Legacy RNA-seq kits

The SMARTer Ultra Low RNA Kit allows high-quality cDNA synthesis starting from as little as 10 pg of total RNA or a single cell. The kit has been designed and validated to prepare cDNA samples for sequencing and quantitation with the Illumina HiSeq and Genome Analyzer sequencing instruments. The entire library construction protocol can be completed within two days. SMART technology offers unparalleled sensitivity and unbiased amplification of cDNA transcripts, enabling a direct start from your sample. Most importantly, SMART technology enriches for full-length transcripts and maintains the true representation of the original mRNA transcripts; these factors are critical for transcriptome sequencing and gene expression analysis.

Back

Comparison of transcript coverage with different amounts of input RNA

Comparison of transcript coverage with different amounts of input RNA. Shown are overlaid plots comparing the average read coverage from libraries made with 1 ng to 0.01 ng of mouse brain total RNA. The x-axis represents gene length normalized to 100%, where 0 is the 5’-end of each transcript and 100 is the 3’-end. The y-axis indicates the average coverage for a set of 724 genes that are moderately to highly expressed in brain tissue. The results are very consistent through the range of input RNA used, with full-length coverage of the transcripts reflecting no systematic 5’- or 3’-bias.

Back

Electropherogram of amplified SMARTer cDNA

Electropherogram of amplified SMARTer cDNA. Various amounts of Universal Human Reference Total RNA (UHR) and Human Brain Reference RNA were used as input for SMARTer cDNA synthesis.The cDNA samples were then analyzed for purity and yield on an Agilent 2100 Bioanalyzer. Shown are Bioanalyzer trace overlays of cDNA amplified from 1 ng (red line), 0.1 ng (dark blue line), 0.05 ng (green line), and 0.01 ng (light blue line) of total RNA and a no template control (NTC; pink line). The main peak indicates the purity and yield of cDNA between 0.4 and 9 kb—with the highest point at ~2 kb. There was no amplification in the negative control (pink line). Although the amount of input RNA can vary over quite a large range (e.g., 1 ng to 0.01 ng), comparable cDNA output can be obtained by adjusting the number of PCR cycles.

Back

Gene expression data obtained from very low amounts of RNA correlate well with data obtained by qPCR

Gene expression data obtained from very low amounts of RNA correlate well with data obtained by qPCR. Scatter plots were used to compare differential expression data obtained by sequencing with the SMARTer Ultra Low RNA Kit (1 ng total RNA) and quantitative PCR (qPCR) data available for Universal Human Reference Total RNA (UHR) and Human Brain Reference RNA through the MicroArray Quality Control (MAQC) project. The differential expression of ~700 genes showed correlation values of 0.94, demonstrating that the sequencing results are consistent with orthogonal gene expression technologies.

Back

Overview of SMARTer Ultra Low RNA Kit sample preparation for Illumina sequencing

Overview of SMARTer Ultra Low RNA Kit sample preparation for Illumina sequencing.

Back

634936: SMARTer Ultra Low RNA Kit for Illumina Sequencing

Required Products

Cat. #	Product	Size	Price	License	Quantity	Details
639207	Advantage® 2 PCR Kit	30 Rxns	USD $173.00
This kit is based on Takara's Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 30 PCR reactions of 50 μl each. Documents Components Image Data Back Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix. 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker Back Amplification of various large templates using Advantage 2 Polymerase Mix Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker. Back 639207: Advantage 2 PCR Kit
638504	SeqAmp™ DNA Polymerase	50 Rxns	USD $140.00
SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Back 638504: SeqAmp DNA Polymerase
638509	SeqAmp™ DNA Polymerase	200 Rxns	USD $389.00
SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back 638509: SeqAmp DNA Polymerase

The SMARTer Ultra Low Input RNA Kit for Sequencing - v3 allows direct synthesis of high-quality cDNA when starting with 1–1,000 cells or 10 pg–10 ng of total RNA. The kit has been designed and validated to prepare cDNA samples for library preparation and sequencing using the Ion Torrent or Illumina sequencing platforms. SMART (Switching Mechanism At 5' End of RNA Template) technology enriches for full-length transcripts and maintains the true representation of the original mRNA transcripts; these factors are critical for transcriptome sequencing and gene expression analysis. This kit improves on previous generations of SMARTer Ultra Low kits by simplifying the workflow, identifying more genes, and increasing the representation of GC-rich genes. It is a complete kit that supplies all required reagents, including SeqAmp DNA polymerase, an optimized PCR enzyme that has been verified to perform well with this kit.

Back

Comparison of sequencing metrics generated with either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

Comparison of sequencing metrics generated with either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3). cDNA libraries were generated from either 100 pg Human Brain Total RNA (HBR) or 10 pg Mouse Brain Total RNA (MBR) and were sequenced on an Illumina MiSeq platform. The increased sensitivity gained using the UL-v3 protocol is most significant at the lowest RNA inputs.

Back

Sequencing results for libraries made from 100 pg Human Brain Total RNA using either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

Sequencing results for libraries made from 100 pg Human Brain Total RNA using either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3). Genes were binned by GC content and correlation plots were used to evaluate the two kits. The average gene counts were very reproducible for replicate samples using UL-HV (Panel A) or UL-v3 (Panel B). When the two protocols were compared, genes with a high GC content (red) showed higher expression with the UL-v3 protocol while genes with a median or low GC content (gray and blue, respectively) showed similar expression levels with both protocols (Panel C).

