Advantage GC cDNA PCR kits

The products listed on this page are being phased out. Replacement products are already available.

We recommend replacing Advantage GC cDNA PCR polymerase with the newer Advantage GC 2 polymerase, which is specially formulated to amplify GC-rich templates (up to 90% GC content).

Advantage GC cDNA PCR Polymerase Mix is an enzyme blend consisting of an N-terminal deletion mutant of Taq DNA polymerase (Barnes, 1992; Barnes, 1994), a proofreading enzyme, and TaqStart Antibody (Kellogg, 1994) for automatic hot-start PCR. Benefits over native Taq DNA polymerase include greater efficiency and sensitivity, amplification of longer products, built-in hot start capability, and increased fidelity. The Advantage GC cDNA PCR Kit comes with the polymerase mix, an optimized buffer, dNTPs, control primers, and template.

Advantage GC kits combine the ability to amplify GC-rich sequences (up to 90% GC) with the established benefits of Advantage PCR. The included optimized buffer contains DMSO and a new reagent, GC-Melt, to facilitate PCR amplification of virtually all GC-rich sequences that are difficult or impossible to amplify by conventional methods.

Our Advantage products have been optimized to produce high yields and long reads. Compared to regular Taq polymerase, Advantage polymerase mix provides more efficient amplification over a wide range of template concentrations. In addition, the optimal range of Mg²⁺ concentration is broader for Advantage polymerase mix than it is for most other enzymes, eliminating the need to spend time optimizing reaction conditions.