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Magnetic bead-based isolation of genomic DNA from forensic samples—NucleoMag 96 Trace
NucleoMag 96 Trace is designed for the purification of DNA from forensic samples (e.g., buccal swabs, dried blood spots, cigarette filters). After lysis of the sample, genomic DNA is selectively bound to the NucleoMag Beads. Contaminants are washed away and the DNA can be eluted from the beads under low-salt conditions.
NucleoMag 96 Trace is designed for the purification of DNA from forensic samples (e.g., buccal swabs, dried blood spots, cigarette filters). After lysis of the sample, genomic DNA is selectively bound to the NucleoMag Beads. Contaminants are washed away and the DNA can be eluted from the beads under low-salt conditions. Since the magnetic bead technology provides a closed system (no transfer of sample from one well to another is necessary during the preparation), the risk of cross-contamination is reduced. NucleoMag technology can be easily adapted to common laboratory automation platforms. It can be used either with static-pin separators (e.g., NucleoMag SEP) or magnetic separators integrated into robotic workstations.
Typically, 96 samples can be purified in less than 120 minutes on common automation platforms. The kit typically yields 1–3 µg of genomic DNA in an elution volume of >50 µl at a concentration of 10–30 ng/µl. The purified DNA is ready to use for PCR, Southern blotting, and any enzymatic reactions.
- One-tube procedure minimizes risk of cross-contamination
- Small elution volumes (>50 µl) are possible
- Easily adapted to automated use
|Technology||Magnetic bead technology|
|Format||Highly reactive superparamagnetic beads (size of beads: 1–3 µm)|
|Processing||Manual or automated|
|Starting material||Buccal swabs, dried blood spots, cigarette filters|
|Typical yield||1–3 µg|
|Typical concentration||10–30 ng/µl|
|Elution volume||>50 µl|
|Preparation time||<120 min/96 preps|
|Binding capacity||0.4 µg/1 µl beads|
Manual or automated isolation of DNA from:
- Buccal swabs
- Dried blood spots
- Cigarette filters
Purified DNA is ready for:
- STR analysis
- Enzymatic reactions
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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