SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian
NOTE: For strand-specific RNA-seq libraries from RNA-depleted or poly(A)-enriched samples, we recommend the reconfigured versions of the kit below, SMART-Seq Total RNA Mid Input (Cat. # 635048, 635049 & 635050).
The SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian (Cat. # 634861) will be phased out soon. Contact your local sales representative for more information.
The SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian provides a complete solution for sensitive and streamlined total RNA analysis. The kit is designed to work with a wide range of input amounts (10–100 ng) of total RNA of any quality. A variety of human, rat, and mouse samples can be used, including total RNA from FFPE material, tissue samples, etc. The kit provides whole transcriptome analysis with accurate measurement of strand orientation, unbiased coverage, high transcript mapping, and gene counts.
NOTE: For strand-specific RNA-seq libraries from RNA-depleted or poly(A)-enriched samples, we recommend the reconfigured versions of the kit below, SMART-Seq Total RNA Mid Input (Cat. # 635048, 635049 & 635050).
The SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian (Cat. # 634861) will be phased out soon. Contact your local sales representative for more information.
The SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian provides a complete solution for sensitive and streamlined total RNA analysis. The kit is designed to work with a wide range of input amounts (10–100 ng) of total RNA of any quality. A variety of human, rat, and mouse samples can be used, including total RNA from FFPE material, tissue samples, etc. The kit provides whole transcriptome analysis with accurate measurement of strand orientation, unbiased coverage, high transcript mapping, and gene counts.
This low-input kit contains the Illumina Indexing Primer Set, and the entire protocol—from rRNA depletion through the creation of Illumina-specific, indexed RNA-seq libraries—takes only about 5 hours.
Alternate kits for other input amounts:
- For 250 pg–10 ng of total RNA, we recommend the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian
- For 100 ng–1 µg of total RNA, we recommend the SMART-Seq Total RNA High Input (RiboGone Mammalian)
Overview
- Strand information—Identify each transcript’s strand of origin with >99% accuracy.
- Fast, streamlined protocol—Go from start to finish in ~5 hours.
- Variable input size—Use 10–100 ng of total RNA from human, mouse, or rat.
- Versatility—Get reliable data across replicates. See high-quality, reproducible data from total RNA with a RIN (RNA Integrity Number) from 3–10.
- Seamless integration with Illumina sequencing—Generate 24 uniquely indexed Illumina-ready libraries.
Interested in more data and FAQs about this product? Visit the NGS Learning Center.
More Information
Applications
- Robust NGS library construction that retains strand information
- Use for RNA-seq on all Illumina platforms
- Captures coding and noncoding information from total mammalian RNA of any quality, including RNA obtained from FFPE samples
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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