Back

Individual cells or 1,000 cells (HeLa or Jurkat; Panel A and Panel B, respectively) were used as input for the SMARTer Ultra Low Input RNA Kit for Sequencing - v3

Individual cells or 1,000 cells (HeLa or Jurkat; Panel A and Panel B, respectively) were used as input for the SMARTer Ultra Low Input RNA Kit for Sequencing - v3. The cDNA samples were analyzed on an Agilent 2100 Bioanalyzer. The single main peak indicates the purity and yield of the cDNA (the additional peak at ~1 kb in the HeLa cell samples is the contribution of a single highly expressed gene, FTH1).

Back

cDNA libraries were made from 1 or 1,000 cells (HeLa or Jurkat) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

cDNA libraries were made from 1 or 1,000 cells (HeLa or Jurkat) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3). Table IIA. Results of Illumina library preparation and sequencing. Table IIB. Results of Ion Torrent library preparation and sequencing.

Back

The gene body coverage of the cDNA libraries made from 1 or 1,000 HeLa cells (blue and red lines, respectively), or 1 or 1,000 Jurkat cells (green and purple lines, respectively) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 was determined using RSeQC

The gene body coverage of the cDNA libraries made from 1 or 1,000 HeLa cells (blue and red lines, respectively), or 1 or 1,000 Jurkat cells (green and purple lines, respectively) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 was determined using RSeQC. Read coverage was normalized using Excel.

Required Products

Cat. #	Product	Size	Price	License	Quantity	Details
639207	Advantage® 2 PCR Kit	30 Rxns	USD $173.00
This kit is based on Takara's Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 30 PCR reactions of 50 μl each. Documents Components Image Data Back Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix. 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker Back Amplification of various large templates using Advantage 2 Polymerase Mix Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker. Back 639207: Advantage 2 PCR Kit
638504	SeqAmp™ DNA Polymerase	50 Rxns	USD $140.00
SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Back 638504: SeqAmp DNA Polymerase
638509	SeqAmp™ DNA Polymerase	200 Rxns	USD $389.00
SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back 638509: SeqAmp DNA Polymerase

The SMARTer Ultra Low Input RNA Kit for Sequencing - v3 allows direct synthesis of high-quality cDNA when starting with 1–1,000 cells or 10 pg–10 ng of total RNA. The kit has been designed and validated to prepare cDNA samples for library preparation and sequencing using the Ion Torrent or Illumina sequencing platforms. SMART (Switching Mechanism At 5' End of RNA Template) technology enriches for full-length transcripts and maintains the true representation of the original mRNA transcripts; these factors are critical for transcriptome sequencing and gene expression analysis. This kit improves on previous generations of SMARTer Ultra Low kits by simplifying the workflow, identifying more genes, and increasing the representation of GC-rich genes. It is a complete kit that supplies all required reagents, including SeqAmp DNA polymerase, an optimized PCR enzyme that has been verified to perform well with this kit.

Back

Comparison of sequencing metrics generated with either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

Comparison of sequencing metrics generated with either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3). cDNA libraries were generated from either 100 pg Human Brain Total RNA (HBR) or 10 pg Mouse Brain Total RNA (MBR) and were sequenced on an Illumina MiSeq platform. The increased sensitivity gained using the UL-v3 protocol is most significant at the lowest RNA inputs.

Back

Sequencing results for libraries made from 100 pg Human Brain Total RNA using either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

Sequencing results for libraries made from 100 pg Human Brain Total RNA using either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3). Genes were binned by GC content and correlation plots were used to evaluate the two kits. The average gene counts were very reproducible for replicate samples using UL-HV (Panel A) or UL-v3 (Panel B). When the two protocols were compared, genes with a high GC content (red) showed higher expression with the UL-v3 protocol while genes with a median or low GC content (gray and blue, respectively) showed similar expression levels with both protocols (Panel C).

Back

Individual cells or 1,000 cells (HeLa or Jurkat; Panel A and Panel B, respectively) were used as input for the SMARTer Ultra Low Input RNA Kit for Sequencing - v3

Individual cells or 1,000 cells (HeLa or Jurkat; Panel A and Panel B, respectively) were used as input for the SMARTer Ultra Low Input RNA Kit for Sequencing - v3. The cDNA samples were analyzed on an Agilent 2100 Bioanalyzer. The single main peak indicates the purity and yield of the cDNA (the additional peak at ~1 kb in the HeLa cell samples is the contribution of a single highly expressed gene, FTH1).

Back

cDNA libraries were made from 1 or 1,000 cells (HeLa or Jurkat) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

cDNA libraries were made from 1 or 1,000 cells (HeLa or Jurkat) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3). Table IIA. Results of Illumina library preparation and sequencing. Table IIB. Results of Ion Torrent library preparation and sequencing.

Back

The gene body coverage of the cDNA libraries made from 1 or 1,000 HeLa cells (blue and red lines, respectively), or 1 or 1,000 Jurkat cells (green and purple lines, respectively) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 was determined using RSeQC

The gene body coverage of the cDNA libraries made from 1 or 1,000 HeLa cells (blue and red lines, respectively), or 1 or 1,000 Jurkat cells (green and purple lines, respectively) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 was determined using RSeQC. Read coverage was normalized using Excel.

