T7 RNA Polymerase, HQ
T7 RNA Polymerase, HQ is a high-quality DNA-dependent RNA polymerase that exhibits high specificity for the bacteriophage T7 promoter sequence 5'-TAATACGACTCACTATA-3'. The enzyme uses double-stranded DNA containing T7 promoter sequence as a template and incorporates labeled or unlabeled nucleoside triphosphates to synthesize a single-stranded RNA transcript.
T7 RNA Polymerase, HQ is a high-quality DNA-dependent RNA polymerase that exhibits a high processivity and transcription frequency, comparable to RUO-grade T7 RNA Polymerase ver.2.0 (Cat. #2541A). However, T7 RNA Polymerase, HQ has stricter quality standards than the RUO grade (see the table below) with a high volume (1 mL/tube) for your scale-up process.
T7 RNA Polymerase, HQ exhibits high specificity for the bacteriophage T7 promoter sequence 5′-TAATACGACTCACTATA-3′. The enzyme uses double-stranded DNA containing T7 promoter sequence as a template and incorporates labeled or unlabeled nucleoside triphosphates to synthesize a single-stranded RNA transcript.
This enzyme is intended for use in the preparation of active pharmaceutical ingredients (APIs) for non-clinical trials, as well as for the process development of drug manufacturing under good manufacturing practice (GMP) regulations. Its similarity to GMP grade enzymes supports a smooth transition from RUO to GMP grade RNA manufacturing.
It is free from human- or animal-derived materials, as well as free from β-lactam compounds in the final formulation. This makes it suitable for use in applications where there is a risk of immunogenic reactions.
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This kit can be used for synthesis of RNA to be used in many applications:
- RNA for enzymatic capping
- Capped mRNA using a cap analog
- Long non-coding RNA
- Guide RNA
- siRNA precursors
- RNA standard templates for RT-qPCR
- Highly labeled RNA probes
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Product Components List to determine kit components. Certificates of Analysis and Product Components Lists are located under the Documents tab.
High Quality (HQ) products
- High quality grade and consistency
- Higher volume than RUO reagents for scale-up production
- More similar to GMP grade enzymes than RUO grade enzymes for a smooth transition to GMP grade manufacturing
- A reliable option for process development
Takara Bio's High Quality (HQ) reagents are manufactured to a higher standard than RUO grade products, without meeting all the requirements for GMP grade. The table and grade descriptions below detail the differences between these three categories of product grade.
Table 1. Comparison of Takara's RUO, HQ (high quality), and GMP grades. Quality features present in each grade are indicated by a checkmark. HQ grade has nearly as many quality features as the GMP grade and significantly more quality features than the RUO grade.
|Quality feature||Evaluation stage||RUO grade||HQ grade||GMP grade|
|Exonuclease/Endonuclease free||Final formulation|
|AOF: animal- and human-origin free||Final formulation||-|
|β-lactam free||Final formulation||-|
Table 2. Requirement compliance of Takara's RUO, HQ (high quality), and GMP grades.
|Evaluation stage||RUO grade||HQ grade||GMP grade|
|Raw materials||ISO 9001:2015||ISO 9001:2015||PIC/S GMP|
|Manufacturing process||ISO 9001:2015||ISO 9001:2015||PIC/S GMP|
RUO grade reagents are manufactured to meet the quality standards of general research reagents.
HQ grade reagents are manufactured to many of the same quality standards as GMP grade reagents, but may not be manufactured in a GMP facility. HQ grade reagents are a good choice for researchers who need high quality reagents but do not need GMP grade reagents, such as non-clinical tests or process development.
GMP grade reagents are manufactured and quality controlled in accordance with the GMP guidelines of PIC/S (Pharmaceutical Inspection Agreement and Pharmaceutical Inspection Joint Scheme). GMP grade reagents are suitable for manufacturing active pharmaceutical ingredients for clinical trials.
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