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Other PCR-related products

Full-length Taq DNA polymerase & PCR kits

The products listed on this page are being phased out. Replacement products are already available:

We recommend replacing Taq full DNA polymerase with the newer TaKaRa Taq DNA polymerase, which is a recombinant version of the thermostable, full-length Taq. Takara Taq is available as individual components, in a hot-start formulation, or as a complete kit.

The products listed on this page are being phased out. Replacement products are already available:

We recommend replacing Taq full DNA polymerase with the newer TaKaRa Taq DNA polymerase, which is a recombinant version of the thermostable, full-length Taq. Takara Taq polymerase is available as individual components, in a hot-start formulation, or as a complete kit.

Taq full DNA polymerase is an ultra-pure, highly efficient, full-length, recombinant version of the Thermus aquaticus (Taq) YT1 DNA Polymerase (94 kD) (Engelke et al. 1990). This enzyme possesses two important catalytic activities: a 5'→3' polymerase activity, and a double-strand-specific 5'→3' exonuclease activity. Like other full-length Taq DNA Polymerases, this enzyme lacks 3'→5' exonuclease (proofreading) activity. This enzyme is suitable for any general PCR amplification procedure. It amplifies even rare targets from bacterial and plasmid DNA, cDNA, and complex genomic DNA.

Taq full DNA polymerase is available in several formats:

Individual components of polymerase enzyme mix (Cat. # 639233 and 639234)—separate tubes of Taq full DNA polymerase and 10X buffer
Taq full DNA polymerase in a complete kit (Cat. # 639235)—includes Taq, 10X buffer, MgCl₂, dNTPs, control genomic DNA template, and primer mix
A hot-start polymerase formulation in a complete kit (Cat. # 639231)—includes hot-start Taq, 10X buffer, MgCl₂, dNTPs, control genomic DNA template, and primer mix

