Differential expression analysis on the ICELL8 Single-Cell System

The ICELL8 Chip and Reagent 3' DE Kit enables Illumina sequencing and differential gene expression analysis—via transcript 3' end counting—of single cells isolated on the ICELL8 Single-Cell System (Cat. No. 640000). Following staining, dilution, and dispensing of cells into the 5,184-nanowell ICELL8 3′ DE Chip, CellSelect Software is used to automatically identify wells containing single, viable cells, and to direct nanowell-specific delivery of coupled RT-PCR reagents provided in the kit—including a 5' SMART primer. cDNA synthesis is oligo(dT)-primed using uniquely barcoded primers that have been preprinted into each nanowell of the ICELL8 3′ DE Chip, and PCR amplification of the barcoded cDNA is then performed in-chip. Full-length amplicons are pooled and then purified off-chip, and the sequencing libraries are prepared using a transposase-based method that enriches for cDNA derived from mRNA 3' ends followed by a limited-cycle PCR amplification step that results in incorporation of Illumina index sequences. Most importantly, the kit allows for efficient, cost-effective, and high-throughput single-cell gene expression analysis.

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640164: ICELL8 Chip and Reagent 3' DE Kit

The ICELL8 Chip and Reagent 3' DE Kit enables Illumina sequencing and differential gene expression analysis—via transcript 3' end counting—of single cells isolated on the ICELL8 Single-Cell System. Following staining, dilution, and dispensation of cells into the 5,184-nanowell ICELL8 Chip v2, CellSelect software is used to automatically identify wells containing single, viable cells, and to direct nanowell-specific delivery of coupled RT-PCR reagents provided in the kit-including a 5' SMART primer. cDNA synthesis is oligo(dT)-primed using uniquely barcoded primers that have been preprinted into each nanowell of the ICELL8 Chip v2, and PCR amplification of the barcoded cDNA is then performed in-chip. Full-length amplicons are pooled and then purified off-chip, and the sequencing libraries are prepared using a transposase-based method that enriches for cDNA derived from mRNA 3' ends followed by a limited-cycle PCR amplification step that results in incorporation of Illumina index sequences. Most importantly, the kit allows for efficient, cost-effective, and high-throughput single-cell gene expression analysis.

The ICELL8 Chip and Reagent 3' DE Kit enables Illumina sequencing and differential gene expression analysis—via transcript 3' end counting—of single cells isolated on the ICELL8 Single-Cell System. Following staining, dilution, and dispensation of cells into the 5,184-nanowell ICELL8 Chip v2, CellSelect software is used to automatically identify wells containing single, viable cells, and to direct nanowell-specific delivery of coupled RT-PCR reagents provided in the kit-including a 5' SMART primer. cDNA synthesis is oligo(dT)-primed using uniquely barcoded primers that have been preprinted into each nanowell of the ICELL8 Chip v2, and PCR amplification of the barcoded cDNA is then performed in-chip. Full-length amplicons are pooled and then purified off-chip, and the sequencing libraries are prepared using a transposase-based method that enriches for cDNA derived from mRNA 3' ends followed by a limited-cycle PCR amplification step that results in incorporation of Illumina index sequences. Most importantly, the kit allows for efficient, cost-effective, and high-throughput single-cell gene expression analysis.

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640166: ICELL8 Chip and Reagent 3' DE Kit

The ICELL8 3' DE Reagent Kit is part of the ICELL8 Chip and Reagent 3' DE Kit, which enables Illumina sequencing and differential gene expression analysis—via transcript 3' end counting—of single cells isolated on the ICELL8 cx Single-Cell System or ICELL8 Single-Cell System. Following staining, dilution, and dispensation of cells into a 5,184-nanowell ICELL8 3' DE Chip, CellSelect Software is used to automatically identify wells containing single, viable cells, and to direct nanowell-specific delivery of coupled RT-PCR reagents provided in the kit-including a 5' SMART primer provided in the ICELL8 3' DE Oligo Mix. cDNA synthesis is oligo(dT)-primed using uniquely barcoded primers that have been preprinted into each nanowell of the ICELL8 3' DE Chip, and PCR amplification of the barcoded cDNA is then performed in-chip. Full-length amplicons are pooled and purified off-chip, and sequencing libraries are prepared using a transposase-based method that enriches for cDNA derived from mRNA 3' ends followed by a limited-cycle PCR amplification step that results in incorporation of Illumina index sequences. Most importantly, the kit allows for efficient, cost-effective, and high-throughput single-cell gene expression analysis.