Required Products

Cat. #	Product	Size	Price	License	Quantity	Details
639207	Advantage® 2 PCR Kit	30 Rxns	USD $173.00
This kit is based on Takara's Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 30 PCR reactions of 50 μl each. Documents Components Image Data Back Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix. 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker Back Amplification of various large templates using Advantage 2 Polymerase Mix Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker. Back 639207: Advantage 2 PCR Kit
638504	SeqAmp™ DNA Polymerase	50 Rxns	USD $140.00
SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Back 638504: SeqAmp DNA Polymerase
638509	SeqAmp™ DNA Polymerase	200 Rxns	USD $389.00
SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back 638509: SeqAmp DNA Polymerase

The SMARTer Ultra Low Input RNA Kit for Sequencing - v3 allows direct synthesis of high-quality cDNA when starting with 1–1,000 cells or 10 pg–10 ng of total RNA. The kit has been designed and validated to prepare cDNA samples for library preparation and sequencing using the Ion Torrent or Illumina sequencing platforms. SMART (Switching Mechanism At 5' End of RNA Template) technology enriches for full-length transcripts and maintains the true representation of the original mRNA transcripts; these factors are critical for transcriptome sequencing and gene expression analysis. This kit improves on previous generations of SMARTer Ultra Low kits by simplifying the workflow, identifying more genes, and increasing the representation of GC-rich genes. It is a complete kit that supplies all required reagents, including SeqAmp DNA polymerase, an optimized PCR enzyme that has been verified to perform well with this kit.

Back

Comparison of sequencing metrics generated with either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

Comparison of sequencing metrics generated with either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3). cDNA libraries were generated from either 100 pg Human Brain Total RNA (HBR) or 10 pg Mouse Brain Total RNA (MBR) and were sequenced on an Illumina MiSeq platform. The increased sensitivity gained using the UL-v3 protocol is most significant at the lowest RNA inputs.

Back

Sequencing results for libraries made from 100 pg Human Brain Total RNA using either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

Sequencing results for libraries made from 100 pg Human Brain Total RNA using either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3). Genes were binned by GC content and correlation plots were used to evaluate the two kits. The average gene counts were very reproducible for replicate samples using UL-HV (Panel A) or UL-v3 (Panel B). When the two protocols were compared, genes with a high GC content (red) showed higher expression with the UL-v3 protocol while genes with a median or low GC content (gray and blue, respectively) showed similar expression levels with both protocols (Panel C).

Back

Individual cells or 1,000 cells (HeLa or Jurkat; Panel A and Panel B, respectively) were used as input for the SMARTer Ultra Low Input RNA Kit for Sequencing - v3

Individual cells or 1,000 cells (HeLa or Jurkat; Panel A and Panel B, respectively) were used as input for the SMARTer Ultra Low Input RNA Kit for Sequencing - v3. The cDNA samples were analyzed on an Agilent 2100 Bioanalyzer. The single main peak indicates the purity and yield of the cDNA (the additional peak at ~1 kb in the HeLa cell samples is the contribution of a single highly expressed gene, FTH1).

Back

cDNA libraries were made from 1 or 1,000 cells (HeLa or Jurkat) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

cDNA libraries were made from 1 or 1,000 cells (HeLa or Jurkat) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3). Table IIA. Results of Illumina library preparation and sequencing. Table IIB. Results of Ion Torrent library preparation and sequencing.

Back

The gene body coverage of the cDNA libraries made from 1 or 1,000 HeLa cells (blue and red lines, respectively), or 1 or 1,000 Jurkat cells (green and purple lines, respectively) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 was determined using RSeQC

The gene body coverage of the cDNA libraries made from 1 or 1,000 HeLa cells (blue and red lines, respectively), or 1 or 1,000 Jurkat cells (green and purple lines, respectively) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 was determined using RSeQC. Read coverage was normalized using Excel.

Required Products

Cat. #	Product	Size	Price	License	Quantity	Details
639207	Advantage® 2 PCR Kit	30 Rxns	USD $173.00
This kit is based on Takara's Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 30 PCR reactions of 50 μl each. Documents Components Image Data Back Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix. 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker Back Amplification of various large templates using Advantage 2 Polymerase Mix Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker. Back 639207: Advantage 2 PCR Kit
638504	SeqAmp™ DNA Polymerase	50 Rxns	USD $140.00
SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Back 638504: SeqAmp DNA Polymerase
638509	SeqAmp™ DNA Polymerase	200 Rxns	USD $389.00
SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back 638509: SeqAmp DNA Polymerase

The SMARTer Ultra Low Input RNA Kit for Sequencing - v3 allows direct synthesis of high-quality cDNA when starting with 1–1,000 cells or 10 pg–10 ng of total RNA. The kit has been designed and validated to prepare cDNA samples for library preparation and sequencing using the Ion Torrent or Illumina sequencing platforms. SMART (Switching Mechanism At 5' End of RNA Template) technology enriches for full-length transcripts and maintains the true representation of the original mRNA transcripts; these factors are critical for transcriptome sequencing and gene expression analysis. This kit improves on previous generations of SMARTer Ultra Low kits by simplifying the workflow, identifying more genes, and increasing the representation of GC-rich genes. It is a complete kit that supplies all required reagents, including SeqAmp DNA polymerase, an optimized PCR enzyme that has been verified to perform well with this kit.

Back

Comparison of sequencing metrics generated with either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

Comparison of sequencing metrics generated with either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3). cDNA libraries were generated from either 100 pg Human Brain Total RNA (HBR) or 10 pg Mouse Brain Total RNA (MBR) and were sequenced on an Illumina MiSeq platform. The increased sensitivity gained using the UL-v3 protocol is most significant at the lowest RNA inputs.

Back

Sequencing results for libraries made from 100 pg Human Brain Total RNA using either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

Sequencing results for libraries made from 100 pg Human Brain Total RNA using either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3). Genes were binned by GC content and correlation plots were used to evaluate the two kits. The average gene counts were very reproducible for replicate samples using UL-HV (Panel A) or UL-v3 (Panel B). When the two protocols were compared, genes with a high GC content (red) showed higher expression with the UL-v3 protocol while genes with a median or low GC content (gray and blue, respectively) showed similar expression levels with both protocols (Panel C).