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Cat. #	Product	Size
639234	Taq Full DNA Polymerase Enzyme Mix	5,000 Units	USD $1656.00
The Taq Full DNA Polymerase is a full-length recombinant version of the Thermus aquaticus (Taq) strain YT1 DNA polymerase. It is a broad range enzyme suitable for a variety of general PCR applications. One unit of enzyme incorporates 10 nmols of dNTPs into acid-insoluble material within 30 min at 74°C. The Taq Full DNA Polymerase Enzyme Mix contains the Taq Full DNA Polymerase as well as optimized PCR Buffer containing 20 mM MgCl₂. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Amplification of a single-copy gene from calf thymus genomic DNA using Taq Full DNA Polymerases *Amplification of a single-copy gene from calf thymus genomic DNA using Taq* Full DNA Polymerases.** The gene encoding bovine pancreatic trypsin inhibitor (BPTI) was amplified from the following amounts of calf thymus genomic DNA template: 0 ng (Lane 1), 1 ng (Lane 2), 5 ng (Lane 3), 10 ng (Lane 4), 20 ng (Lane 5), 100 ng (Lane 6), 200 ng (Lane 7), Lane M = DNA size markers; λ-Hind III and FX174-Hae III digests, mixed 1:1. Reactions (50 μl) were performed using 1 unit/reaction of Taq Full DNA Polymerase or Taq Full Hot Start DNA Polymerase. Back Use less enzyme and less template per reaction with Taq Full DNA Polymerase *Use less enzyme and less template per reaction with Taq* Full DNA Polymerase.** A 500 bp portion of the BPTI gene was amplified from 10 ng calf thymus genomic DNA using decreasing amounts (U/rxn) of Taq. Full DNA Polymerase (Panel A) and Taq Full Hot Start DNA Polymerase (Panel B): 5.0 U (Lane 1), 2.5 U (Lane 2), 1.0 U (Lane 3), 0.5 U (Lane 4), 0.2 U (Lane 5), and no enzyme (Lane 6). M = DNA size marker; λ-HindIII and φ174-HaeIII digests, mixed 1:1.. Back Taq Full Hot Start DNA polymerase provides higher yields and more consistent results than similar hot start enzymes Taq Full Hot Start DNA polymerase provides higher yields and more consistent results than similar hot start enzymes. Targets of different sizes were amplified from 100 ng of calf thymus genomic DNA following the manufacturer’s recommendations for each enzyme. [Each 50 μl reaction contained 2 units of enzyme, 1X PCR reaction buffer with the appropriate amount of MgCl₂ for each enzyme, and 400 nM of each primer.] Reactions were assembled at room temperature. Taq Full Hot Start/Buffer B reactions contain 3 mM MgCl₂. These reactions produced amplicons of the following sizes: 500 bp (Lane 1), 900 bp (Lane 2), 2,000 bp (Lane 3), 2,500 bp (Lane 4), 3,500 bp (Lane 5); M = DNA size marker; λ-HindIII and φ174-HaeIII digests, mixed 1:1.
639231	Taq Full Hot Start DNA Polymerase PCR Kit	100 Rxns	USD $593.00
The Taq Full Hot Start DNA Polymerase Enzyme Mix is a mixture consisting of Taq Full DNA Polymerase and TaqStart Antibody, a monoclonal antibody that inhibits Taq Full at ambient temperatures. The Taq Full DNA Polymerase is a full-length recombinant version of the Thermus aquaticus (Taq) strain YT1 DNA polymerase. It is a broad-ranging enzyme suitable for a variety of general PCR applications. One unit of enzyme incorporates 10 nmols of dNTPs into acid-insoluble material within 30 min at 74°C. Each kit contains enzyme mix, optimized PCR buffer containing 20 mM MgCl2, dNTPs, and additional MgCl₂ for 100 PCR reactions (100 units) of 50 μl each. Control Genomic DNA (calf thymus) and Primer Mix are also provided for amplifying a 407-bp control amplicon within the bovine pancreatic trypsin inhibitor gene. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Amplification of a single-copy gene from calf thymus genomic DNA using Taq Full DNA Polymerases *Amplification of a single-copy gene from calf thymus genomic DNA using Taq* Full DNA Polymerases.** The gene encoding bovine pancreatic trypsin inhibitor (BPTI) was amplified from the following amounts of calf thymus genomic DNA template: 0 ng (Lane 1), 1 ng (Lane 2), 5 ng (Lane 3), 10 ng (Lane 4), 20 ng (Lane 5), 100 ng (Lane 6), 200 ng (Lane 7), Lane M = DNA size markers; λ-Hind III and FX174-Hae III digests, mixed 1:1. Reactions (50 μl) were performed using 1 unit/reaction of Taq Full DNA Polymerase or Taq Full Hot Start DNA Polymerase. Back Use less enzyme and less template per reaction with Taq Full DNA Polymerase *Use less enzyme and less template per reaction with Taq* Full DNA Polymerase.** A 500 bp portion of the BPTI gene was amplified from 10 ng calf thymus genomic DNA using decreasing amounts (U/rxn) of Taq. Full DNA Polymerase (Panel A) and Taq Full Hot Start DNA Polymerase (Panel B): 5.0 U (Lane 1), 2.5 U (Lane 2), 1.0 U (Lane 3), 0.5 U (Lane 4), 0.2 U (Lane 5), and no enzyme (Lane 6). M = DNA size marker; λ-HindIII and φ174-HaeIII digests, mixed 1:1.. Back Taq Full Hot Start DNA polymerase provides higher yields and more consistent results than similar hot start enzymes Taq Full Hot Start DNA polymerase provides higher yields and more consistent results than similar hot start enzymes. Targets of different sizes were amplified from 100 ng of calf thymus genomic DNA following the manufacturer’s recommendations for each enzyme. [Each 50 μl reaction contained 2 units of enzyme, 1X PCR reaction buffer with the appropriate amount of MgCl₂ for each enzyme, and 400 nM of each primer.] Reactions were assembled at room temperature. Taq Full Hot Start/Buffer B reactions contain 3 mM MgCl₂. These reactions produced amplicons of the following sizes: 500 bp (Lane 1), 900 bp (Lane 2), 2,000 bp (Lane 3), 2,500 bp (Lane 4), 3,500 bp (Lane 5); M = DNA size marker; λ-HindIII and φ174-HaeIII digests, mixed 1:1.

Overview

Provides superior sensitivity and yield compared to other full-length Taq polymerases
Cost effective—requires less enzyme and template per reaction
Available with hot-start antibody—increases PCR specificity by reducing mispriming events

More Information

References

Engelke, D. R., Krikos, A., Bruck, M. E. & Ginsburg, D. Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli. Anal. Biochem. 191, 396–400 (1990).

Kellogg, D. E. et al. TaqStart Antibody: "hot start" PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase. Biotechniques 16, 1134–7 (1994).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Find answers to your PCR questions

Primer design

Frequently asked questions about primer design for successful PCR.

Optimization

Frequently asked questions about PCR optimization.

Troubleshooting

Frequently asked questions about troubleshooting your PCR problems.

Applications and conditions

Frequently asked questions about general and specific applications for PCR and which polymerases to choose.

Shipping, storage, and handling

Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.

Avoid DNA contamination in PCR

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.

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