NucleoSpin FFPE DNA—kit for isolation and purification of DNA from formalin-fixed, paraffin-embedded tissue sections
The NucleoSpin FFPE DNA kit provides a convenient, reliable, and fast method to isolate DNA from formalin-fixed, paraffin-embedded (FFPE) tissue specimens. The procedure omits the use of flammable and malodorous xylene or d-limonene commonly used for deparaffinization. Furthermore, the procedure omits the difficult removal of organic solvent from often barely visible tissue pellets.
The NucleoSpin FFPE DNA kit provides a convenient, reliable, and fast method to isolate DNA from formalin-fixed, paraffin-embedded (FFPE) tissue specimens. The procedure omits the use of flammable and malodorous xylene or d-limonene commonly used for deparaffinization. Further, the procedure omits the difficult removal of organic solvent from often barely visible tissue pellets. NucleoSpin FFPE DNA employs the odorless Paraffin Dissolver (patent pending) and allows efficient lysis in a convenient two-phase system.*
First, the paraffin of FFPE sections is dissolved in the Paraffin Dissolver. Tissue is then digested by Proteinase K to solubilize the fixed tissue and release DNA into solution. Subsequently, heat incubation with a specially designed buffer effectively eliminates crosslinks from the previously released DNA. After addition of ethanol, the lysate is applied to the NucleoSpin FFPE DNA Column and DNA is bound to the silica membrane. Two washing steps help to remove salts, metabolites, and macromolecular cellular components. Pure DNA is finally eluted under low ionic strength conditions in a small volume (5–30 µl) of Elution Buffer BE, yielding highly concentrated DNA.
Isolated DNA is high in concentration, formaldehyde crosslinks are reversed efficiently, and the DNA quality of the sample is well preserved during the complete process of deparaffinization, de-crosslinking, and purification. Thus, isolated DNA shows good performance in typical downstream applications such as PCR.
*Users preferring xylene deparaffinization will find protocols utilizing xylene for deparaffinization in the manual. Deparaffinization using xylene shows the same efficiency as deparaffinization using Paraffin Dissolver. Xylene is not included in the kit.
- Very easy paraffin removal—Paraffin Dissolver (patent pending) included
- Concentrate DNA by elution in small volumes
- Overcome formalin crosslinking of DNA by incubation in de-crosslinking buffer
- High-quality DNA provides improved performance in PCR
|Format||Mini spin columns - XS design|
|Starting material||<7 sections (10 µm) of 250 mm2 total area
<15 mg paraffin**
|Typical yield||Depends on amount and quality of the sample|
|DNA quality||Strongly depends on sample quality|
|Elution volume||5–30 µl|
|Preparation time||~70 min/6 preps|
|Binding capacity||110 µg|
**When using the standard protocol with Paraffin Disolver. Larger quantities of paraffin can be processed when using additional Paraffin Dissolver or the standard protocol with xylene for deparaffinization.
- Rapid isolation of DNA from formalin-fixed, paraffin-embedded samples
- Isolation of DNA from fresh and archived FFPE samples
- Isolation of DNA from specimen on object slides
- Typical downstream application: PCR
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Designed for extra small samples
Purify DNA from small samples such as microdissected tissue, small blood samples, or small amounts of cells. Based on reliable silica-membrane technology, NucleoSpin Tissue XS overcomes the limitations of conventional kits using an innovative new column design with a funnel-shaped ring.Learn more All genomic DNA kits
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