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PCR Human Papillomavirus Detection Set

This primer set is designed for detecting human papillomavirus (HPV), specifically viral DNA of HPV16, 18, and 33. These HPV types have all been detected in cervical carcinoma. Included in this set are specific amplification primers for the E6 region of HPV16, 18, and 33, and control templates. Amplification product sizes are 140 bp for HPV16 and 18, and 141 bp for HPV33. HPV type-specific probes for subsequent hybridization are also provided. The PCR Human Papillomavirus Detection Set works very efficiently when used in conjunction with TaKaRa Taq DNA Polymerase.

Cat. #	Product	Size	Price	License	Quantity	Details
6602	PCR Human Papillomavirus Detection Set	50 Rxns	$319.00
This primer set allows simple and sensitive detection of HPV 16, 18, or 33 by PCR. Included in this set are the specific primers for amplification of the sequence containing E6 region of HPV 16, 18, and 33 (140 bp for HPV 16, 18 and 141 bp for HPV 33) and the HPV type-specific probes for the subsequent hybridization. PCR reagents need to be purchased separately. This primer set will work most efficiently when used in conjunction with Takara Taq DNA polymerase. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Resources

More Information

Applications

Human papillomavirus (HPV) detection
HPV PCR

Control templates

The PCR Human Papillomavirus Detection Set includes three control templates to verify the presence of PCR-amplified DNA fragments. Amplification reactions using control templates HPVT16, HPVT18, and HPVT33, and primer pairs HPVpF/HPVp16R, HPVpF/HPVp18R and HPVpF/HPVp33R, respectively, yield 70-bp DNA fragments. Because the control templates contain sequences complementary to the probes provided in the PCR Human Papillomavirus Detection Set, they can be used as positive controls for each HPV type via dot hybridization.

Note

This product is for research use only.

Product citations

Shimada, M. et al. Amplification and specific detection of transforming gene region of human papillomavirus 16, 18 and 33 in cervical carcinoma by means of the polymerase chain reaction. Jpn. J. Cancer Res. 81, 1–5 (1990).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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