Back

Individual cells or 1,000 cells (HeLa or Jurkat; Panel A and Panel B, respectively) were used as input for the SMARTer Ultra Low Input RNA Kit for Sequencing - v3

Individual cells or 1,000 cells (HeLa or Jurkat; Panel A and Panel B, respectively) were used as input for the SMARTer Ultra Low Input RNA Kit for Sequencing - v3. The cDNA samples were analyzed on an Agilent 2100 Bioanalyzer. The single main peak indicates the purity and yield of the cDNA (the additional peak at ~1 kb in the HeLa cell samples is the contribution of a single highly expressed gene, FTH1).

Back

cDNA libraries were made from 1 or 1,000 cells (HeLa or Jurkat) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

cDNA libraries were made from 1 or 1,000 cells (HeLa or Jurkat) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3). Table IIA. Results of Illumina library preparation and sequencing. Table IIB. Results of Ion Torrent library preparation and sequencing.

Back

The gene body coverage of the cDNA libraries made from 1 or 1,000 HeLa cells (blue and red lines, respectively), or 1 or 1,000 Jurkat cells (green and purple lines, respectively) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 was determined using RSeQC

The gene body coverage of the cDNA libraries made from 1 or 1,000 HeLa cells (blue and red lines, respectively), or 1 or 1,000 Jurkat cells (green and purple lines, respectively) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 was determined using RSeQC. Read coverage was normalized using Excel.

Required Products

Cat. #	Product	Size	Price	License	Quantity	Details
639207	Advantage® 2 PCR Kit	30 Rxns	USD $173.00
This kit is based on Takara's Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 30 PCR reactions of 50 μl each. Documents Components Image Data Back Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix. 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker Back Amplification of various large templates using Advantage 2 Polymerase Mix Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker. Back 639207: Advantage 2 PCR Kit
638504	SeqAmp™ DNA Polymerase	50 Rxns	USD $140.00
SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Back 638504: SeqAmp DNA Polymerase
638509	SeqAmp™ DNA Polymerase	200 Rxns	USD $389.00
SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back 638509: SeqAmp DNA Polymerase

The SMARTer Ultra Low Input RNA Kit for Sequencing - v3 allows direct synthesis of high-quality cDNA when starting with 1–1,000 cells or 10 pg–10 ng of total RNA. The kit has been designed and validated to prepare cDNA samples for library preparation and sequencing using the Ion Torrent or Illumina sequencing platforms. SMART (Switching Mechanism At 5' End of RNA Template) technology enriches for full-length transcripts and maintains the true representation of the original mRNA transcripts; these factors are critical for transcriptome sequencing and gene expression analysis. This kit improves on previous generations of SMARTer Ultra Low kits by simplifying the workflow, identifying more genes, and increasing the representation of GC-rich genes. It is a complete kit that supplies all required reagents, including SeqAmp DNA polymerase, an optimized PCR enzyme that has been verified to perform well with this kit.

Back

Comparison of sequencing metrics generated with either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

Comparison of sequencing metrics generated with either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3). cDNA libraries were generated from either 100 pg Human Brain Total RNA (HBR) or 10 pg Mouse Brain Total RNA (MBR) and were sequenced on an Illumina MiSeq platform. The increased sensitivity gained using the UL-v3 protocol is most significant at the lowest RNA inputs.

Back

Sequencing results for libraries made from 100 pg Human Brain Total RNA using either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

Sequencing results for libraries made from 100 pg Human Brain Total RNA using either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3). Genes were binned by GC content and correlation plots were used to evaluate the two kits. The average gene counts were very reproducible for replicate samples using UL-HV (Panel A) or UL-v3 (Panel B). When the two protocols were compared, genes with a high GC content (red) showed higher expression with the UL-v3 protocol while genes with a median or low GC content (gray and blue, respectively) showed similar expression levels with both protocols (Panel C).

Back

Individual cells or 1,000 cells (HeLa or Jurkat; Panel A and Panel B, respectively) were used as input for the SMARTer Ultra Low Input RNA Kit for Sequencing - v3

Individual cells or 1,000 cells (HeLa or Jurkat; Panel A and Panel B, respectively) were used as input for the SMARTer Ultra Low Input RNA Kit for Sequencing - v3. The cDNA samples were analyzed on an Agilent 2100 Bioanalyzer. The single main peak indicates the purity and yield of the cDNA (the additional peak at ~1 kb in the HeLa cell samples is the contribution of a single highly expressed gene, FTH1).

Back

cDNA libraries were made from 1 or 1,000 cells (HeLa or Jurkat) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

cDNA libraries were made from 1 or 1,000 cells (HeLa or Jurkat) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3). Table IIA. Results of Illumina library preparation and sequencing. Table IIB. Results of Ion Torrent library preparation and sequencing.

Back

The gene body coverage of the cDNA libraries made from 1 or 1,000 HeLa cells (blue and red lines, respectively), or 1 or 1,000 Jurkat cells (green and purple lines, respectively) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 was determined using RSeQC

The gene body coverage of the cDNA libraries made from 1 or 1,000 HeLa cells (blue and red lines, respectively), or 1 or 1,000 Jurkat cells (green and purple lines, respectively) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 was determined using RSeQC. Read coverage was normalized using Excel.

