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Citations Citations: Titanium Taq
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Citations Citations: Titanium Taq
Titanium Taq, research-use Titanium Taq, research use
Tips and FAQs PCR FAQs

Titanium Taq SP—high-yield DNA polymerase for laboratory testing (GPR-registered)

MDx

Titanium Taq SP DNA Polymerase is a registered general purpose reagent (GPR) appropriate for use in general laboratory applications, including molecular diagnostic development and testing. Titanium Taq SP contains the same Titanium Taq DNA Polymerase and TaqStart Antibody as our standard Titanium Taq products, but with a modified, license-free buffer formulation. Titanium Taq SP performs similarly to our standard Titanium Taq in all tested customer applications. It is ideal for PCR amplification of most DNA templates, and for complex genomic DNA in particular.

Titanium Taq SP DNA Polymerase is a registered general purpose reagent (GPR) appropriate for use in general laboratory applications, including molecular diagnostic development and testing. Titanium Taq SP contains the same Titanium Taq DNA Polymerase and TaqStart Antibody as our standard Titanium Taq products, but with a modified, license-free buffer formulation. Titanium Taq SP performs similarly to our standard Titanium Taq in all tested customer applications. It is ideal for PCR amplification of most DNA templates, and for complex genomic DNA in particular.

Titanium Taq is more robust than wild-type Taq polymerases and allows the amplification of highly complex DNA mixes due to the absence of 5'-exonuclease activity found in wild-type Taq. This novel enzyme contains carefully engineered amino acid substitutions that increase its solubility, making it incredibly sensitive.

Commercial Use of Titanium Taq SP may provide cost-savings on royalty and license fees in some situations. If you are interested in Commercial Use of our products, including OEM, please contact Corporate Development at bd_oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.

If you are purchasing this polymerase for research use only, please visit our standard Titanium Taq DNA Polymerase product page.

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Cat. # Product Size License Quantity Details
639292 Titanium® Taq SP DNA Polymerase 100 Rxns USD $175.00

Titanium Taq SP DNA Polymerase contains the same hot-start, high-yield Titanium Taq DNA polymerase and TaqStart Antibody as the standard Titanium Taq (Cat. # 639208). Cat. # 639292 is a registered general purpose reagent (GPR) appropriate for use in general laboratory applications, including molecular diagnostic development and testing.

Notice to purchaser

This product is a general purpose reagent intended For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated. This product is not intended for a specific application or made for any particular clinical use. The performance characteristics of this product have not been fully established. It is the user’s responsibility to validate the performance of the product, and any component thereof, for any particular use. Resale or transfer of this product, any component thereof, or any substance produced through use of this product, or any component thereof, to any third party is expressly forbidden. To obtain additional rights, please contact your local office or go to our website for additional information.

 

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Titanium Taq efficiently amplifies specific genes from genomic DNA

Titanium Taq efficiently amplifies specific genes from genomic DNA
Titanium Taq efficiently amplifies specific genes from genomic DNA. Human cardiac beta-myosin heavy chain fragments of different lengths were amplified from 100 ng of genomic DNA using Titanium Taq and two leading competitor’s hot start Taq polymerases. Optimal conditions were used for each enzyme, as specified by the manufacturer. Lane 1: 0.5 kb fragment. Lane 2: 1 kb fragment. Lane 3: 1.8 kb fragment. Lane M: 1 kb DNA size ladder.

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TITANIUM Taq is active over a wide range of Mg2+ concentrations

TITANIUM Taq is active over a wide range of Mg2+ concentrations
TITANIUM Taq is active over a wide range of Mg2+ concentrations. TITANIUM was used to amplify a 500 bp region of Calf Thymus genomic DNA. The MgCl2 concentration was varied as indicated. The enzyme performed consistently through the range of Mg2+ used. Lane M: DNA size marker.

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639292: Titanium Taq SP DNA Polymerase

639292: Titanium Taq SP DNA Polymerase

Primer design FAQs Primer design FAQs
Optimization FAQs Optimization FAQs
Troubleshooting FAQs Troubleshooting FAQs

Overview

  • Manufactured and labeled in accordance with requirements for general purpose reagents
  • Functionally tested to help ensure remarkable lot-to-lot reproducibility, consistency, and a wide dynamic range
  • Amplify your target using fewer PCR cycles, thus reducing background
  • Perform PCR without optimizing your reaction conditions—Titanium Taq tolerates a wide range of Mg2+ concentrations
  • Amplify targets of up to 2 kb from highly complex templates, such as mammalian genomic DNA
  • Amplifies rare or low-copy targets
  • Set up your PCR at room temperature and see increased specificity and yield due to the integrated hot-start antibody
  • Bring high performance to commercial and OEM use with cost-savings on royalty and license fees in some situations. (Contact Corporate Development at bd_oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.)

More Information

Applications

  • Genotyping
  • Whole-genome PCR
  • Multiplex PCR
  • Primer extension
  • Hot-start PCR
  • Preparative PCR
  • Rare-template amplification

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Tech notes

Titanium Taq outperforms other polymerases in high-throughput genotyping

Titanium Taq DNA Polymerase is unrivaled for multiplex PCR in high-throughput genotyping applications.

Learn more about whole-genome SNP genotyping with Titanium Taq


pcr success PCR learning center
Citations Citations: Titanium Taq

Find answers to your PCR questions

Primer design

Frequently asked questions about primer design for successful PCR.

Optimization

Frequently asked questions about PCR optimization.

Troubleshooting

Frequently asked questions about troubleshooting your PCR problems.

Applications and conditions

Frequently asked questions about general and specific applications for PCR and which polymerases to choose.

Shipping, storage, and handling

Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.

Avoid DNA contamination in PCR

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.

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