pRI 909 and pRI 910 binary vectors

The Ri pRI 909 and pRI 910 binary vectors are intended for Agrobacterium-mediated plant transformation and contain a region of T-DNA for Arabidopsis, tomato, tobacco, and rice transformation. The origin of the T-DNA is the vir region-lacking Rhizobium (Agrobacterium) rhizogenes Ri plasmid. pRI 909 and pRI 910 are also shuttle vectors and replicate autonomously in E. coli and Rhizobium (Agrobacterium).

In E. coli, these vectors are capable of achieving a high copy number because they contain the ColE1 replication origin. The ColE1 ori, as well as the mutant-type Ri plasmid replication of origin (Ri-ori), are each stably maintained in Rhizobium (Agrobacterium). These vectors also contain the kanamycin-resistance gene NPTIII for selection in E. coli and Rhizobium (Agrobacterium), and a mutant-type kanamycin-resistance gene NPTII for selection in plants.

The pRI 909 and pRI 910 vectors are capable of plant transformation in combination with Rhizobium (Agrobacterium) through a binary vector system. Stable plant chromosome target gene integration is possible with pRI 909 and pRI 910 because their cloning sites are located in close proximity to the T-DNA Right Border (RB) and not the NPTII plant selection marker. As a result, the target gene is not deleted.

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Cat. #	Product	Size
3261	pRI 910 DNA	10 ug	USD $455.00
pRI 909/910 DNA are binary vectors that have a region of T-DNA for plant transformation. This originated from Ri plasmid of Rhizobium (Agrobacterium tumefaciens) rhizogenes, lacking a vir region. pRI 909/910 DNA are also shuttle vectors and replicate autonomously in E. coli and Rhizobium (Agrobacterium). In E. coli, these vectors are propagated at high copy number due to the presence of ColE1 ori. pR1909/910 are maintained stably in Rhizobium (Agrobacterium) due to mutant type replication origin of Ri plasmid (Ri-ori). These vectors include NPTIII to confer Kanamycin resistance as the selection marker for E. coli and Rhizobium (Agrobacterium), a mutant type kanamycin resistant gene NPTII as the selection marker for plant, and the same multi-cloning sites as pUC type plasmids. The vectors are available for Agrobacterium-mediated binary plant transformation. Stable chromosomal integration of a target gene is possible because the cloning site is located at the position closer to Right Border (RB) of T-DNA than the selection marker (NPT II) for the plant, so the target gene is not deleted. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 3261: pRI 910 DNA
3260	pRI 909 DNA	10 ug	USD $455.00
pRI 909/910 DNA are binary vectors that have a region of T-DNA for plant transformation. This originated from Ri plasmid of Rhizobium (Agrobacterium tumefaciens) rhizogenes, lacking a vir region. pRI 909/910 DNA are also shuttle vectors and replicate autonomously in E. coli and Rhizobium (Agrobacterium). In E. coli, these vectors are propagated at high copy number due to the presence of ColE1 ori. pR1909/910 are maintained stably in Rhizobium (Agrobacterium) due to mutant type replication origin of Ri plasmid (Ri-ori). These vectors include NPTIII to confer Kanamycin resistance as the selection marker for E. coli and Rhizobium (Agrobacterium), a mutant type kanamycin resistant gene NPTII as the selection marker for plant, and the same multi-cloning sites as pUC type plasmids. The vectors are available for Agrobacterium-mediated binary plant transformation. Stable chromosomal integration of a target gene is possible because the cloning site is located at the position closer to Right Border (RB) of T-DNA than the selection marker (NPT II) for the plant, so the target gene is not deleted. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Vector map for pRI909 and pRI910 *Schematic representation of the Agrobacterium-mediated plant transformation vectors pRI909 and pRI910.* The T-DNA contains a mutant-type kanamycin resistance gene, NPTII, for selection in plants under the control of the NOS promoter and terminator with a multiple cloning site (MCS) for cloning your gene of interest. The vectors also contain a ColE1 ori, as well as the mutant-type Ri plasmid replication of origin (Ri-ori) for replication in E. coli and Agrobacterium respectively, and the kanamycin resistance gene NPTIII for bacterial selection. Back MCS sequence for pRI909 and pRI910 vectors *DNA sequence of the MCS of Agrobacterium* transformation vectors pRI909 and pRI910.** The restriction sites are shown. The sequence of the MCS and its neighboring region in pRI909 and pRI910 are orientated in the opposite directions. The M1–M4 sites show the sequences used by these M13 forward sequencing primers, while the RV site represents the M13 reverse sequencing primer location. Back 3260: pRI 909 DNA

Overview

Easy and stable plant chromosome target gene integration
Improved agrobacterium-mediated plant transformation
Applicable to various plants such as Arabidopsis, tomato, tobacco, and rice

More Information

Applications

Rhizobium (Agrobacterium)-mediated plant transformation
Arabidopsis, tomato, tobacco, rice transformation

Storage

–20°C

Properties

Preparation: column purified
Size: 9,168 bp

Form

10 mM, Tris-HCl (pH 8.0), 1 mM EDTA

Product citations

Nishiguchi, R., Takanami, M., and Oka, A. Characterization and sequence determination of the replicator region in the hairy-root-inducing plasmid pRiA 4b. Mol. Gen. Genet. 206, 1–8 (1987).

Ohba, T., Yoshioka, Y., Machida, C., and Machida, Y. DNA rearrangement associated with the integration of T-DNA in tobacco: an example for multiple duplications of DNA around the integration target. The Plant J. 7, 157–164 (1995).

Ooms, G. et al. Octopine Ti-plasmid deletion mutants of Agrobacterium tumefaciens with emphasis on the right side of the T-region Plasmid 7, 15–29 (1982).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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pRI 909 and pRI 910 binary vectors

Notice to purchaser

3261: pRI 910 DNA