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640167: ICELL8 3' DE Reagent Kit

The ICELL8 Collection Kit consists of a fixture, tube, and film that allow users to spin down the contents of an ICELL8 chip to collect the contents of processed chips (e.g., extracted cDNA) into a tube.

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640048: ICELL8 Collection Kit

Required Products

Cat. #	Product	Size	Price	License	Quantity	Details
640000	ICELL8® Single-Cell System	Each	Inquire for Quotation	License Statement ID Number   329 This product is protected by one or more of these U.S. Patents 9,132,427, 9,909,171, 9,447,925, 9,951,381 and/or corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.	*
The ICELL8 Single-Cell System delivers unparalleled confidence and control for single-cell analysis by enabling precise cell isolation, cell selection, and high sample throughput. The system includes an ICELL8 MultiSample NanoDispenser, a complete imaging system—including microscope, computer, and software—a thermal cycler that accomodates ICELL8 chips, and additional accessories. Documents Components Image Data Back Isolate nuclei and cells of any type The ICELL8 MSND's large-bore nozzle allows unbiased isolation of any sample. A few of the cell types that have been isolated using the system are listed. For samples highlighted in blue, the ICELL8 Single-Cell System is the first to allow successful isolation of single cells, nuclei, or spheroids. Back Bioanalyzer traces from example TCR libraries Electropherogram profiles of TCR sequencing libraries. Electropherograms from three different cell pools (see technical note for more details): Pool 9 (Panel A), 10 (Panel B), and 11 (Panel C). The variation in the profiles reflects the abundance of amplified TCRa (peak at ~900 bp) and TCRb (peak at ~700 bp). Panel D. Positive Control RNA TCR library (generated with 8 x 5 pg Control Jurkat Total RNA). Panel E. Negative Control TCR library shows no library is produced. Back ICELL8 system overview Cell dispensing and visualization. After the ICELL8 MutliSample NanoDispenser has dispensed cells into the 5,184-nanowell chip, every well is imaged. Captured images are then processed in the CellSelect software to determine whether wells are empty, contain one cell, or contain multiple cells. This data can then be used to dispense reagents into the single-cell-containing wells. Back 640000: ICELL8 Single-Cell System

The ICELL8 3' DE for UMI Reagent Kit is used with the ICELL8 3' DE Chip, the ICELL8 Collection Kit, and the ICELL8 Loading Kit as part of the ICELL8 3' DE with UMI workflow, which enables Illumina sequencing and differential gene expression analysis—via transcript 3' end counting—of single cells isolated on the ICELL8 Single-Cell System.

The ICELL8 3' DE Chip is a component of the ICELL8 Single-Cell System, and is provided with application-specific SMARTer NGS kits designed for single-cell isolation and analysis. The ICELL8 3' DE Chip includes 5,184 nanowells, each of which contain a preprinted oligo(dT) primer with a nanowell-specific barcode. Samples and reagents are distributed into ICELL8 3' DE Chip nanowells by the ICELL8 MultiSample NanoDispenser (MSND). Included with the ICELL8 system is a thermal cycler that accommodates the ICELL8 3' DE Chip and can be used to perform in-chip sample processing reactions such as cDNA synthesis and PCR amplification. Products from in-chip reactions are then pooled together for further processing and analysis. Sequencing data generated from samples processed with the ICELL8 3' DE Chip are ultimately traced back to individual nanowells of the ICELL8 3' DE Chip using the nanowell-specific barcodes included in the preprinted oligo(dT) primers.

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640143: ICELL8 3' DE Chip

The ICELL8 Loading Kit contains films and blotting paper designed to be used with ICELL8 chips. The Blotting Paper is used to blot chips after dispensing of cells into a chip by the ICELL8 MultiSample NanoDispenser (MSND). The Optical Imaging Film is used to seal chips prior to imaging of the nanonwells with the ICELL8 Imaging System. The TE Sealing Film is used to seal the chip after reagents have been dispensed into cells selected for processing for reverse transcription.

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640109: ICELL8 Loading Kit

20 Pack of MSND 384-Well Source Plate and Seals. Formerly 430-000025.

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640018: MSND 384-Well Source Plate and Seals (430-000025)

120 pack of MSND 384-well Source Plate and Seals. Formerly 430-000258.

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640037: MSND 384-well Source Plate and Seals (430-000258)