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  • ‹ Back to Ligation kits
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LA PCR in vitro Cloning Kit

LA PCR in vitro Cloning Kit for long range PCR and cloning of plasmid, cDNA or genomic DNA.

The LA PCR in vitro Cloning Kit is designed specifically for long-range PCR and cloning of plasmid, cDNA, or genomic DNA regions that contain a known sequence flanking one end. This cloning kit for long fragments uses the Takara LA Taq enzyme for longer amplification and improved fidelity. Amplification is accomplished via cassette-mediated PCR whereby long DNA fragments, such as those found in genomic DNA, are used without the need for time-consuming library construction and screening. Users will need to design and prepare primers corresponding to the known region of the DNA to be amplified.

Cat. # Product Size License Quantity Details
RR015 LA PCR™ in vitro Cloning Kit 10 Rxns USD $516.00

LA PCR in vitro Cloning Kit is an improved cloning system to specifically amplify long unknown regions of cDNA and genomic DNA. Since LA PCR Technology provides high fidelity, this kit reduces the occurence of mutations during cloning. Long target DNA fragments such as genomic DNA can be easily obtained using this kit, without constructing or screening a library.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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RR015: LA PCR in vitro Cloning Kit

RR015: LA PCR in vitro Cloning Kit

More Information

Applications

  • Long-fragment cloning
  • Long-range PCR and cloning

Product citations

Deeraksa, A. et al. Characterization and spontaneous mutation of a novel gene, polE, involved in pellicle formation in Acetobacter tropicalis SKU1100. Microbiology 151, 4111–20 (2005).

Hayashi, M., Ishibashi, T., Tanaka, K. & Kasahara, M. The mouse genes encoding the third pair of beta-type proteasome subunits regulated reciprocally by IFN-gamma: structural comparison, chromosomal localization, and analysis of the promoter. J. Immunol. 159, 2760–70 (1997).

Isegawa, Y. et al. Selective amplification of cDNA sequence from total RNA by cassette-ligation mediated polymerase chain reaction (PCR): application to sequencing 6.5 kb genome segment of hantavirus strain B-1. Mol. Cell. Probes 6, 467–75 (1992).

Jansen, W. T. M. et al. Novel mobile variants of staphylococcal cassette chromosome mec in Staphylococcus aureus. Antimicrob. Agents Chemother. 50, 2072–8 (2006).

Li, H.-Y. et al. Colonization by the arbuscular mycorrhizal fungus Glomus versiforme induces a defense response against the root-knot nematode Meloidogyne incognita in the grapevine (Vitis amurensis Rupr.), which includes transcriptional activation of the class III chitinase gene VCH3. Plant Cell Physiol. 47, 154–63 (2006).

Sonke, T. et al. L-selective amidase with extremely broad substrate specificity from Ochrobactrum anthropi NCIMB 40321. Appl. Environ. Microbiol. 71, 7961–73 (2005).

Tam, W. et al. Mutational analysis of PRDM1 indicates a tumor-suppressor role in diffuse large B-cell lymphomas. Blood 107, 4090–100 (2006).       

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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  • High-throughput qPCR solutions
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  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
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