- In-Fusion Cloning
- Competent cells
- Ligation kits
- Mutagenesis kits
- Ligation enzymes
- Restriction enzymes
- Modifying enzymes
- X-Gal and IPTG
- Linkers, primers, and cloning vectors
- Agarose gel electrophoresis
- Nucleic acid extraction
BMH 71-18 mutS E. coli chemically competent cells
E. coli BMH 71-18 mutS, which is mismatch-repair deficient, is used to propagate mutated plasmids. Two rounds of DNA digestion and transformation ensure that a very high frequency of transformants carry the mutated plasmid, which nearly always contains both mutations—the desired mutation and the selection mutation (Deng and Nickoloff 1992; Zhu 1996). Not available in all regions.
Deng, W. P. & Nickoloff, J. A. Site-directed mutagenesis of virtually any plasmid by eliminating a unique site. Anal. Biochem. 200, 81–8 (1992).
Haught, C., Wilkinson, D.L., Zgafas, K., and Harrison, R. G. A Method to Insert a DNA Fragment into a Double-Stranded Plasmid. Biotechniques 16, 47–8 (1994).
Zhu, L. In vitro site-directed mutagenesis using the unique restriction site elimination (USE) method. Methods Mol. Biol. 57, 13–29 (1996).
Zhu, L. & Holtz, A. E. A universal nested deletion method using an arbitrary primer and elimination of a unique restriction site. Methods Mol. Biol. 57, 119–37 (1996).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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