Advantage GC cDNA PCR kits
The products listed on this page are being phased out. Replacement products are already available.
We recommend replacing Advantage GC cDNA PCR polymerase with the newer Advantage GC 2 polymerase, which is specially formulated to amplify GC-rich templates (up to 90% GC content).
The products listed on this page are being phased out. Replacement products are already available.
We recommend replacing Advantage GC cDNA PCR polymerase with the newer Advantage GC 2 polymerase, which is specially formulated to amplify GC-rich templates (up to 90% GC content).
Advantage GC cDNA PCR Polymerase Mix is an enzyme blend consisting of an N-terminal deletion mutant of Taq DNA polymerase (Barnes, 1992; Barnes, 1994), a proofreading enzyme, and TaqStart Antibody (Kellogg, 1994) for automatic hot-start PCR. Benefits over native Taq DNA polymerase include greater efficiency and sensitivity, amplification of longer products, built-in hot start capability, and increased fidelity. The Advantage GC cDNA PCR Kit comes with the polymerase mix, an optimized buffer, dNTPs, control primers, and template.
Advantage GC kits combine the ability to amplify GC-rich sequences (up to 90% GC) with the established benefits of Advantage PCR. The included optimized buffer contains DMSO and a new reagent, GC-Melt, to facilitate PCR amplification of virtually all GC-rich sequences that are difficult or impossible to amplify by conventional methods.
Our Advantage products have been optimized to produce high yields and long reads. Compared to regular Taq polymerase, Advantage polymerase mix provides more efficient amplification over a wide range of template concentrations. In addition, the optimal range of Mg2+ concentration is broader for Advantage polymerase mix than it is for most other enzymes, eliminating the need to spend time optimizing reaction conditions.
Overview
- Exhibits high efficiency and sensitivity
- Amplifies up to 5 kb from genomic DNA and up to 10 kb from cDNA
- Built-in hot start for higher specificity and lower background
- Optimized for applications involving cDNA
More Information
Applications
- cDNA PCR
- Long-and-accurate PCR
- RACE
- Hot-start PCR
- GC-rich PCR
- High-fidelity PCR
References
Barnes, W. M. The fidelity of Taq polymerase catalyzing PCR is improved by an N-terminal deletion. Gene 112, 29–35 (1992).
Barnes, W. M. PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates. Proc. Natl. Acad. Sci. U. S. A. 91, 2216–2220 (1994).
Kellogg, D.E., et al. TaqStart Antibody: "hot start" PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase. BioTechniques 16:1134–1137 (1994).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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