activity corresponds to the amount of enzyme required to produce 1 mmol p-nitroaniline from benzoyl-DL-arginine p-nitroanilide (BAPA) in 1 min at 37°C and pH 8.0.

Product Citations

Isackson PJ, Silverman RE, Blaber M, Server AC, Nichols RA, Shooter EM, Bradshaw, R. Epidermal growth factor binding protein: identification of a different protein. Biochemistry 26, 2082-5 (1987).

Matsushita H, Kato I, Aoyama H, Tsunasawa S, S. F. Arginylendopeptidase. Protein, Nucleic Acid Enzym. (Japanese Journal) 34, 374-9 (1989).

Schenkein, I., Levy, M., Franklin, E.C., Frangione, B. Proteolytic enzymes from the mouse submaxillary gland. Specificity restricted to arginine residues. Arch Biochem Biophys. 182, 64-70 (1977).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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