Required Products

Cat. #	Product	Size	Price	License	Quantity	Details
639207	Advantage® 2 PCR Kit	30 Rxns	USD $173.00
This kit is based on Takara's Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 30 PCR reactions of 50 μl each. Documents Components Image Data Back Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix. 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker Back Amplification of various large templates using Advantage 2 Polymerase Mix Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker. Back 639207: Advantage 2 PCR Kit
638504	SeqAmp™ DNA Polymerase	50 Rxns	USD $140.00
SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Back 638504: SeqAmp DNA Polymerase
638509	SeqAmp™ DNA Polymerase	200 Rxns	USD $389.00
SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back 638509: SeqAmp DNA Polymerase

SMARTer Ultra Low Input RNA for Illumina Sequencing - HV allows high-quality cDNA synthesis when starting with very low input amounts of total RNA. The kit is regularly tested with 100 pg of total RNA, and has been shown to perform robust cDNA synthesis with RNA from a single cell (10 pg equivalent). It includes the Advantage 2 PCR Kit for PCR amplification and validation. The kit has been designed and validated to prepare cDNA samples for sequencing and quantitation with the Illumina HiSeq, MiSeq, and Genome Analyzer sequencing instruments. SMART technology offers unparalleled sensitivity and unbiased amplification of cDNA transcripts, enabling a direct start from your sample. Most importantly, SMART technology enriches for full-length transcripts and maintains the true representation of the original mRNA transcripts; these factors are critical for transcriptome sequencing and gene expression analysis. This kit accommodates an input volume of up to 9 μl.

Back

Comparison of transcript coverage with different amounts of input RNA

Comparison of transcript coverage with different amounts of input RNA. Shown are overlaid plots comparing the average read coverage from libraries made with 1 ng to 0.01 ng of mouse brain total RNA. The x-axis represents gene length normalized to 100%, where 0 is the 5’-end of each transcript and 100 is the 3’-end. The y-axis indicates the average coverage for a set of 724 genes that are moderately to highly expressed in brain tissue. The results are very consistent through the range of input RNA used, with full-length coverage of the transcripts reflecting no systematic 5’- or 3’-bias.

Back

Electropherogram of amplified SMARTer cDNA

Electropherogram of amplified SMARTer cDNA. Various amounts of Universal Human Reference Total RNA (UHR) and Human Brain Reference RNA were used as input for SMARTer cDNA synthesis.The cDNA samples were then analyzed for purity and yield on an Agilent 2100 Bioanalyzer. Shown are Bioanalyzer trace overlays of cDNA amplified from 1 ng (red line), 0.1 ng (dark blue line), 0.05 ng (green line), and 0.01 ng (light blue line) of total RNA and a no template control (NTC; pink line). The main peak indicates the purity and yield of cDNA between 0.4 and 9 kb—with the highest point at ~2 kb. There was no amplification in the negative control (pink line). Although the amount of input RNA can vary over quite a large range (e.g., 1 ng to 0.01 ng), comparable cDNA output can be obtained by adjusting the number of PCR cycles.

Back

Gene expression data obtained from very low amounts of RNA correlate well with data obtained by qPCR

Gene expression data obtained from very low amounts of RNA correlate well with data obtained by qPCR. Scatter plots were used to compare differential expression data obtained by sequencing with the SMARTer Ultra Low RNA Kit (1 ng total RNA) and quantitative PCR (qPCR) data available for Universal Human Reference Total RNA (UHR) and Human Brain Reference RNA through the MicroArray Quality Control (MAQC) project. The differential expression of ~700 genes showed correlation values of 0.94, demonstrating that the sequencing results are consistent with orthogonal gene expression technologies.

Back

Overview of SMARTer Ultra Low RNA Kit sample preparation for Illumina sequencing

Overview of SMARTer Ultra Low RNA Kit sample preparation for Illumina sequencing.

Back

634828: SMARTer Ultra Low Input RNA for Illumina Sequencing - HV

Required Products

Cat. #	Product	Size	Price	License	Quantity	Details
639207	Advantage® 2 PCR Kit	30 Rxns	USD $173.00
This kit is based on Takara's Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 30 PCR reactions of 50 μl each. Documents Components Image Data Back Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix. 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker Back Amplification of various large templates using Advantage 2 Polymerase Mix Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker. Back 639207: Advantage 2 PCR Kit
638504	SeqAmp™ DNA Polymerase	50 Rxns	USD $140.00
SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Back 638504: SeqAmp DNA Polymerase
638509	SeqAmp™ DNA Polymerase	200 Rxns	USD $389.00
SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back 638509: SeqAmp DNA Polymerase

SMARTer Ultra Low Input RNA for Illumina Sequencing - HV allows high-quality cDNA synthesis when starting with very low input amounts of total RNA. The kit is regularly tested with 100 pg of total RNA, and has been shown to perform robust cDNA synthesis with RNA from a single cell (10 pg equivalent). It includes the Advantage 2 PCR Kit for PCR amplification and validation. The kit has been designed and validated to prepare cDNA samples for sequencing and quantitation with the Illumina HiSeq, MiSeq, and Genome Analyzer sequencing instruments. SMART technology offers unparalleled sensitivity and unbiased amplification of cDNA transcripts, enabling a direct start from your sample. Most importantly, SMART technology enriches for full-length transcripts and maintains the true representation of the original mRNA transcripts; these factors are critical for transcriptome sequencing and gene expression analysis. This kit accommodates an input volume of up to 9 μl.

Back

Comparison of transcript coverage with different amounts of input RNA

Comparison of transcript coverage with different amounts of input RNA. Shown are overlaid plots comparing the average read coverage from libraries made with 1 ng to 0.01 ng of mouse brain total RNA. The x-axis represents gene length normalized to 100%, where 0 is the 5’-end of each transcript and 100 is the 3’-end. The y-axis indicates the average coverage for a set of 724 genes that are moderately to highly expressed in brain tissue. The results are very consistent through the range of input RNA used, with full-length coverage of the transcripts reflecting no systematic 5’- or 3’-bias.

