Shrimp alkaline phosphatase (SAP)
Shrimp alkaline phosphatase (SAP) nonspecifically catalyzes the dephosphorylation of 5'-termini DNA and RNA phosphomonoesters, but does not degrade diphosphate or triphosphate linkages. The enzyme acts on 5' protruding, recessive, and blunt 5'-termini. Unlike Calf intestinal alkaline phosphatase, SAP is irreversibly heat inactivated at 65°C for 15 minutes, allowing for streamlining of cloning experiments.
- Dephosphorylation of DNA 5'-termini
- Reduction of vector re-ligation and background
Olsen, R. L., Overbø, K. & Myrnes, B. Alkaline phophatase from the hepatopancreas of shrimp (Pandalus borealis): A dimeric enzyme with catalytically active subunits. Comp. Biochem. Physiol. Part B Comp. Biochem. 99, 755–761 (1991).
Additional product information
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