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NucleoMag PCR kits are magnetic-bead-based kits for parallel purification of PCR products, which provide a reliable, cost-efficient, and labor-efficient tool. PCR products are selectively bound to NucleoMag PCR beads, while contaminants (e.g., primers, dNTPs, enzymes, salts) are washed away. Purified PCR products are eluted from the beads under low-salt conditions and are ready to use in downstream applications such as sequencing or microarray spotting.
NucleoMag PCR kits are magnetic-bead-based kits for parallel purification of PCR products, which provide a reliable, cost-efficient, and labor-efficient tool. PCR products are selectively bound to NucleoMag PCR beads, while contaminants (e.g., primers, dNTPs, enzymes, salts) are washed away. Purified PCR products are eluted from the beads under low-salt conditions and are ready to use in downstream applications such as sequencing or microarray spotting. Since the magnetic bead technology provides a closed system (no transfer of sample from one well to another is necessary during the purification procedure), there is no risk of cross-contamination. NucleoMag technology can easily be adapted to common laboratory automation platforms. It can be used either with static-pin separators (e.g., NucleoMag SEP) or magnetic separators integrated into robotic workstations.
- Magnetic-bead-based PCR cleanup
- Closed system—reduced risk of cross-contamination
- Small elution volumes (>25 μl)
- Recovery does not depend on elution volume
- Easily adapted to automation
|Technology||Magnetic bead technology|
|Format||Highly reactive superparamagnetic beads
(size of beads: 1–3 µm)
|Processing||Manual or automated|
|Starting material||<50 µl PCR reaction|
|Fragment size||150 bp–10 kb|
|Recovery volume||25 µl|
|Preparation time||30–45 min/plate|
- Manual or automated PCR cleanup
- Typical downstream application: sequencing
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
DNA cleanup: two applications in one kit
The NucleoSpin Gel and PCR Clean-Up procedure is the easiest way to purify DNA fragments from agarose gels or PCR reactions. The kit includes one buffer for both applications, which contains a pH indicator that displays the correct pH for optimal kit performance. The purification procedure for samples from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities.Learn more Other articles
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