ThruPLEX Plasma-Seq Kit
ThruPLEX Plasma-Seq Kits are powered by SMARTer ThruPLEX chemistry to generate high-performance NGS libraries from cell-free DNA isolated from plasma.
The chemistry has been specifically optimized for cell-free DNA to maximize library complexity and to preserve the GC representation of the input sample. Libraries can be used for copy number variation (CNV) analysis, whole genome-sequencing applications, or enrichment using your custom panels or leading target enrichment platforms, such as Agilent SureSelect and Roche NimbleGen SeqCap EZ.
ThruPLEX Plasma-Seq Kits are powered by SMARTer ThruPLEX chemistry to generate high-performance NGS libraries from cell-free DNA isolated from plasma.
The chemistry has been specifically optimized for cell-free DNA to maximize library complexity and to preserve the GC representation of the input sample. Libraries can be used for copy number variation (CNV) analysis, whole genome-sequencing applications, or enrichment using your custom panels or leading target enrichment platforms, such as Agilent SureSelect and Roche NimbleGen SeqCap EZ.
Libraries prepared with ThruPLEX Plasma-Seq Kits provide sensitivity to better understand genetic variations found within the plasma samples. Plasma, along with other liquid biopsies, has gained significant interest in the research community as a valuable and easily available sample type. These communities include translational research in oncology and developmental research involving the fetal fraction derived from maternal plasma.
Overview
- Cell-free DNA compatible—newly formulated repair and ligation reagents with optimized protocols
- High-performance NGS libraries—high library diversity with broad and reproducible GC coverage
- Variable sample input—<1 ng to 30 ng of cell-free DNA
- Fast and simple workflow—three steps in a single tube or well in two hours with no purification or sample transfer steps (tubes and plates sold separately)
- Wide range of research applications—construction of libraries from samples where results require high sensitivity, including liquid biopsies, circulating tumor DNA (ctDNA), and targeted sequencing
- Automation friendly—simple three-step dispensing workflow makes it easy to automate
More Information
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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