ApoPrimer Set (Bcl-2 family) for RT-PCR
The Bcl-2 gene family encodes a set of proteins involved in apoptotic inhibition, which are commonly analyzed during the performance of a Bcl-2 apoptosis assay. The ApoPrimer Set (Bcl-2 family) for RT-PCR is composed of a set of primers used to amplify cDNA derived from the mRNA of seven Bcl-2 family members (mcl-1, bfl-1, bax-alpha, bcl-2, bak, bik, bcl-x), as well as a beta-actin control. A positive control RNA is also included. The beta-actin primer is a useful internal control for sample quantification and for comparison between samples. PCR products generated from the positive control RNA can be distinguished from target mRNA due to size differences of the amplified products.
- Detection of human Bcl-2 gene family expression by RT-PCR
- Bcl-2 apoptosis assay
- Human mcl-1 Primer Mix (40 µl)
- Human bfl-1 Primer Mix (40 µl)
- Human bax-alpha Primer Mix (40 µl)
- Human bcl-2 Primer Mix (40 µl)
- Human bak Primer Mix (40 µl)
- Human bik Primer Mix (40 µl)
- Human bcl-x Primer Mix (40 µl)*
- Human beta-actin Primer Mix (40 μl)
- APO Positive Control RNA1 (106 copies/μl; 24 μl)
* Note: Human bcl-x Primer Mix amplifies the cDNA from both bcl-xL and bcl-xS.
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Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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