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  • ‹ Back to T-cell transduction and culture
  • RetroNectin reagent
  • Anti-CD3 antibody (OKT3)
  • CultiLife culture bags
  • T-cell media
Selection guides Virus kits: product finder tool
Resources to learn about viral transduction tools Viral transduction learning center
T-cell engineering ssDNA kit used in groundbreaking CRISPR/Cas9 T-cell study!
Protocols CultiLife culture bag protocol
Home › Products › Gene function › Viral transduction › T-cell transduction and culture › Anti-CD3 antibody (OKT3)

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Anti-CD3 monoclonal antibody (OKT3)

OKT3, a monoclonal IgG2a antibody that specifically reacts with the T cell receptor-CD3 complex on the surface of circulating human T cells

The anti-CD3 monoclonal antibody (OKT3) recognizes CD3, a human glycoprotein and member of the Ig superfamily—primarily expressed on T cells, some NKT cells, and thymocytes during T-cell differentiation. Binding of an anti-CD3 monoclonal antibody to CD3 stimulates T-cell activation. Anti-CD3 antibodies (the OKT3 clone in particular) are used to expand T cells in culture for use in adoptive immunotherapy. CD8+ T-cell expansion can be further enhanced by costimulation with RetroNectin reagent.

The anti-CD3 monoclonal antibody (OKT3) recognizes CD3, a human glycoprotein and member of the Ig superfamily—primarily expressed on T cells, some NKT cells, and thymocytes during T-cell differentiation. Binding of an anti-CD3 monoclonal antibody to CD3 stimulates T-cell activation. Anti-CD3 antibodies (the OKT3 clone in particular) are used to expand T cells in culture for use in adoptive immunotherapy. CD8+ T-cell expansion can be further enhanced by costimulation with RetroNectin reagent.

This product is manufactured as a quality-assured product according to relevant guidelines for Good Manufacturing Practice (GMP), and is, therefore, suitable for ex vivo cell culture processing and gene therapy studies. 

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Cat. # Product Size Price License Quantity Details
T210 Anti-CD3 mAb GMP grade 1 mg/1 mL $1530.00

License Statement

ID Number  
M69b Before purchasing this product, you must submit a completed copy of the Anti-CD3 mAb (GMP) Order Check Sheet to us. A copy of the Anti-CD3 mAb GMP grade product License Agreement can be found by clicking here.

Anti-CD3 antibodies are commonly used in T lymphocyte proliferation protocols. Among many available clones, "OKT3" (mouse IgG2a) is the most commonly used. This monoclonal anti-CD3 antibody (clone OKT3) is manufactured as a quality-assured product, according to relevant guidelines for Good Manufacturing Practice (GMP). This product can be used for ex vivo cell culture processing in preclinical research and investigational phase studies.

Notice to purchaser

This product is to be used for research use only, including non-commercial manufacturing for clinical research. This product is not intended for humans or animals in-vivo applications. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

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Ex vivo T cell expansion from peripheral blood mononuclear cells (PBMC) was performed in the presence of anti-CD3 antibody alone or a combination of anti-CD3 and RetroNectin reagent

Ex vivo T cell expansion from peripheral blood mononuclear cells (PBMC) was performed in the presence of anti-CD3 antibody alone or a combination of anti-CD3 and RetroNectin reagent
Ex vivo T cell expansion from peripheral blood mononuclear cells (PBMC) was performed in the presence of anti-CD3 antibody alone or a combination of anti-CD3 and RetroNectin reagent. After expansion, the percentage of naïve T cells was assessed by expression CD45RA and CCR7.

RetroNectin reagent RetroNectin learning center
Technical notes T-cell expansion with RetroNectin
GT-T551 medium for T-cell culture T-cell media

Overview

  • GMP-grade anti-CD3 mouse monoclonal antibody, free of animal-derived components
  • Clinical-grade anti-CD3 mAb (GMP) can be used for ex vivo cell culture processing (investigational use only); please complete and return this form prior to purchase
  • Can be used in combination with RetroNectin (GMP) to enhance T-cell (CD8+) expansion efficiency in culture

More Information

Applications

  • T-cell expansion

Source

Mouse hybridoma cells

Purity

>95% by HPLC

Storage

2–8°C

Form

1.0 mg/ml sterilized solution

Product citations

Chono, H. et al. Optimization of lentiviral vector transduction into peripheral blood mononuclear cells in combination with the fibronectin fragment CH-296 stimulation. J. Biochem. 149, 285–292 (2011).

Ciceri, F. et al. Infusion of suicide-engineered donor lymphocytes after family haploidentical haemopoietic stem-cell transplantation for leukaemia (the TK007 trial): a non-randomized phase I-II study. Lancet Oncol. 10, 489–500 (2009).

Dodo, K. et al. An Efficient Large-Scale Retroviral Transduction Method Involving Preloading the Vector into a RetroNectin-Coated Bag with Low-Temperature Shaking. PLoS One 9, e86275 (2014).

Dudley, M. E. et al. Adoptive cell transfer therapy following non-myeloablative but lymphodepleting chemotherapy for the treatment of patients with refractory metastatic melanoma. J. Clin. Oncol. 23, 2346–57 (2005).

Kawamura, A. et al. Six-year Disease-free Survival of a Patient with Metastatic Eyelid Squamous Cell Carcinoma and Colon Adenocarcinoma after Repeated Postoperative Adoptive Immunotherapy. Jpn. J. Clin. Oncol. 30, 267–271 (2000).

Lamers, C. H. J., Willemsen, R. A., Luider, B. A., Debets, R. & Bolhuis, R. L. H. Protocol for gene transduction and expansion of human T lymphocytes for clinical immunogene therapy of cancer. Cancer Gene Ther. 9, 613–623 (2002).

Li, J. et al. Autologous Cytokine-induced Killer Cell Transfusion in Combination With Gemcitabine Plus Cisplatin Regimen Chemotherapy for Metastatic Nasopharyngeal Carcinoma. J. Immunother. 35, 189–195 (2012).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Technote describing the use of RetroNectin and CD3 antibody for

T-cell expansion

RetroNectin Recombinant Human Fibronectin Fragment and RetroNectin GMP grade have widespread use as enhancers of retroviral or lentiviral gene transfer into hard-to-transduce cell types. Here, we present data on an additional application—the improved expansion of T cells from PBMCs and increased production of naïve T cells—using a combination of our products: RetroNectin reagent, GT-T551 T-cell medium, CultiLife culture bags, and anti-CD3 antibody.

T-cell expansion tech note Related products

CultiLife culture bags protocol

Scale up transduction of hematopoietic cells with CultiLife culture bags

CultiLife culture bags provide a closed, sterile, gas-permeable system that reduces the risk of culture contamination and user exposure to cell culture materials. A 300-ml format (which can be coated with RetroNectin reagent) is available for high-efficiency viral transduction of hematopoietic cells and a 1,000-ml format can be used to expand cell cultures following transduction. View our protocol and see how to set up your cell culture in a single small bag and then expand it into multiple larger bags.

View protocol and specifications Buy now

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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