Back

Electropherogram of amplified SMARTer cDNA

Electropherogram of amplified SMARTer cDNA. Various amounts of Universal Human Reference Total RNA (UHR) and Human Brain Reference RNA were used as input for SMARTer cDNA synthesis.The cDNA samples were then analyzed for purity and yield on an Agilent 2100 Bioanalyzer. Shown are Bioanalyzer trace overlays of cDNA amplified from 1 ng (red line), 0.1 ng (dark blue line), 0.05 ng (green line), and 0.01 ng (light blue line) of total RNA and a no template control (NTC; pink line). The main peak indicates the purity and yield of cDNA between 0.4 and 9 kb—with the highest point at ~2 kb. There was no amplification in the negative control (pink line). Although the amount of input RNA can vary over quite a large range (e.g., 1 ng to 0.01 ng), comparable cDNA output can be obtained by adjusting the number of PCR cycles.

Back

Gene expression data obtained from very low amounts of RNA correlate well with data obtained by qPCR

Gene expression data obtained from very low amounts of RNA correlate well with data obtained by qPCR. Scatter plots were used to compare differential expression data obtained by sequencing with the SMARTer Ultra Low RNA Kit (1 ng total RNA) and quantitative PCR (qPCR) data available for Universal Human Reference Total RNA (UHR) and Human Brain Reference RNA through the MicroArray Quality Control (MAQC) project. The differential expression of ~700 genes showed correlation values of 0.94, demonstrating that the sequencing results are consistent with orthogonal gene expression technologies.

Back

Overview of SMARTer Ultra Low RNA Kit sample preparation for Illumina sequencing

Overview of SMARTer Ultra Low RNA Kit sample preparation for Illumina sequencing.

Required Products

Cat. #	Product	Size	Price	License	Quantity	Details
639207	Advantage® 2 PCR Kit	30 Rxns	USD $173.00
This kit is based on Takara's Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 30 PCR reactions of 50 μl each. Documents Components Image Data Back Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix. 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker Back Amplification of various large templates using Advantage 2 Polymerase Mix Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker. Back 639207: Advantage 2 PCR Kit
638504	SeqAmp™ DNA Polymerase	50 Rxns	USD $140.00
SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Back 638504: SeqAmp DNA Polymerase
638509	SeqAmp™ DNA Polymerase	200 Rxns	USD $389.00
SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back 638509: SeqAmp DNA Polymerase

SMARTer Ultra Low Input RNA for Illumina Sequencing - HV allows high-quality cDNA synthesis when starting with very low input amounts of total RNA. The kit is regularly tested with 100 pg of total RNA, and has been shown to perform robust cDNA synthesis with RNA from a single cell (10 pg equivalent). It includes the Advantage 2 PCR Kit for PCR amplification and validation. The kit has been designed and validated to prepare cDNA samples for sequencing and quantitation with the Illumina HiSeq, MiSeq, and Genome Analyzer sequencing instruments. SMART technology offers unparalleled sensitivity and unbiased amplification of cDNA transcripts, enabling a direct start from your sample. Most importantly, SMART technology enriches for full-length transcripts and maintains the true representation of the original mRNA transcripts; these factors are critical for transcriptome sequencing and gene expression analysis. This kit accommodates an input volume of up to 9 μl.

Back

Comparison of transcript coverage with different amounts of input RNA

Comparison of transcript coverage with different amounts of input RNA. Shown are overlaid plots comparing the average read coverage from libraries made with 1 ng to 0.01 ng of mouse brain total RNA. The x-axis represents gene length normalized to 100%, where 0 is the 5’-end of each transcript and 100 is the 3’-end. The y-axis indicates the average coverage for a set of 724 genes that are moderately to highly expressed in brain tissue. The results are very consistent through the range of input RNA used, with full-length coverage of the transcripts reflecting no systematic 5’- or 3’-bias.

Back

Electropherogram of amplified SMARTer cDNA

Electropherogram of amplified SMARTer cDNA. Various amounts of Universal Human Reference Total RNA (UHR) and Human Brain Reference RNA were used as input for SMARTer cDNA synthesis.The cDNA samples were then analyzed for purity and yield on an Agilent 2100 Bioanalyzer. Shown are Bioanalyzer trace overlays of cDNA amplified from 1 ng (red line), 0.1 ng (dark blue line), 0.05 ng (green line), and 0.01 ng (light blue line) of total RNA and a no template control (NTC; pink line). The main peak indicates the purity and yield of cDNA between 0.4 and 9 kb—with the highest point at ~2 kb. There was no amplification in the negative control (pink line). Although the amount of input RNA can vary over quite a large range (e.g., 1 ng to 0.01 ng), comparable cDNA output can be obtained by adjusting the number of PCR cycles.

Back

Gene expression data obtained from very low amounts of RNA correlate well with data obtained by qPCR

Gene expression data obtained from very low amounts of RNA correlate well with data obtained by qPCR. Scatter plots were used to compare differential expression data obtained by sequencing with the SMARTer Ultra Low RNA Kit (1 ng total RNA) and quantitative PCR (qPCR) data available for Universal Human Reference Total RNA (UHR) and Human Brain Reference RNA through the MicroArray Quality Control (MAQC) project. The differential expression of ~700 genes showed correlation values of 0.94, demonstrating that the sequencing results are consistent with orthogonal gene expression technologies.

Back

Overview of SMARTer Ultra Low RNA Kit sample preparation for Illumina sequencing

Overview of SMARTer Ultra Low RNA Kit sample preparation for Illumina sequencing.

