Tetracycline-inducible expression: Tet-On cell lines
Tet-On cell lines are host cell lines that greatly simplify the task of setting up a tetracycline-inducible gene expression system that is activated by adding doxycycline (Dox) to the culture medium. Tet-On 3G cell lines, which are our highest-performing pre-made cell lines for this system, are located on a separate page. Tet-Off Advanced and Tet-Off inducible cell lines are also available, which are activated by removing Dox from the culture medium.
- Tet-On 3G cell lines, our highest-performing pre-made cell lines for the Tet system, are located on a separate page
- Stably expressed Tet-On transactivator protein in commonly used cell lines.
- Induce expression of your gene-of-interest by adding doxycycline (Dox) to the culture of transfected cells
- Easily create an inducible system by first placing your gene under control of a tetracycline-responsive promoter (e.g., PTRE3G), transfecting it into one of these G418-resistant cell lines, and selecting for double-stable clones.
- Inducible gene expression in mammalian cells
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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