TransFactor kits
TransFactor kits provide a quick and sensitive ELISA-based method to detect DNA binding by a particular transcription factor, without radioactive labeling. The kits contain a 96-well plate for measuring the DNA-binding behavior of the transcription factor. Individual wells in the plate have been precoated with a consensus DNA binding sequence for the transcription factor. To perform an assay, add nuclear or cell extract from mammalian cells to the wells and incubate to allow the transcription factor to bind to its sequence. Wash away the unbound proteins, and add the primary antibody specific for the transcription factor of interest.
TransFactor kits provide a quick and sensitive ELISA-based method to detect DNA binding by a particular transcription factor, without radioactive labeling. The kits contain a 96-well plate for measuring the DNA-binding behavior of the transcription factor. Individual wells in the plate have been precoated with a consensus DNA binding sequence for the transcription factor. To perform an assay, add nuclear or cell extract from mammalian cells to the wells and incubate to allow the transcription factor to bind to its sequence. Wash away the unbound proteins, and add the primary antibody specific for the transcription factor of interest.
The TransFactor NFATc1 Kit is available in a chemiluminescent format that uses a horseradish peroxidase-conjugated secondary antibody and chemiluminescent substrate. You can immediately measure chemiluminescent intensity using any standard luminometer or CCD camera. Increasing the amount of nuclear extract containing the transcription factor added to the well will increase the signal. By adding competitor oligo, the signal is decreased, indicating the specificity of the interaction. For quantitative results, a standard curve can be generated using purified transcription factor.
Overview
- Highly sensitive detection of DNA-transcription factor interactions
- Faster and more sensitive than gel-shift assays
- Nonradioactive, chemiluminescent detection in a flexible, high-throughput format
- Compatible with nuclear, whole-cell, and cytosolic extracts
More Information
Applications
- Monitor signal transduction
- Perform multiple experiments in 3–4 hours, without the need for radioactive labeling
- Study DNA-NFATc1 transcription factor interactions
- Examine NFATc1 behavior in a cell line that has been treated with different stimuli
- Compare the relative amounts of the transcription factor NFATc1 in different cell types
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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