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  • ‹ Back to In-Fusion Cloning
  • In-Fusion Snap Assembly master mixes
  • In-Fusion Snap Assembly bundles
  • In-Fusion Snap Assembly EcoDry master mixes and bundles
  • In-Fusion Cloning for high-throughput workflows
  • In-Fusion HD Cloning Plus value bundles
  • In-Fusion HD EcoDry Cloning value bundles
  • Cloning Enhancer
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Download the In-Fusion cloning infographic Download our infographic about seamless cloning
Home › Products › Cloning › In-Fusion Cloning › In-Fusion HD Cloning Plus value bundles

Cloning

  • In-Fusion Cloning
    • In-Fusion Snap Assembly master mixes
    • In-Fusion Snap Assembly bundles
    • In-Fusion Snap Assembly EcoDry master mixes and bundles
    • In-Fusion Cloning for high-throughput workflows
    • In-Fusion HD Cloning Plus value bundles
    • In-Fusion HD EcoDry Cloning value bundles
    • Cloning Enhancer
  • Competent cells
    • Stellar chemically competent cells
    • Stellar Electrocompetent Cells
    • BMH 71-18 mutS E. coli competent cells
    • Agrobacterium electrocompetent cells
  • Ligation kits
    • DNA ligation kits, quick
    • DNA ligation kit, long fragments
    • DNA Blunting Kit
    • LA PCR in vitro Cloning Kit
    • Mighty Cloning Reagent Set (Blunt End)
    • DNA Ligation Kit, Mighty Mix
    • Cassettes and cassette primers
    • Deletion Kit for Kilo-Sequencing
  • Mutagenesis kits
    • Site-directed mutagenesis
    • Random mutagenesis kit
  • Ligation enzymes
    • E. coli DNA Ligase
    • T4 DNA Ligase
    • T4 RNA Ligase
  • Restriction enzymes
    • AatII
    • AccI
    • AccII (FnuDII)
    • AccIII (BspMII)
    • AfaI (RsaI)
    • AflII
    • AluI
    • Aor13HI (BspMII, AccIII)
    • Aor51HI (Eco47III)
    • ApaI
    • ApaLI
    • BalI
    • BamHI
    • BanII (HgiJII)
    • BciT130I (EcoRII, MvaI)
    • BcnI (CauII)
    • BglI
    • BglII
    • BlnI (AvrII)
    • BmeT110I (AvaI)
    • BmgT120I (Cfr13I, AsuI)
    • Bpu1102I (EspI)
    • Bsp1286I (SduI)
    • Bsp1407I
    • BspT104I (AsuII, NspV)
    • BspT107I (HgiCI)
    • BssHII (BsePI)
    • Bst1107I (SnaI)
    • BstPI (BstEII, EcoO65I)
    • BstXI
    • Cfr10I
    • ClaI
    • CpoI (RsrII)
    • DdeI
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    • DpnI
    • EaeI (CfrI)
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    • EcoRI
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    • KpnI
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    • MboII
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    • MspI (HpaII, HapII)
    • MunI (MfeI)
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    • PshBI (VspI)
    • Psp1406I (AclI)
    • PstI
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    • ScaI
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    • SnaBI
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    • SphI
    • Sse8387I
    • SspI
    • StuI
    • TaqI (TthHB8I)
    • Van91I (PflMI)
    • VpaK11BI (AvaII)
    • XhoI
    • XbaI
    • XspI (BfaI, MaeI)
  • Modifying enzymes
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      • DNA Polymerase I (Pol I)
      • DNA Polymerase I, Klenow Fragment
      • Poly(A) Polymerase
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      • Terminal Deoxynucleotidyl Transferase
    • Nucleases
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      • Ribonuclease H (RNase H)
      • S1 Nuclease
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      • Alkaline phosphatase (calf intestinal)
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  • Linkers, primers, and cloning vectors
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    • Cloning vectors
      • E. coli-yeast shuttle vectors
      • Chloramphenicol-resistant pUC vectors
      • Kanamycin resistant pUC vectors
      • pUC118/119 vectors
      • pUC18 and pUC19 vectors
      • DNA cloning vectors
      • T-Vectors pMD20 and pMD19
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      • M13mp18 virion DNA
      • M13mp18 RF phage vector
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      • pBR322 DNA vector
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      • Lambda DNA
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        • pRI 101 DNA vectors
        • Plant transformation vectors
        • pRI 909/910 DNA
  • Agarose gel electrophoresis
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    • DNA and RNA markers
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      • ssRNA molecular weight marker
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      • MightyPrep reagent for DNA extraction
      • DEXPAT reagents
      • Fruit-mate for RNA Purification
      • DNA extraction from yeast
      • RNA extraction from yeast
      • High-Salt Solution for Precipitation (Plant)
    • Proteinase K
    • BioMasher microhomogenizer
    • Magnosphere UltraPure mRNA
Need help?
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cloning learning center Cloning learning center
Download the In-Fusion cloning infographic Download our infographic about seamless cloning

In-Fusion HD Cloning Plus Kits for seamless DNA cloning

In-Fusion HD Cloning Plus Kits

While we continue to maintain our In-Fusion HD Cloning Plus kits, we strongly recommend that you switch to the more recently developed In-Fusion Snap Assembly master mixes. These newer versions provide the same robustness and reliability you have come to expect from In-Fusion technology, but are optimized for enhanced cloning performance.

