Integrase deficient lentivirus
Lentiviral vectors are widely used to efficiently transduce both dividing and quiescent cells, which makes them powerful tools for gene delivery. Most commercially available lentiviral packaging systems generate lentivirus that integrates efficiently into the target-cell genome as a provirus. However, nonintegrating lentivirus can be produced through the use of mutations in the integrase protein that minimize proviral integration.
Lentiviral vectors are widely used to efficiently transduce both dividing and quiescent cells, which makes them powerful tools for gene delivery. Most commercially available lentiviral packaging systems generate lentivirus that integrates efficiently into the target-cell genome as a provirus. However, nonintegrating lentivirus can be produced through the use of mutations in the integrase protein that minimize proviral integration. The resulting integrase deficient lentivirus (IDLV) generates circular vector episomes in transduced target cells that are gradually lost by dilution in dividing cells (transient expression), but are stable in quiescent cells. Inherently, IDLVs have a greatly reduced risk of causing insertional mutagenesis compared to integrating lentiviruses.
Lenti-X integrase deficient systems are the only fourth generation lentiviral packaging systems for IDLV generation. The system contains a combination of five plasmids mixed in optimal proportions to provide the highest possible lentiviral titers when transfected into Lenti-X 293T cells.
Lenti-X Packaging Single Shots (Integrase Deficient) provide a one-step production method to generate high-titer lentivirus. Each tube of Lenti-X Packaging Single Shots (Integrase Deficient) contains premeasured, lyophilized Xfect Transfection Reagent and an optimized formulation of Lenti-X lentiviral packaging plasmids. High-titer integrase deficient lentivirus is produced by adding the lentiviral vector of choice in sterile water to the tube, reconstituting, and then using the mixture to transfect 293T cells in a 10-cm dish.
- Produces high-titer lentivirus with a significantly reduced ability to integrate
- Transient gene expression in proliferating cells, stable expression in nondividing cells
- VSV-G pseudotype for broadest host range
- Reduced risk of insertional mutagenesis
- Use in combination with any lentiviral vector to generate nonintegrative lentivirus
- Single-shot format: prealiquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of Lenti-X lentiviral packaging plasmids that produce nonintegrating lentivirus stocks
- Published uses for IDLV include RNA interference, homologous recombination, site-specific recombination, transposition and iPS cell generation
Additional product information
Please see the product's Certificate of Analysis for information aboutstorage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Video—fourth-generation lentiviral packaging
Generate lentiviral titers that far exceed most other commercially available packaging systems—you can expect titers of 107–108 infectious units (IFU) per ml, far higher than other popular systems generate. Watch the video in the first tab to find out how our fourth-generation packaging system can generate such high titers.Learn about Lenti-X packaging Lentiviral workflow
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