Required Products

Cat. #	Product	Size	Price	License	Quantity	Details
639207	Advantage® 2 PCR Kit	30 Rxns	USD $173.00
This kit is based on Takara's Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 30 PCR reactions of 50 μl each. Documents Components Image Data Back Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix. 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker Back Amplification of various large templates using Advantage 2 Polymerase Mix Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker. Back 639207: Advantage 2 PCR Kit
638504	SeqAmp™ DNA Polymerase	50 Rxns	USD $140.00
SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Back 638504: SeqAmp DNA Polymerase
638509	SeqAmp™ DNA Polymerase	200 Rxns	USD $389.00
SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back 638509: SeqAmp DNA Polymerase

The SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian is used to generate strand-specific RNA-seq libraries for Illumina sequencing using purified total RNA input ranging from 250 pg–10 ng. This kit takes advantage of SMARTer Stranded RNA-seq technology, based on our proprietary SMART (Switching Mechanism at the 5' end of RNA Template) technology, with the added benefits of locked nucleic acid (LNA) technology as in the SMART-Seq v4 kit. The integrated post-cDNA synthesis removal of abundant molecules originating from rRNA makes the workflow extremely sensitive with excellent reproducibility and low mapping to rRNA. This method was developed to work with either high- or low-quality total RNA and does not require additional rRNA removal methods or kits. Final libraries retain strand of origin information.

Back

High reproducibility across the recommended input range

High reproducibility across the recommended input range. Comparison of FPKMs from libraries generated with 250 pg and 10 ng of mouse brain total RNA using the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian. FPKM values are shown on a Log₁₀ scale. Transcripts represented in only one library can be seen along the x- and y-axes of the scatter plot.

Back

Schematic of the technologies in the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian

Schematic of the technologies in the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian. This workflow allows users to generate Illumina-compatible libraries for RNA-seq experiments. After library preparation and purification, the total time from input RNA to RNA-seq library is approximately five hours.

Back

Improved exon mapping and transcript identification from human tissues

Improved exon mapping and transcript identification from human tissues. Distribution of reads in libraries prepared with the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian in the absence (–) or presence (+) of R-Probes, using 250 pg of human total RNA. Sequences corresponding to 1 million paired-end reads per library were analyzed.

Back

Improved exon mapping and transcript identification from rodent tissues

Improved exon mapping and transcript identification from rodent tissues. Distribution of reads in libraries prepared with the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian in the absence (–) or presence (+) of R-Probes, using 250 or 500 pg of rat or mouse total RNA. Sequences corresponding to 1 million paired-end reads per library were analyzed.

Back

High reproducibility between libraries with or without ribosomal cDNA depletion

High reproducibility between libraries with or without ribosomal cDNA depletion. Comparison of FPKMs from libraries generated with 250 pg human brain or heart total RNA with (+) or without (-) ribosomal cDNA depletion (i.e., with or without R-Probes, respectively) using the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian. FPKM values are shown on a Log₁₀ scale. Transcripts represented in only one library can be seen along the x- and y-axes of the scatter plots.

Back

Efficient capture of degraded RNA

Efficient capture of degraded RNA. Distribution of insert sizes are shown for libraries generated from 250 pg of high-quality (RIN 8) or highly degraded (RIN 2.5) human total RNA using the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian with a 4 min shearing time (RIN 8) or with the alternate no-shearing protocol (RIN 2.5). RNA-seq libraries were sequenced on an Illumina MiSeq instrument in paired-end mode. The number of mapped fragments with any given insert size was normalized to the total number of fragments in the library. Fragments mapping to rRNA or the mitochondrial genome were excluded

Back

635007: SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian

Required Products

Cat. #	Product	Size	Price	License	Quantity	Details
740815.50	MN Bead Tubes Type E	50 Preps	USD $125.00
MN Bead Tubes are 2-ml screw cap plastic tubes containing different types of beads depending on the application. They are intended for the disruption of biological samples and subsequent nucleic acid purification. MN Bead Tubes Type E consists of a mixture of 40–400 µm glass and 3-mm steel beads for use with hard-to-lyse bacteria within tissue or insect samples using the NucleoSpin Lipid Tissue or NucleoSpin DNA Insect kits. The beads should be used in combination with the MN Bead Tube Holder (Cat. # 740469) and an appropriate vortexer (e.g., Vortex-Genie 2 from Scientific Industries), or with a mixer mill (e.g., from Retsch GmbH). Documents Image Data Back 740815.50: NucleoSpin Bead Tubes Type E

The SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian is used to generate strand-specific RNA-seq libraries for Illumina sequencing using purified total RNA input ranging from 250 pg–10 ng. This kit takes advantage of SMARTer Stranded RNA-seq technology, based on our proprietary SMART (Switching Mechanism at the 5' end of RNA Template) technology, with the added benefits of locked nucleic acid (LNA) technology as in the SMART-Seq v4 kit. The integrated post-cDNA synthesis removal of abundant molecules originating from rRNA makes the workflow extremely sensitive with excellent reproducibility and low mapping to rRNA. This method was developed to work with either high- or low-quality total RNA and does not require additional rRNA removal methods or kits. Final libraries retain strand of origin information.

Back

High reproducibility across the recommended input range

High reproducibility across the recommended input range. Comparison of FPKMs from libraries generated with 250 pg and 10 ng of mouse brain total RNA using the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian. FPKM values are shown on a Log₁₀ scale. Transcripts represented in only one library can be seen along the x- and y-axes of the scatter plot.

Back

Schematic of the technologies in the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian

Schematic of the technologies in the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian. This workflow allows users to generate Illumina-compatible libraries for RNA-seq experiments. After library preparation and purification, the total time from input RNA to RNA-seq library is approximately five hours.

Back

Improved exon mapping and transcript identification from human tissues

Improved exon mapping and transcript identification from human tissues. Distribution of reads in libraries prepared with the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian in the absence (–) or presence (+) of R-Probes, using 250 pg of human total RNA. Sequences corresponding to 1 million paired-end reads per library were analyzed.