In-Fusion HD Cloning Plus products enable directional, seamless cloning of any PCR fragment into any linearized vector in a single 15-minute reaction.

While we continue to maintain our In-Fusion HD Cloning Plus kits, we strongly recommend that you switch to the more recently developed In-Fusion Snap Assembly master mixes. These newer versions provide the same robustness and reliability you have come to expect from In-Fusion technology, but are optimized for enhanced cloning performance.

In-Fusion HD Cloning Plus products enable directional, seamless cloning of any PCR fragment—or multiple fragments—into any linearized vector in a single 15-minute reaction. No additional treatment of the PCR fragment (such as restriction digestion, ligation, phosphorylation, or blunt-end polishing) is required. The efficiency of In-Fusion HD Cloning Plus is over 95%, providing confidence in your clones and enabling you to scale up for high-throughput workflows.

The In-Fusion HD enzyme premix fuses PCR-generated sequences and linearized vectors efficiently and precisely, utilizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered by designing custom primers for amplification of the desired sequences using our primer design tool. The In-Fusion enzyme mix removes nucleotides from the 3' end of the linear DNA strands. This allows complementary base pairs between two pieces of DNA to anneal, leading to fragment joining. This method can be used to clone single or multiple fragments into a single vector without subcloning. Additional applications include mutagenesis, gene synthesis, gene design, domain swapping and domain modification.

The ability to clone directly into any destination vector at any locus (without the use of restriction enzymes) eliminates the need for any further subcloning or manipulation, simplifying the cloning workflow and further enabling a transition to high-throughput applications.

In-Fusion HD Cloning Plus system components

All In-Fusion HD Cloning products contain the In-Fusion HD Enzyme Premix and reagents for control experiments. In-Fusion HD Cloning Plus also includes:

  • In-Fusion HD Enzyme Premix

    In-Fusion HD enzyme mix fuses DNA fragments efficiently and precisely by recognizing a 15-bp overlap at their ends—without the use of ligase. Provided in a liquid format as a 5X enzyme premix in 20-μl or 100-μl aliquots, depending on kit size. Note: the EcoDry kit format includes lyophilized cloning enzyme, prealiquoted in 8-well tube strips or 96-well plates, depending on kit size.
  • CloneAmp HiFi PCR Premix

    CloneAmp HiFi PCR Premix is a convenient 2X liquid master mix that offers exceptionally accurate, efficient, and fast DNA amplification. The premix contains dNTPs and an optimized buffer, allows rapid set-up of PCR reactions, and facilitates successful cloning. The polymerase master mix is provided in 625-μl aliquots in both EcoDry and liquid kits.
  • Stellar Competent Cells

    High-efficiency competent cells are essential to the success of In-Fusion Cloning. An E. coli HST08 strain is included that provides high transformation efficiency (greater than 5 x 108 cfu/µg) and complements the efficiency of all In-Fusion HD Cloning Plus kits. Cells are provided in 100-μl aliquots in individual tubes. The 96-rxn kit includes the cells prealiquoted in 96-well plates (20 μl in each well).
  • Cloning Enhancer or NucleoSpin Gel and PCR Clean-up kit

    Cloning Enhancer (CE) is an easy-to-use enzyme premix that removes background plasmid DNA and PCR residue, eliminating the need for PCR purification prior to cloning. In-Fusion HD Cloning Plus CE kits are ideal for cloning when there is a single PCR product on the gel, and can be used as part of a streamlined workflow for high-throughput systems. Cloning Enhancer is provided in 50-μl aliquots. Alternatively, NucleoSpin Gel and PCR Clean-up is suitable for gel extraction as well as PCR purification . Provided as individual columns or 96-well plate purifications, depending on kit size.
 More  Less
Cat. # Product Size Price License Quantity Details
638920 In-Fusion® HD Cloning Plus 96 Rxns USD $1520.00

The In-Fusion HD Cloning Plus kit enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. In addition to the cloning kit, this package includes: a NucleoSpin 96 PCR Clean-Up Kit, which can be used for spin-column purification of PCR products; Stellar Competent Cells, an E. coli HST08 strain that provides high transformation efficiency paired with blue-white screening capability when used with pUC plasmid vectors; and CloneAmp HiFi PCR Premix, a convenient high-fidelity PCR 2X master mix that provides exceptionally accurate and efficient DNA amplification.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources

Back

The In-Fusion HD Cloning System has been optimized for cloning large fragments

The In-Fusion HD Cloning System has been optimized for cloning large fragments
The In-Fusion HD Cloning System has been optimized for cloning large fragments. Ten out of ten colonies contain the correct insert (100% efficiency) when cloning fragments as large as 15 kb (results confirmed by colony PCR screening).

