Anti-CD3 monoclonal antibody (OKT3)
The anti-CD3 monoclonal antibody (OKT3) recognizes CD3, a human glycoprotein and member of the Ig superfamily—primarily expressed on T cells, some NKT cells, and thymocytes during T-cell differentiation. Binding of an anti-CD3 monoclonal antibody to CD3 stimulates T-cell activation. Anti-CD3 antibodies (the OKT3 clone in particular) are used to expand T cells in culture for use in adoptive immunotherapy. CD8+ T-cell expansion can be further enhanced by costimulation with RetroNectin reagent.
The anti-CD3 monoclonal antibody (OKT3) recognizes CD3, a human glycoprotein and member of the Ig superfamily—primarily expressed on T cells, some NKT cells, and thymocytes during T-cell differentiation. Binding of an anti-CD3 monoclonal antibody to CD3 stimulates T-cell activation. Anti-CD3 antibodies (the OKT3 clone in particular) are used to expand T cells in culture for use in adoptive immunotherapy. CD8+ T-cell expansion can be further enhanced by costimulation with RetroNectin reagent.
This product is manufactured as a quality-assured product according to guidelines for Good Manufacturing Practice (GMP) for Investigational Products, and is, therefore, suitable for ex vivo cell culture processing and gene therapy studies.
Overview
- GMP-grade anti-CD3 mouse monoclonal antibody, free of animal-derived components
- Clinical-grade anti-CD3 mAb (GMP) can be used for ex vivo cell culture processing (investigational use only); please complete and return this form prior to purchase
- Can be used in combination with RetroNectin (GMP) to enhance T-cell (CD8+) expansion efficiency in culture
More Information
Applications
- T-cell expansion
Source
Mouse hybridoma cells
Purity
>95% by HPLC
Storage
2–8°C
Form
1.0 mg/ml sterilized solution
Product citations
Chono, H. et al. Optimization of lentiviral vector transduction into peripheral blood mononuclear cells in combination with the fibronectin fragment CH-296 stimulation. J. Biochem. 149, 285–292 (2011).
Ciceri, F. et al. Infusion of suicide-engineered donor lymphocytes after family haploidentical haemopoietic stem-cell transplantation for leukaemia (the TK007 trial): a non-randomized phase I-II study. Lancet Oncol. 10, 489–500 (2009).
Dodo, K. et al. An Efficient Large-Scale Retroviral Transduction Method Involving Preloading the Vector into a RetroNectin-Coated Bag with Low-Temperature Shaking. PLoS One 9, e86275 (2014).
Dudley, M. E. et al. Adoptive cell transfer therapy following non-myeloablative but lymphodepleting chemotherapy for the treatment of patients with refractory metastatic melanoma. J. Clin. Oncol. 23, 2346–57 (2005).
Kawamura, A. et al. Six-year Disease-free Survival of a Patient with Metastatic Eyelid Squamous Cell Carcinoma and Colon Adenocarcinoma after Repeated Postoperative Adoptive Immunotherapy. Jpn. J. Clin. Oncol. 30, 267–271 (2000).
Lamers, C. H. J., Willemsen, R. A., Luider, B. A., Debets, R. & Bolhuis, R. L. H. Protocol for gene transduction and expansion of human T lymphocytes for clinical immunogene therapy of cancer. Cancer Gene Ther. 9, 613–623 (2002).
Li, J. et al. Autologous Cytokine-induced Killer Cell Transfusion in Combination With Gemcitabine Plus Cisplatin Regimen Chemotherapy for Metastatic Nasopharyngeal Carcinoma. J. Immunother. 35, 189–195 (2012).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
T-cell expansion
RetroNectin Recombinant Human Fibronectin Fragment and RetroNectin GMP grade have widespread use as enhancers of retroviral or lentiviral gene transfer into hard-to-transduce cell types. Here, we present data on an additional application—the improved expansion of T cells from PBMCs and increased production of naïve T cells—using a combination of our products: RetroNectin reagent, GT-T551 T-cell medium, CultiLife culture bags, and anti-CD3 antibody.
T-cell expansion tech note Related productsTakara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2018 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
