Full-length Taq DNA polymerase & PCR kits
The products listed on this page are being phased out. Replacement products are already available:
We recommend replacing Taq full DNA polymerase with the newer TaKaRa Taq DNA polymerase, which is a recombinant version of the thermostable, full-length Taq. Takara Taq is available as individual components, in a hot-start formulation, or as a complete kit.
The products listed on this page are being phased out. Replacement products are already available:
We recommend replacing Taq full DNA polymerase with the newer TaKaRa Taq DNA polymerase, which is a recombinant version of the thermostable, full-length Taq. Takara Taq polymerase is available as individual components, in a hot-start formulation, or as a complete kit.
Taq full DNA polymerase is an ultra-pure, highly efficient, full-length, recombinant version of the Thermus aquaticus (Taq) YT1 DNA Polymerase (94 kD) (Engelke et al. 1990). This enzyme possesses two important catalytic activities: a 5'→3' polymerase activity, and a double-strand-specific 5'→3' exonuclease activity. Like other full-length Taq DNA Polymerases, this enzyme lacks 3'→5' exonuclease (proofreading) activity. This enzyme is suitable for any general PCR amplification procedure. It amplifies even rare targets from bacterial and plasmid DNA, cDNA, and complex genomic DNA.
Taq full DNA polymerase is available in several formats:
- Individual components of polymerase enzyme mix (Cat. # 639233 and 639234)—separate tubes of Taq full DNA polymerase and 10X buffer
- Taq full DNA polymerase in a complete kit (Cat. # 639235)—includes Taq, 10X buffer, MgCl2, dNTPs, control genomic DNA template, and primer mix
- A hot-start polymerase formulation in a complete kit (Cat. # 639231)—includes hot-start Taq, 10X buffer, MgCl2, dNTPs, control genomic DNA template, and primer mix
Overview
- Provides superior sensitivity and yield compared to other full-length Taq polymerases
- Cost effective—requires less enzyme and template per reaction
- Available with hot-start antibody—increases PCR specificity by reducing mispriming events
More Information
References
Engelke, D. R., Krikos, A., Bruck, M. E. & Ginsburg, D. Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli. Anal. Biochem. 191, 396–400 (1990).
Kellogg, D. E. et al. TaqStart Antibody: "hot start" PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase. Biotechniques 16, 1134–7 (1994).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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