Back

The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction

The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction
The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction. Using this system, cloning up to four 1-kb fragments simultaneously is as easy as cloning a single fragment. This saves weeks that would otherwise be spent screening clones and subcloning.

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol
The In-Fusion cloning protocol.
638911 In-Fusion® HD Cloning Plus 100 Rxns USD $1588.00

The In-Fusion HD Cloning Plus kit enables high-efficiency, high-fidelity directional cloning of one or more PCR fragments into any vector. In addition to the cloning kit, this package includes: a NucleoSpin Gel and PCR Clean-Up Kit, which can be used for spin-column purification of PCR products; Stellar Competent Cells, an E. coli HST08 strain that provides high transformation efficiency paired with blue-white screening capability when used with pUC plasmid vectors; and CloneAmp HiFi PCR Premix, a convenient high-fidelity PCR 2X master mix that provides exceptionally accurate and efficient DNA amplification.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources

Back

The In-Fusion HD Cloning System has been optimized for cloning large fragments

The In-Fusion HD Cloning System has been optimized for cloning large fragments
The In-Fusion HD Cloning System has been optimized for cloning large fragments. Ten out of ten colonies contain the correct insert (100% efficiency) when cloning fragments as large as 15 kb (results confirmed by colony PCR screening).

Back

The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction

The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction
The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction. Using this system, cloning up to four 1-kb fragments simultaneously is as easy as cloning a single fragment. This saves weeks that would otherwise be spent screening clones and subcloning.

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol
The In-Fusion cloning protocol.
638910 In-Fusion® HD Cloning Plus 50 Rxns USD $936.00

The In-Fusion HD Cloning Plus kit enables high-efficiency, high-fidelity directional cloning of one or more PCR fragments into any vector. In addition to the cloning kit, this package includes: a NucleoSpin Gel and PCR Clean-Up Kit, which can be used for spin-column purification of PCR products; Stellar Competent Cells, an E. coli HST08 strain that provides high transformation efficiency paired with blue-white screening capability when used with pUC plasmid vectors; and CloneAmp HiFi PCR Premix, a convenient high-fidelity PCR 2X master mix that provides exceptionally accurate and efficient DNA amplification.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources

Back

The In-Fusion HD Cloning System has been optimized for cloning large fragments

The In-Fusion HD Cloning System has been optimized for cloning large fragments
The In-Fusion HD Cloning System has been optimized for cloning large fragments. Ten out of ten colonies contain the correct insert (100% efficiency) when cloning fragments as large as 15 kb (results confirmed by colony PCR screening).

Back

The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction

The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction
The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction. Using this system, cloning up to four 1-kb fragments simultaneously is as easy as cloning a single fragment. This saves weeks that would otherwise be spent screening clones and subcloning.

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol
The In-Fusion cloning protocol.
638919 In-Fusion® HD Cloning Plus CE 96 Rxns USD $1520.00

The In-Fusion HD Cloning Plus CE kit enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. In addition to the cloning kit, this package includes: Cloning Enhancer, which removes background plasmid DNA and PCR residue, eliminating the need for PCR insert purification prior to cloning; Stellar Competent Cells, an E. coli HST08 strain that provides high transformation efficiency paired with blue-white screening capability when used with pUC plasmid vectors; and CloneAmp HiFi PCR Premix, a convenient high-fidelity PCR 2X master mix that provides exceptionally accurate and efficient DNA amplification.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources

Back

The In-Fusion HD Cloning System has been optimized for cloning large fragments

The In-Fusion HD Cloning System has been optimized for cloning large fragments
The In-Fusion HD Cloning System has been optimized for cloning large fragments. Ten out of ten colonies contain the correct insert (100% efficiency) when cloning fragments as large as 15 kb (results confirmed by colony PCR screening).

Back

The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction

The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction
The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction. Using this system, cloning up to four 1-kb fragments simultaneously is as easy as cloning a single fragment. This saves weeks that would otherwise be spent screening clones and subcloning.