Back

Improved exon mapping and transcript identification from rodent tissues

Improved exon mapping and transcript identification from rodent tissues. Distribution of reads in libraries prepared with the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian in the absence (–) or presence (+) of R-Probes, using 250 or 500 pg of rat or mouse total RNA. Sequences corresponding to 1 million paired-end reads per library were analyzed.

Back

High reproducibility between libraries with or without ribosomal cDNA depletion

High reproducibility between libraries with or without ribosomal cDNA depletion. Comparison of FPKMs from libraries generated with 250 pg human brain or heart total RNA with (+) or without (-) ribosomal cDNA depletion (i.e., with or without R-Probes, respectively) using the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian. FPKM values are shown on a Log₁₀ scale. Transcripts represented in only one library can be seen along the x- and y-axes of the scatter plots.

Back

Efficient capture of degraded RNA

Efficient capture of degraded RNA. Distribution of insert sizes are shown for libraries generated from 250 pg of high-quality (RIN 8) or highly degraded (RIN 2.5) human total RNA using the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian with a 4 min shearing time (RIN 8) or with the alternate no-shearing protocol (RIN 2.5). RNA-seq libraries were sequenced on an Illumina MiSeq instrument in paired-end mode. The number of mapped fragments with any given insert size was normalized to the total number of fragments in the library. Fragments mapping to rRNA or the mitochondrial genome were excluded

Back

635006: SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian

The SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian is used to generate strand-specific RNA-seq libraries for Illumina sequencing using purified total RNA input ranging from 250 pg–10 ng. This kit takes advantage of SMARTer Stranded RNA-seq technology, based on our proprietary SMART (Switching Mechanism at the 5' end of RNA Template) technology, with the added benefits of locked nucleic acid (LNA) technology as in the SMART-Seq v4 kit. The integrated post-cDNA synthesis removal of abundant molecules originating from rRNA makes the workflow extremely sensitive with excellent reproducibility and low mapping to rRNA. This method was developed to work with either high- or low-quality total RNA and does not require additional rRNA removal methods or kits. Final libraries retain strand of origin information.

Back

High reproducibility across the recommended input range

High reproducibility across the recommended input range. Comparison of FPKMs from libraries generated with 250 pg and 10 ng of mouse brain total RNA using the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian. FPKM values are shown on a Log₁₀ scale. Transcripts represented in only one library can be seen along the x- and y-axes of the scatter plot.

Back

Schematic of the technologies in the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian

Schematic of the technologies in the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian. This workflow allows users to generate Illumina-compatible libraries for RNA-seq experiments. After library preparation and purification, the total time from input RNA to RNA-seq library is approximately five hours.

Back

Improved exon mapping and transcript identification from human tissues

Improved exon mapping and transcript identification from human tissues. Distribution of reads in libraries prepared with the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian in the absence (–) or presence (+) of R-Probes, using 250 pg of human total RNA. Sequences corresponding to 1 million paired-end reads per library were analyzed.

Back

Improved exon mapping and transcript identification from rodent tissues

Improved exon mapping and transcript identification from rodent tissues. Distribution of reads in libraries prepared with the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian in the absence (–) or presence (+) of R-Probes, using 250 or 500 pg of rat or mouse total RNA. Sequences corresponding to 1 million paired-end reads per library were analyzed.

Back

High reproducibility between libraries with or without ribosomal cDNA depletion

High reproducibility between libraries with or without ribosomal cDNA depletion. Comparison of FPKMs from libraries generated with 250 pg human brain or heart total RNA with (+) or without (-) ribosomal cDNA depletion (i.e., with or without R-Probes, respectively) using the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian. FPKM values are shown on a Log₁₀ scale. Transcripts represented in only one library can be seen along the x- and y-axes of the scatter plots.

Back

Efficient capture of degraded RNA

Efficient capture of degraded RNA. Distribution of insert sizes are shown for libraries generated from 250 pg of high-quality (RIN 8) or highly degraded (RIN 2.5) human total RNA using the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian with a 4 min shearing time (RIN 8) or with the alternate no-shearing protocol (RIN 2.5). RNA-seq libraries were sequenced on an Illumina MiSeq instrument in paired-end mode. The number of mapped fragments with any given insert size was normalized to the total number of fragments in the library. Fragments mapping to rRNA or the mitochondrial genome were excluded

The SMARTer Ultra Low RNA Kit for the Fluidigm C1 System allows high-quality cDNA synthesis starting from 96 single cells that have been isolated and processed with the Fluidigm C1 Single-Cell Auto Prep System. The kit utilizes SMART technology to enrich for full-length transcripts and maintain the true representation of the original mRNA transcripts (critical factors for transcriptome sequencing and gene expression analysis), and includes the Advantage 2 PCR Kit for PCR amplification and validation. The kit has been designed to prepare cDNA samples for transcriptome analysis on any Illumina Genome Analyzer, HiSeq, HiScan or MiSeq sequencing instrument.

Back

634833: SMARTer Ultra Low RNA Kit for the Fluidigm C1 System, 10 IFCs

The SMARTer Ultra Low RNA Kit for the Fluidigm C1 System allows high-quality cDNA synthesis starting from 96 single cells that have been isolated and processed with the Fluidigm C1 Single-Cell Auto Prep System. The kit utilizes SMART technology to enrich for full-length transcripts and maintain the true representation of the original mRNA transcripts (critical factors for transcriptome sequencing and gene expression analysis), and includes the Advantage 2 PCR Kit for PCR amplification and validation. The kit has been designed to prepare cDNA samples for transcriptome analysis on any Illumina Genome Analyzer, HiSeq, HiScan or MiSeq sequencing instrument.

Back

634832: SMARTer Ultra Low RNA Kit for the Fluidigm C1 System, 2 IFCs