- Purification products
- Expression vectors & systems
- Antibodies and immunoprecipitation
- Mass spectrometry reagents
- Protein sequencing
- Two-hybrid and one-hybrid systems
- SDS-PAGE & western blotting
- Accessory enzymes
Protein detection using his-tag antibodies and an HA tag antibody
The his-tag antibodies and myc-tag antibody detect his-tagged and myc-tag recombinant proteins, respectively, in Western blot, ELISA, and immunocytohistochemical assays. They are highly sensitive and specific for proteins bearing the histidine tag that they were raised against.
The his-tag antibodies detect his-tagged recombinant proteins in Western blot, ELISA, and immunocytohistochemical assays. They are highly sensitive and specific for proteins bearing the histidine tag that they were raised against.
6xHis Monoclonal Antibody (Albumin Free)
This antibody is an IgG2a isotype from mouse ascites fluid. Because this antibody is albumin-free, it provides a high signal-to-noise ratio and can detect as little as 1 ng of 6xhis-tagged protein. It comes in a salt-free form for added stability. Antibody-protein complexes can be visualized using any labeled secondary anti-mouse antibody.
6xHis mAb-HRP Conjugate
This is the same antibody as above but conjugated to horseradish peroxidase (HRP). The HRP conjugate can be used to detect and visualize 6xhis-tagged proteins using chemiluminescent, colorimetric, or fluorometric substrates without requiring a secondary antibody.
6xHN Polyclonal Antibody
An albumin-free rabbit polyclonal antibody for the detection of 6xHN-tagged recombinant protein is available. The antibody can be used for Western blotting and ELISA applications.
c-Myc Monoclonal Antibody
The c-Myc monoclonal mouse antibody recognizes an epitope located within residues 410–419 of the human p62-c-Myc protein. This antibody is suitable for immunoprecipitation of c-Myc-tagged fusion proteins from cell lysates, ELISA, immunocytochemistry of microinjected or transfected cells, and localization of c-Myc-tagged proteins.
- 6xhis monoclonal antibody: detects as little as 1 ng of 6xhis-tagged proteins
- 6xhis monoclonal antibody-HRP conjugate: has high sensitivity and detection does not require a 2° antibody
- 6xHN polyclonal antibody: detects the 6xHN tag and can be used for Western blotting and ELISA
- Western Blot
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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