Single strand DNA Ligase
Single strand DNA Ligase is a highly efficient enzyme that catalyzes the formation of phosphodiester bonds to connect single-stranded nucleic acids. It effectively ligates single-stranded DNA (ssDNA) to ssDNA, ssDNA to single-stranded RNA (ssRNA), and ssRNA to ssRNA. Unlike conventional products, this ligase makes it easy to connect ssDNA molecules, producing linear ligation products suitable for a wide range of applications, including NGS library preparation.
Single strand DNA Ligase is a highly efficient enzyme that catalyzes the formation of phosphodiester bonds to connect single-stranded nucleic acids. It effectively ligates single-stranded DNA (ssDNA) to ssDNA, ssDNA to single-stranded RNA (ssRNA), and ssRNA to ssRNA. Unlike conventional products, this ligase makes it easy to connect ssDNA molecules, producing linear ligation products suitable for a wide range of applications, including NGS library preparation.
Single strand DNA Ligase can function in two modes: 1) an ATP-dependent reaction to join 5'-phosphorylated termini to 3'-hydroxyl termini, and 2) a faster, more efficient ATP-independent reaction with 5'-adenylated (App) adapters. This versatility makes it ideal for applications such as sample preparation for next-generation sequencing (NGS), adapter ligation, 3'-OH end labeling, and the synthesis of long or chimeric oligonucleotides.
Overview
- High ligation efficiency—achieves high-efficiency intermolecular ligation, easily connecting ssDNA molecules where conventional enzymes have low activity
- Versatile substrate compatibility—ligates various combinations of single-stranded nucleic acids, including ssDNA-ssDNA, ssRNA-ssRNA, and ssDNA-ssRNA
- Linear product creation—produces linear molecules, making it ideal for applications like adapter addition and long oligo synthesis, unlike enzymes that produce circular products
- ATP-dependent and -independent options—offers reaction flexibility by ligating 5'-phosphorylated adapters in the presence of ATP or using 5'-adenylated adapters for a faster, more efficient reaction without ATP
More Information
Applications
- NGS library preparation
- Adaptor ligation
- End labeling
- Chimeric and long oligo synthesis
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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