Refolding cycloamylose (CA) kit
The Refolding CA Kit takes advantage of a novel artificial chaperone technology in an easy-to-use two-step process to optimize refolding conditions of inclusion body proteins. Optimization of protein refolding conditions results in correct protein folding and restoration of protein activity. One can also use this kit to solubilize inclusion body proteins.
The Refolding CA (Cycloamylose) Kit takes advantage of a novel artificial chaperone technology in an easy-to-use two-step process to optimize refolding conditions of inclusion body proteins. Optimization of protein refolding conditions results in correct protein folding and restoration of protein activity. One can also use this kit to solubilize inclusion body proteins.
The smaller-size Refolding CA Kit is supplied with guanidine hydrochloride (GdmCl) and dithiothreitol (DTT) for protein unfolding, four different surfactants that can be added independently to the unfolded protein solution (protecting the protein from molecular aggregation), and highly polymerized cycloamylose (CA), an artificial chaperone, for surfactant removal and recovery of protein activity. Overnight incubation of the CA-treated protein is followed by a quick 10-min centrifugation step and collection of the refolded protein-containing supernatant.
The Refolding CA Large Kit is intended for large-scale protein refolding after reaction conditions have been optimized using the smaller Refolding CA Kit. The large kit consists of only denaturant and CA.
The artificial chaperone technology in the Refolding CA Kit is licensed from NFRI, BTRAI and Ezaki Glico Co, Ltd.
- Refolding of isolated inclusion body proteins
- Solubilization of inclusion body proteins
- Recovery of protein activity
Refolding CA Kit (Cat. # 7350)
|8 M GdmCl||2 x 1 ml|
|4 M DTT||50 µl|
|» 1% Tween 40||2 x 1 ml|
|» 1% Tween 60||2 x 1 ml|
|» 1% CTAB (cetyltrimethylammoniumbromide)||2 x 1 ml|
|» 1% SB3-14 (myristylsulfobetaine||2 x 1 ml|
|200 mM DL-cystine||2 x 0.75 ml|
|3% CA (highly polymerized cycloamylose)||7 x 1.6 ml|
|Refolding CA Large Kit (Cat. # 7351)|
|3% CA||6 x 20 ml|
|8 M GdmCl||2 x 10 ml|
Daugherty, D. L., Rozema, D., Hanson, P. E. & Gellman, S. H. Artificial chaperone-assisted refolding of citrate synthase. J. Biol. Chem. 273, 33961–71 (1998).
Machida, S. et al. Cycloamylose as an efficient artificial chaperone for protein refolding. FEBS Lett. 486, 131–5 (2000).
Sivakama Sundari, C., Raman, B. & Balasubramanian, D. Artificial chaperoning of insulin, human carbonic anhydrase and hen egg lysozyme using linear dextrin chains--a sweet route to the native state of globular proteins. FEBS Lett. 443, 215–9 (1999).
Additional product information
Please see the product documents for information about storage conditions, product components, and technical specifications. Certificates of Analysis and User Manuals are located under the Documents tab.
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