Capturem Pepsin digestion for mass spectrometry
Capturem Pepsin provides fast, efficient, and complete digestion of proteins at room temperature, in comparison to typical protein digestion workflows for mass spectrometry (MS) analyses that require four-hour or overnight digestion. By contrast, Capturem Pepsin's rapid and simple protocol allows for an uninterrupted proteomic analysis workflow. This product, based on our novel Capturem technology, provides membrane-immobilized pepsin in a mini spin column format.
Capturem Pepsin provides fast, efficient, and complete digestion of proteins at room temperature, in comparison to typical protein digestion workflows for mass spectrometry (MS) analyses that require four-hour or overnight digestion. By contrast, Capturem Pepsin's rapid and simple protocol allows for an uninterrupted proteomic analysis workflow. This product, based on our novel Capturem technology, provides membrane-immobilized pepsin in a mini spin column format. Capturem Pepsin completely digests protein samples in just a few minutes, compared to the time-consuming incubations often required for in-solution digestion, and provides better efficiency with fewer autolyzed fragments. In addition, this Capturem spin column makes it easier to avoid the over-digestion commonly seen with in-solution digests.
More effective proteomic analysis
Protein digestion using Capturem Pepsin is more effective than in-solution digestion for preparing samples for MS analysis because it eliminates long digestion times, which may lead to oxidation and other protein modifications, as well as increased autolysis, which can make peptide and protein identification more difficult and less reproducible.
Fast, easy protocol
Capturem Pepsin includes mini spin columns and an activation buffer. Capturem protein digestion is performed using a fast, easy protocol. Just load 200 µl of activation buffer, spin for one minute, then load your protein sample and spin for another minute to elute peptides that are ready for downstream analysis.
Overview
- Digests protein samples in less than two minutes instead of overnight
- Generates fewer autolyzed pepsin fragments and protein modifications
- Allows an uninterrupted proteomics analysis workflow
- Enables more effective peptide and protein identification in MS analysis
More Information
Applications
- Protein characterization
- Proteomic analysis
- Mass spectrometry sample preparation
- Antibody characterization
Additional product information
Please see the product′s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Get the data on fast, efficient protein digestion with Capturem Trypsin and Capturem Pepsin
Capturem Pepsin and Capturem Trypsin were developed for complete digestion of proteins without the need for the long incubation times typically required for in-solution protocols. Both of these kits are powerful tools that allow for an uninterrupted proteomic analysis workflow. The performance of both kits has been demonstrated using apomyoglobin, a common protein mapping standard. Apomyoglobin peptides generated by Capturem Trypsin and Capturem Pepsin digestion were shown to provide good sequence coverage without over-digestion of the samples. Click the buttons below to view technical data on each digestion kit.
Capturem Pepsin Capturem TrypsinTakara Bio USA, Inc.
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