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Quick and easy mRNA purification: Magnosphere UltraPure mRNA Purification Kit
The Magnosphere UltraPure mRNA Purification Kit is designed for mRNA purification from total RNA derived from cultured cells or animal tissues. This mRNA isolation kit includes Magnosphere oligo-dT beads (JSR Life Sciences Corporation) that are capable of isolating intact, high-purity, polyA+ RNA with an extremely low rate of ribosomal RNA (rRNA) contamination.
The Magnosphere UltraPure mRNA Purification Kit is designed for mRNA purification from total RNA derived from cultured cells or animal tissues. This mRNA isolation kit includes Magnosphere oligo-dT beads (JSR Life Sciences Corporation) that are capable of isolating intact, high-purity, polyA+ RNA with an extremely low rate of ribosomal RNA (rRNA) contamination. The beads have a uniform particle size and super paramagnetism, allowing efficient isolation on a magnetic plate without the need for centrifugation. Compared with conventional magnetic particles, this product has lower nonspecific adsorption of impurities on the particle surface, allowing specific and efficient recovery of polyA+ RNA from even trace amounts of total RNA.
- High-quality polyA+ RNA with low rRNA contamination can be recovered with a yield of 0.5% to 2% from 5 to 50 µg of total RNA
- No phenol or other organic solvents
- Intact polyA+ RNA obtained quickly
- mRNA isolation and purification from total RNA samples
- High-throughput polyA+ RNA isolation
- Preparing polyA+ RNA with low rRNA contamination for downstream applications including high-speed sequencing and cDNA synthesis with random primers
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
The better way to isolate RNA
Critical factors when purifying RNA are the complete removal of contaminating DNA (gDNA) and the immediate prevention of degradation of RNA. The NucleoSpin RNA Plus kit introduces the NucleoSpin gDNA Removal Column, a spin column which quickly and completely removes genomic DNA contamination without the need for DNase digestion. To maintain RNA integrity, cells and tissues are first lysed by incubation in a chaotropic ion lysis buffer solution, which immediately inactivates RNases.Learn more Buy now
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