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  • SMART-Seq HT for streamlined mRNA-seq
  • SMART-Seq v4 for mRNA-seq with the Fluidigm C1
Technical notes View data for this product
Home › Products › Next-generation sequencing › RNA-seq › Kits for automation › SMART-Seq v4 for mRNA-seq with the Fluidigm C1

RNA-seq

  • Single-cell RNA-seq
    • SMART-Seq Single Cell for scRNA-seq
    • SMART-Seq Stranded for total RNA-seq
  • Ultra-low input RNA-seq
    • SMART-Seq v4 for mRNA-seq
    • SMART-Seq v4 3’ DE for mRNA-seq end counting
    • SMART-Seq DE3 Demultiplexer
  • Stranded RNA-seq for mammalian samples
    • Pico-input strand-specific total RNA-seq for mammalian samples
    • Low-input strand-specific total RNA-seq for mammalian samples
    • High-input strand-specific total RNA-seq for mammalian samples
  • Kits for automation
    • SMART-Seq HT for streamlined mRNA-seq
    • SMART-Seq v4 for mRNA-seq with the Fluidigm C1
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Technical notes View data for this product

SMART-Seq v4 Ultra Low RNA Kit for the Fluidigm C1 System—high performance single-cell transcriptome analysis

Single-cell RNA-seq enables researchers to study how gene expression patterns vary within cell populations. Takara Bio has developed robust tools for performing RNA-seq on individual cells isolated in Fluidigm's integrated fluidic circuit (IFC) system. The sensitive SMARTer chemistry of our ultra-low input kits paired with the high-throughput automation capability of the Fluidigm C1 system offer high-resolution insights for transcriptome analysis.

We currently offer two generations of single-cell mRNA-seq kits for the Fluidigm C1 Single Cell Auto Prep system. The newest version, the SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System, incorporates features from our industry-leading SMART-Seq v4 kit, including SMART (Switching Mechanism at 5’ End of RNA Template) and locked nucleic acid (LNA) technologies, which are collectively referred to as "SMARTer-seq® chemistry". This kit outperforms published protocols and our previous version, the SMARTer Ultra Low RNA Kit for the Fluidigm C1 System, by providing higher sensitivity, better reproducibility, and an improved representation of GC- and AT-rich genes. The new script for using this kit is supported by the mRNA Seq IFC and the Open App IFC, and can be downloaded through the Fluidigm Script Hub.

Cat. # Product Size Price License Quantity Details
635026 SMART-Seq® v4 Ultra® Low Input RNA Kit for the Fluidigm® C1™ System, 10 IFCs Each USD $2654.00

License Statement

ID Number  
M54 This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts. 
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

The SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System, IFCs allows high-quality cDNA synthesis starting from 96 single cells that have been isolated and processed with the Fluidigm C1 Single-Cell Auto Prep System. Employing locked nucleic acid (LNA) technology integrated with SMART technology, this kit offers remarkable sensitivity, enriches for full-length transcripts, and maintains true representation of the original mRNA-critical factors for transcriptome analysis. Genes with high GC content show higher expression with this kit as compared to the previous generation. The kit includes SeqAmp DNA Polymerase for PCR amplification, and the libraries generated by this kit have been tested for compatibility with Illumina sequencing platforms.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System, 10 IFCs Product Photo (635026)

SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System, 10 IFCs Product Photo (635026)
635025 SMART-Seq® v4 Ultra® Low Input RNA Kit for the Fluidigm® C1™ System, 4 IFCs Each USD $1323.00

License Statement

ID Number  
M54 This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts. 
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

The SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System, IFCs allows high-quality cDNA synthesis starting from 96 single cells that have been isolated and processed with the Fluidigm C1 Single-Cell Auto Prep System. Employing locked nucleic acid (LNA) technology integrated with SMART technology, this kit offers remarkable sensitivity, enriches for full-length transcripts, and maintains true representation of the original mRNA-critical factors for transcriptome analysis. Genes with high GC content show higher expression with this kit as compared to the previous generation. The kit includes SeqAmp DNA Polymerase for PCR amplification, and the libraries generated by this kit have been tested for compatibility with Illumina sequencing platforms.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components

Overview

  • Improved SMART-technology-based chemistry—LNA technology in the SMART-Seq v4 Oligo and improved chemistry lead to better performance (higher sensitivity, greater reproducibility, and more GC-rich genes detected).
  • Library construction in 2 days—The cDNA synthesis protocol takes approximately two hours, followed by the eight-hour SMART-Seq v4 script on the C1 instrument. The entire library construction protocol can be completed within two working days.
  • New script—The accompanying script is supported for use on both the mRNA Seq IFC and the Open App IFC. The SMART-Seq v4 script is available at the Fluidigm Script Hub. The user manual for this product is available on the Documents tab of this web page.

Interested in learning more about this product? Visit the tech note.

More Information

Applications

  • cDNA synthesis for mRNA-seq from up to 96 single cells on the Fluidigm C1 System
  • cDNA synthesis for qPCR analysis on the Fluidigm Biomark platform prior to Illumina sequencing

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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