DsRed2 fluorescent protein

Vectors for expression of the DsRed2 fluorescent protein

DsRed2 retains the benefits typical of red fluorescent proteins, such as a high signal-to-noise ratio and distinct spectral properties for use in multicolor labeling experiments. DsRed2 is a variant of Discosoma sp. red fluorescent protein (drFP583) (Matz et al. 1999) with faster maturation and lower non-specific aggregation.

DsRed2 retains the benefits typical of red fluorescent proteins, such as a high signal-to-noise ratio and distinct spectral properties for use in multicolor labeling experiments. DsRed2 is a variant of Discosoma sp. red fluorescent protein (drFP583) (Matz et al. 1999) with faster maturation and lower non-specific aggregation. The DsRed2 sequence contains a series of silent base pair changes corresponding to human codon usage preferences for high expression in mammalian cells (Haas, Park, and Seed 1996). You may also be interested in DsRed-Express2, an alternative DsRed version with mutations that enhance its solubility, reduce its green emission, and accelerate its maturation.

Overview

  • DsRed2 excitation and emission maxima are 563 and 582 nm, respectively
  • High solubility and low aggregation
  • DsRed-Express2 is an alternative DsRed version with mutations that enhance its solubility, reduce its green emission, and accelerate its maturation

More Information

Applications

  • Monitoring transfection efficiency
  • Protein localization studies
  • Multicolor experiments with AcGFP1 or other fluorescent proteins
References

Haas, J., Park, E.-C. & Seed, B. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr. Biol. 6, 315–324 (1996).

Matz, M. V et al. Fluorescent proteins from nonbioluminescent Anthozoa species. Nat. Biotechnol. 17, 969–973 (1999).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.