Epigenetics and small RNA sequencing
Epigenetic profiling involves characterizing chromatin structure, DNA methylation states, and protein-DNA interactions, while small noncoding RNAs (smRNAs) and microRNAs (miRNAs) have diverse cellular functions affecting gene expression. Our NGS portfolio includes products to make your analysis of epigenetics, protein-DNA interactions, and small RNAs easier.
Epigenetic profiling involves characterizing chromatin structure, DNA methylation states, and protein-DNA interactions, while small noncoding RNAs (smRNAs) and microRNAs (miRNAs) have diverse cellular functions affecting gene expression. Our NGS portfolio includes products to make your analysis of epigenetics, protein-DNA interactions, and small RNAs easier. Information about the epigenome can be used to predict the developmental potential of cells and to understand gene regulatory mechanisms during development and disease progression. ChIP-seq (chromatin immunoprecipitation and sequencing) has been used extensively to identify changes in the histone occupation of chromatin, but it can also be used to identify where transcription factors or other DNA binding proteins interact with the genome. Our ChIP-seq kits provide a fast and simple method to recover crosslinked DNA and create sequencing libraries from protein-DNA complexes. The EpiXplore Meth-Seq DNA Enrichment Kit makes preparing sequencing libraries from both methylated and unmethylated DNA fractions quick and easy. These ligation-free library preparation methods are enabled by DNA SMART technology, enabling the generation of reproducible libraries from low-input samples.
The cellular functions of smRNAs and miRNAs are quite varied, including regulating RNA splicing and protein translation, affecting DNA replication by altering chromatin structure, mediating intercessory communication in a hormone-like fashion, and participating in genome defense. However, it can be difficult to accurately quantify these important RNA species. Our smRNA-seq kit captures miRNAs, siRNAs, piRNAs, and snoRNAs and relies on our SMART (Switching Mechanism at 5' End of RNA Template) technology to avoid ligation biases, while our microRNA-seq kit is specifically designed to identify miRNA using proprietary MAGIC (Mono-Adapter liGation and Intramolecular Circularization) technology which minimizes ligation bias.
For additional information on the advantages of our kits, or to access experimental procedures and sample data, please refer to our technical notes for chromatin immunoprecipitation sequencing (ChIP-seq), methylated DNA sequencing (meth-seq), small RNA sequencing (smRNA-seq), and microRNA sequencing (microRNA-seq).
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