Methylation-dependent restriction enzyme McrBC
McrBC specifically cleaves DNA containing methyl cytosine (mC : 5-hydroxymethylcytosine, 5-methylcytosine, 4-methylcytosine) preceded by a purine nucleotide (Pu: A or G). DNA cleavage by McrBC requires at least two PumC sites within a distance of 40–2,000 bp. McrBC requires GTP for cleavage.
McrBC specifically cleaves DNA containing methyl cytosine (mC : 5-hydroxymethylcytosine, 5-methylcytosine, 4-methylcytosine) preceded by a purine nucleotide (Pu: A or G). DNA cleavage by McrBC requires at least two PumC sites within a distance of 40–2,000 bp. McrBC requires GTP for cleavage.
DNA restriction enzymes from Takara Bio are unsurpassed in quality and purity. We’ve been producing them for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests. Takara Bio DNA restriction enzymes are supplied with optimized buffers that provide maximum activity during restriction enzyme digestion. Visit our restriction enzyme resource pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.
Overview
- Source: recombinant proteins expressed in E. coli
- Concentration: 1 unit /µl
- Supplied buffer: McrBC
- Reaction temperature: 37°C
- Substrate for unit definition: pBR322
- Effect of DNA methylation: This enzyme specifically cleaves DNA containing methyl cytosine (mC : 5-hydroxymethylcytosine, 5-methylcytosine, 4-methylcytosine) preceded by a purine nucleotide (Pu: A or G). DNA cleavage by McrBC requires at least two PumC sites within a distance of 40–2,000 bp.
More Information
Applications
- DNA methylation analysis (qAMP: quantitative analysis of DNA methylation using real-time PCR)
- Enrichment of unmethylated DNA (CHARM: Comprehensive high-throughput arrays for relative methylation)
Components
- 10X McrBC Buffer 500 μl
- 0.1% BSA 1 ml
- 100 mM GTP (100×) 50 μl
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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