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol
The In-Fusion cloning protocol.
638918 In-Fusion® HD Cloning Plus CE 100 Rxns USD $1588.00

The In-Fusion HD Cloning Plus CE kit enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. In addition to the In-Fusion HD Cloning Kit, this package includes: Cloning Enhancer, which removes background plasmid DNA and PCR residue, eliminating the need for PCR insert purification prior to cloning; Stellar Competent Cells, an E. coli HST08 strain that provides high transformation efficiency paired with blue-white screening capability when used with pUC plasmid vectors; and CloneAmp HiFi PCR Premix, a convenient high-fidelity PCR 2X master mix that provides exceptionally accurate and efficient DNA amplification.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources

Back

The In-Fusion HD Cloning System has been optimized for cloning large fragments

The In-Fusion HD Cloning System has been optimized for cloning large fragments
The In-Fusion HD Cloning System has been optimized for cloning large fragments. Ten out of ten colonies contain the correct insert (100% efficiency) when cloning fragments as large as 15 kb (results confirmed by colony PCR screening).

Back

The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction

The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction
The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction. Using this system, cloning up to four 1-kb fragments simultaneously is as easy as cloning a single fragment. This saves weeks that would otherwise be spent screening clones and subcloning.

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol
The In-Fusion cloning protocol.
638917 In-Fusion® HD Cloning Plus CE 50 Rxns USD $948.00

The In-Fusion HD Cloning Plus CE kit enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. In addition to the In-Fusion HD Cloning Kit, this package includes: Cloning Enhancer, which removes background plasmid DNA and PCR residue, eliminating the need for PCR insert purification prior to cloning; Stellar Competent Cells, an E. coli HST08 strain that provides high transformation efficiency paired with blue-white screening capability when used with pUC plasmid vectors; and CloneAmp HiFi PCR Premix, a convenient high-fidelity PCR 2X master mix that provides exceptionally accurate and efficient DNA amplification.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources

Back

The In-Fusion HD Cloning System has been optimized for cloning large fragments

The In-Fusion HD Cloning System has been optimized for cloning large fragments
The In-Fusion HD Cloning System has been optimized for cloning large fragments. Ten out of ten colonies contain the correct insert (100% efficiency) when cloning fragments as large as 15 kb (results confirmed by colony PCR screening).

Back

The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction

The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction
The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction. Using this system, cloning up to four 1-kb fragments simultaneously is as easy as cloning a single fragment. This saves weeks that would otherwise be spent screening clones and subcloning.

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol
The In-Fusion cloning protocol.

In-Fusion Cloning Product info: In-Fusion Snap Assembly kits
Technical notes View data: In-Fusion Snap Assembly kits
Cloning learning center Cloning learning center

Overview

In-Fusion HD Cloning Plus is a complete cloning system that has been optimized to include all the reagents you need—including the cloning enzyme mix, PCR enzyme, competent cells, and Cloning Enhancer or PCR clean-up or gel extraction kit.

  • Subcloning is unnecessary—Clone any insert, into any locus, in any vector, with just one reaction
  • Highly efficient—Over 95% efficiency demonstrated with a broad range of fragment sizes, from 0.5 kb to 15 kb
  • Seamless construction—Final constructs have no extra base pairs left over (as is often the case with restriction digest or TA cloning)
  • Flexibility to clone single or multiple fragments—Clone single or multiple DNA fragments simultaneously, in a single reaction
  • Can be used to perform site-directed mutagenesis—Click here to learn how

More Information

Applications

  • PCR cloning
  • High-throughput cloning (see full range of reagent options on our high-throughput cloning page)
  • Multiple-fragment cloning
  • Gene synthesis
  • Gene design
  • Mutagenesis
  • Domain swapping
  • Domain modification

Choose the right In-Fusion Cloning Plus system for your needs

View the selection guide.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Screening for novel coronavirus with one-step RT-qPCR: getting ahead of the outbreak

Learn how researchers identified a novel, highly contagious coronavirus and developed a one-step RT-qPCR screening protocol.

Read the blog article


Selection guides Selection guide
Primer Design Tool Primer design tool
Technical notes
In-Fusion Cloning tech notes

Field tester tech notes

A successful alternative to traditional ligation cloning

Learn how In-Fusion Cloning was able to provide successful results with difficult inserts, where ligation had previously failed.

Solve a synthesis challenge with easy multiple-insert cloning

Learn more about how In-Fusion technology was able to seamlessly clone two overlapping inserts to generate an activation domain.

Outperform the TOPO cloning workflow in both speed and accuracy

Learn how In-Fusion Cloning provided better, faster results than TOPO methods, including a cloning accuracy of 100%.

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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2021 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Capturem Trypsin for a rapid, efficient mass spectometry workflow at room temperature.

Speed up your mass spec workflow

Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion.

Capturem trypsin technology

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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